EpsinR: an AP1/clathrin interacting protein

involved in vesicle trafficking


Myc-tagged and endogenous epsinR versus epsinR-GFP


endogenous EpsinR - green (Ra43)
AP1 - red
large image (51 kb)

endogenous EpsinR - blue (Ra43)
EpsinR-GFP - green,
AP1 - red
large image (33 kb)

EpsinR ENTH-GFP co-localises with AP1 at a TGN-like compartment. The N-terminally Myc-tagged protein is cytoplasmic. We do not know the reason for this discrepancy. but we see the same distribution for full-length epsinR whether it has an N-terminal Myc tag or a C-terminal GFP tag.

EpsinR ENTH-GFP - green
AP1 - red
large image (37 kb)

myc-EpsinR ENTH - green
AP1 - red
large image (94 kb)

EpsinR-GFP versus AP1 and clathrin
There is good overlap between the perinuclear epsinR-GFP and endogenous AP1 and clathrin

EpsinR GFP - green
large image (36 kb)
AP1 - red
large image (76 kb)
merge
large image (84 kb)
EpsinR GFP - green
large image (40 kb)
AP1 - red
large image (36 kb)
merge
large image (56 kb)
EpsinR GFP - green
large image (16 kb)
Clathrin - red
large image (44 kb)
merge
large image (40 kb)
EpsinR GFP - green
large image (24 kb)
Clathrin - red
large image (76 kb)
merge
large image (44 kb)

EpsinR cover page

Endogenous epsinR expression

Comparison of epsin1 and epsinR

Myc-epsinR (WT) overexpression and colocalisation with markers

Myc-epsinR D34G+R67L (lipid binding mutant)

Myc-epsinR L10E (proposed lipid conformation mutant)

Myc-epsinR D342R+D422R (clathrin binding mutant)

Myc-epsinR D34G+R67L + D342R+D422R (lipid and clathrin binding mutant)

EpsinR-GFP and ENTH domain overexpression

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