Conclusions

A new form of bovine rhodopsin crystals with p22₁2₁ symmetry was obtained and for the first time not only images but also electron diffraction pattern could be obtained.

Out of a large number of images 20 crystalline areas were chosen, amplitudes and phases were extracted and a projection map to 5 Å was calculated. The most important conclusions are:

The molecules in this crystal form seem to be more densely packed leading to higher order of these crystals.
The quality of the extracted amplitudes and phases depends on careful boxing and unbending of the crystals.
The projection amplitudes were found to be reliable to 5 Å.
The phase error of unique Fourier components and the phase difference between two halves of data showed that phases were reliable to 5 Å.
In the new projection map helix 5 seems to be more highly tilted and between helices 3 and 5 a clear peak is present suggesting that helix 3 is prolonging the arc shaped feature towards helix 5.
Additional density is detected in the region of helix 6.