Figures 2 and 3

Figure 2. Electron microscope images of negatively stained CNG channels. (A) CNG channels imaged with the normal of the electron microscope grid parallel to the electron beam. (B) Class-averages generated by classification and averaging of the particles as in (A). (C) CNG channels imaged with the normal of the electron microscope grid at 45° to the beam direction. (D) Particles imaged with the normal of the grid at 0°, 15°, 30° and 45° to the beam direction were combined and classified and averaged to generate these class-averages. The scale bars are 25nm in (A) and (C) and 10nm in (B) and (D).

Figure 3. Single particle averaging of the CNG channel. (A) raw images of the channel with different euler angles (row 1) are compared with the corresponding class-averages (row 2), the projections of the three-dimensional reconstruction (row 3) and the surface representations of the reconstruction (row 4). All of the images in one column share the same euler angles. (B) A comparison of two half data sets by Fourier Shell Colleration (FSC) suggests a resolution of 35Å, as measured by the resolution at which the FSC falls below 0.5.

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Gebhard Schertler, gfx@mrc-lmb.cam.ac.uk