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Oligonucleotide Synthesis Service
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Selected Recent Papers from the Gait group
2011 and in press
2010
2009
2008
2007
2006
2005
2011 and in press
Overview of alternative oligonucleotide chemistries for exon skipping.
A.F. Saleh, A.A. Arzumanov and M.J. Gait,
Methods in Molecular Biology: Exon Skipping, in press.
Enhancement of exon skipping and dystrophin production by 3’-peptide conjugates of morpholino (PMO) oligonucleotides in a mdx mouse model of Duchenne muscular dystrophy.
A.F. Saleh, A.A. Arzumanov, H. Yin, C. Betts, S. Hammond, M.J.A. Wood and M.J. Gait,
Collection Symposium Series (2011) Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, in press.
Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic correction in mdx mice.
H. Yin, A, F. Saleh, C. Betts, P. Camiletti, Y. Seow, S. Ashraf, A. Arzumanov, M. J. Gait and M.J.A. Wood,
Molecular Therapy, doi:10.1038/mt.2011.79.
PepFect 14, a novel cell-penetrating peptide for oligonucleotide delivery in solution and as solid formulation.
K. Ezzat, S. El Andaloussi, E. M. Zaghloul, T. Lehto, S. Lindberg, P. M.D. Moreno, J. R. Viola, T. Magdy, R.Abdo, P. Guterstam, R. Sillard, S., M. Hammond, M. J. A. Wood, A. A. Arzumanov, M. J. Gait, C. I. E. Smith, Ü. Langel and M. Hällbrink.
Nucleic Acids Research (2011) doi: 10.1093/nar/gkr072.
MicroRNA fate upon targeting with anti-miRNA oligonucleotides as revealed by an improved Northern-blot based method for miRNA detection.
A.G. Torres, M.M. Fabani, E. Vigorito and M.J. Gait,
RNA (2011) 17, 933-943.
Diaphragm rescue alone prevents heart dysfunction in dystrophic mice.
A. Crisp, H. Yin, A. Goyenvalle, C. Betts, H.M. Moulton, Y. Seow, A. Babbs, T. Merritt, A.F. Saleh, M.J. Gait, D.J. Stuckey, K. Clarke, K.A. Davies and M.J.A. Wood,
Human Molecular Genetics (2011) 20, 413-421.
2010
Synthesis and splice-redirecting activity of branched. Arginine-rich peptide dendrimer conjugates of Peptide Nucleic Acid Oligonucleotide.
A.F. Saleh, A. Arzumanov, R. Abes, D. Owen, B. Lebleu, B. and M. J. Gait,
Bioconjugate Chem. (2010) 21, 1902-1911.
Efficient inhibition of miR-155 with peptide nucleic acids in primary B-cells and in vivo.
M.M. Fabani, C. Abreu-Goodger, D. Williams, P.A. Lyons, A. Torres, K.G.C. Smith, A. Enright, M.J. Gait and E.Vigorito,
Nucleic Acids Res. (2010) 38, 4466-4475.
RNA-targeted splice-correction for neuromuscular disease.
M.J.A Wood, M.J. Gait and H. Yin,
Brain (2010) 133, 957-972.
In vitro evaluation of novel antisense oligonucleotides is predictive of in vivo exon skipping activity for DMD.
Q. Wang, H. Yin, P. Cameletti, C. Betts, P. Kohl, H. Moulton, H. Lee, A.F. Saleh, M.J. Gait and M.J.A. Wood.
J. Gene Med. (2010) 12, 354-364.
Optimization of peptide nucleic acid antisense oligonucleotides for local and systemic dystrophin splice correction in the mdx mouse.
H. Yin, C. Betts, A. F Saleh, G. D. Ivanova, H. Lee, Y. Seow, D. Kim, M. J Gait and M J A Wood
Molecular Therapy (2010) 18, 819-827.
Cell penetrating peptides and their applications: an overview.
F.S. Hassane, A.F. Saleh, R. Abes, M.J. Gait and B. Lebleu,
Cell. Mol. Life Sci. (2010) 67, 715-726.
Splice redirection as a convenient assay to monitor CPP-ON efficiency and mechanism.
R. Abes, A.A. Arzumanov, A.F. Saleh, F.S. Hassane, M.J. Gait and B. Lebleu.
Cell-penetrating peptides. Methods and Protocols. Methods in Molecular Biology, (2010) (U. Langel, Ed) pp 307-320.
2009
A peptide-based dendrimer that enhances the splice-redirecting activity of PNA conjugates in cells.
F.S. Hassane, G.D. Ivanova, E. Boleweska-Pedyczak, R. Abes, A.A. Arzumanov, M.J. Gait, B. Lebleu, J. Gariépy,
Bioconj Chem. (2009) 20, 1523-1530.
Increased RNAi is related to intracellular release of siRNA via a covalently attached signal peptide.
M. Overhoff, A. Detzer, W. Wünsche, M. Rompf, J.J. Turner, G.D Ivanova, M.J. Gait and G. Sczakiel,
RNA (2009) 15, 627-636.
2008
Improved cell-penetrating peptide-PNA conjugates for splicing redirection in HeLa cells and exon skipping in mdx mouse muscle.
G.D. Ivanova, A.Arzumanov, R. Abes, H. Yin, M.J.A. Wood, B. Lebleu and M.J.Gait,
Nucleic Acids Res. (2008), 36, 6418-6428.
PNA-peptide conjugates as intracellular gene control agents.
G.D. Ivanova, M.M. Fabani, A.A. Arzumanov, R. Abes, H. Yin, B. Lebleu, M. Wood and M.J. Gait,
Nucleic Acids Symposium Series No 52 (2008) 31-32.
RNA targeting in cells by peptide conjugates of peptide nucleic acids (PNA).
G.D. Ivanova, A.A. Arzumanov, J.J. Turner, M.M. Fabani, R. Abes, B. Lebleu and M.J. Gait,
Collection Symposium Series (2008) Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, pp 103-111.
Arginine-rich cell penetrating peptides: design, structure-activity and applications to splicing correction by steric block oligonucleotides.
R. Abes, A. Arzumanov, H. Moulton, S. Abes, G. Ivanova, M.J. Gait, P. Iversen and B. Lebleu,
J. Peptide Sci. (2008) 14, 455-460.
Steric block high affinity oligonucleotide analogues: a new tool for mapping RNA-protein binding sites.
D. Brown, E. Joy, J. Greatorex, M. J. Gait and A.M.L. Lever
Nucleosides, Nucleotides and Nucl. Acids (2008) 27, 196-212.
miR-122 targeting with LNA/2’-O-methyl oligonucleotide mixmers, PNA and PNA-peptide conjugates,
M. Fabani and M.J. Gait
RNA, (2008) 14, 336-346.
Download Supplementary Figures here
Peptide-based delivery of steric-block PNA oligonucleotides.
S. Abes, G.D. Ivanova, R. Abes, A.A. Arzumanov, D. Williams, B. Lebleu and M. J. Gait
In Methods in Molecular Biology, vol 480 Macromolecular Drug Delivery (M.Belting,ed.) (2008) Humana Press, New York, pp. 85-99.
Cell penetrating peptide conjugates of steric block oligonucleotides.
B. Lebleu, H. Moulton, R. Abes, G. D. Ivanova, S. Abes, D.A. Stein, P.L Iversen, A.A. Arzumanov and M.J. Gait
Adv. Drug Delivery Rev. (2008) 60, 517-529.
Peptide-PNA conjugates for modulation of gene expression.
M.M. Fabani, G.D. Ivanova and M.J. Gait
in Therapeutic Oligonucleotides (2008) (J. Kurreck, ed.), Royal Society of Chemistry Cambridge pp 80-102.
2007
Comparative studies of tricyclo-DNA and LNA-containing oligonucleotides as inhibitors of HIV-1 gene expression.
G. Ivanova, A. Arzumanov, M. J. Gait, S. Reigadas, M.-L. Andreola, J.-J. Toulmé, D. Ittig and C. Leumann
Nucleosides, Nucleotides and Nucleic Acids (2007) 26, in press.
Preparation of 2’-hydrazide oligonucleotides and their reaction with aldehydes and 1,3-diketones.
T.S. Zatsepin, T.S. Oretskaya, M.J. Gait and D.A. Stetsenko
Nucleosides, Nucleotides and Nucleic Acids (2007) 26, in press
Lung delivery studies using siRNA conjugated to Tat (48-60) and Penetratin reveal peptide induced reduction in gene expression and induction of innate immunity.
S. Moschos, S.W. Jones, M. M. Perry, A.E. Williams, J.S. Erjefält, J.J. Turner, P.J. Barnes, B. S. Sproat, M.J. Gait and M.A. Lindsay
Bioconjugate Chemistry (2007) 18, 1450-1459.
MALDI-TOF mass spectral analysis of siRNA degradation in serum confirms an RNAse A-like activity.
J.J. Turner, S.W Jones, S. Moschos, M.A. Lindsay and M.J. Gait
Mol. Biosystems (2007) 3, 43 - 50.
Tricyclo-DNA containing oligonucleotides as steric block inhibitors of HIV-1 Tat-dependent trans-activation and HIV-1 infectivity.
G. Ivanova, S. Reigadas, D. Ittig, A. Arzumanov, M.-L. Andreola, C. Leumann, J.-J. Toulmé and M. J. Gait
Oligonucleotides. (2007) 17, 54-65.
Chemical modifications to improve the cellular uptake of oligonucleotides.
F. Debart, S. Abes, G. Deglane, H. M. Moulton, P. Clair, M. J. Gait, J.-J. Vasseur and B. Lebleu
Current Topics in Medicinal Chemistry (2007) 7, 727-737.
Peptide-based delivery of nucleic acids: design, mechanism of uptake and applications to splice-correcting oligonucleotides.
S. Abes, H. Moulton, J. Turner, P. Clair, J. P. Richard, P. Iversen, M. J. Gait and B. Lebleu
Biochem. Soc. Trans. (2007) 35, 53-55.
RNA targeting with peptide conjugates of oligonucleotides, siRNA and PNA,
J.J. Turner, S. Jones, M.M. Fabani, G. Ivanova, A.A. Arzumanov and M.J. Gait
Blood, Cells, Molecules and Disease (2007) 38, 1-7.
Systematic screening of LNA/2’-O-methyl chimeric derivatives of a TAR RNA aptamer.
C. Di Primo, I. Rudloff, S. Reigadas, A.A. Arzumanov, M. J. Gait and J.-J. Toulmé
FEBS Letters (2007) 581, 771-774.
Efficient splicing correction by PNA conjugation to an R6-Penetratin delivery peptide.
S. Abes, J. J. Turner, G. D. Ivanova, D. Owen, D. Williams, P. Clair, M. J. Gait and B. Lebleu
Nucleic Acids Res. (2007) 35, 4495-4502.
Lipoplex and peptide-based strategies for the delivery of steric-block oligonucleotides.S. Resina, S. Abes, J.J. Turner, P. Prevot, A. Travo P. Clair, M.J. Gait, A.R. Thierry and B. Lebleu
Int. J. Pharm. (2007) 344, 96-102.
Cell penetrating peptide-based delivery of oligonucleotides: an overview.
R. Abes, A.A. Arzumanov, H. Moulton, S. Abes, G.D. Ivanova, P.L. Iversen, M.J. Gait and B. Lebleu
Biochem. Soc. Trans. (2007) 35, 775-779.
Synthesis of oligonucleotides containing amino acid residues in the 2’-position of ribose moiety.
E.V. Kazanova, E.M. Zubin, D. Stetsenko, V. Kachalova, S.C. Holmes, M.J. Gait and T.S. Oretskaya
Russ. Chem. Bull. (2007) 56, 806-814.
2006
Disulfide conjugation of peptides to oligonucleotides and their analogues.
J.J. Turner, D. Williams, D. Owen and M.J. Gait
Curr. Protocols Nucl. Acids Chem. (2006) 4.28, 1-21.
Targeting the HIV-1 RNA leader sequence with synthetic oligonucleotides and siRNA: chemistry and cell delivery.
J.J. Turner, M. Fabani, A.A. Arzumanov, G. Ivanova and M.J. Gait
BBA Biomembranes, (2006) 1758, 290-300.
Endosome trapping limits the efficiency of splicing correction by PNA-oligolysine conjugates.
S. Abes, D. Williams, P. Prevot, A. Thierry, M. J. Gait, and B. Lebleu,
J. Controlled Release (2006) 110, 595-604.
Anti-HIV activity of steric block oligonucleotides.
G. Ivanova, A.A. Arzumanov, J.J. Turner, S. Reigadas, J.-J. Toulmé, D.E. Brown, A.M.L. Lever and M.J. Gait.
Ann. N.Y. Acad. Sci., (2006) in press.
Inhibition of HIV-1 replication by oligonucleotide analogues directed to the packaging signal and TAR.
D.E. Brown, A.Arzumanov, S. Syed, M.J. Gait and A.M.L. Lever.
Antiviral Chem. & Chemotherapy (2006) 17, 1-9.
Peptide conjugates of oligonucleotide analogues and siRNA for gene expression modulation.
J.J. Turner, A.A. Arzumanov, G. Ivanova, M. Fabani and M.J. Gait
In Handbook of Cell Penetrating Peptides, 2nd Edition (2006) CRC Press, Boca Raton, USA, pp 313-328.
Oligonucleotide analogues as antiviral agents.
M.M. Fabani, J.J. Turner and M.J. Gait
Curr. Opinion Mol. Therapeutics (2006) 8, 108-114.
Synthesis of 2’-hydrazine oligonucleotides and their efficient conjugation with aldehydes and 1,3-diketones.
T.S. Zatsepin, M.J. Gait, T.S. Oretskaya and D.A. Stetsenko
Tetrahedron Letters, (2006) 47, 5515-5518.
2005
Synthesis, cellular uptake and HIV-1 Tat-dependent trans-activation inhibition activity of oligonucleotide analogues disulphide-conjugated to cell penetrating peptides.
J.J. Turner, A. A. Arzumanov and M.J. Gait, Nucl. Acids Res. (2005) 33, 27-42.
Antiviral activity of steric-block oligonucleotides targeting the HIV-1 trans-activation response and packaging signal stem-loop RNAs.
D. Brown, A. A. Arzumanov, J.J. Turner, D.A. Stetsenko, A.M.L. Lever and M.J. Gait
Nucleosides, Nucleotides and Nucleic Acids (2005) 5-7, 393-396.
Conjugates of oligonucleotides and analogues with cell penetrating peptides as gene silencing agents.
T.S. Zatsepin, J.J. Turner, T.S. Oretskaya, M.J. Gait
Current Pharmaceutical Design (2005) 11, 3639-3654.
Use of carbonyl group addition-elimination reactions for synthesis of nucleic acids conjugates.
T.S. Zatsepin, D.A. Stetsenko, M.J. Gait and T.S. Oretskaya
Bioconjugate Chem, Bioconjugate Chem, (2005) 16, 471-489.
Synthesis of DNA conjugates by solid-phase fragment condensation via aldehyde-nucleophile coupling.
T.S. Zatsepin, D.A. Stetsenko,. M.J. Gait and T.S. Oretskaya.
Tetrahedron Lett. (2005), 46, 3191-3195.
Synthesis and properties of oligodeoxyribonucleotides containing 2´-O-(2,3-dihydroxypropyl)- and 2´-O-(2-oxoethyl)arabinouridine residues.
T. S. Zatsepin, Yu. M. Ivanova, D. A. Stetsenko, M. J. Gait, and T. S. Oretskaya.
Russ. Chem. Bull. Int. Ed. (2005) 54, 238-246.
Oligonucleotides Containing 2’-O-[2-(2,3-Dihydroxypropyl)amino-2-oxoethyl]uridine as Suitable Precursors of 2’-Aldehydo Oligonucleotides for Chemoselective Ligation.
E. M. Zubin, D.A. Stetsenko, T. S. Zatsepin, M. J. Gait
and T.S. Oretskaya.
Bioorg. Med Chem. Lett. (2005) 13, 4912-4920.
2’-Hydrazine oligonucleotides: synthesis and efficient conjugation with aldehydes.
T.S. Zatsepin, D.A. Stetsenko, M. J. Gait and T. S. Oretskaya.
Nucl. Acids Res. Symposium Series No 49 (2005) 133-134.
Incorporation of side-chain groups of basic amino acids into oligonucleotides via the 2’-position of uridine.
E.M. Zubin, D.A. Stetsenko, A.V. Kachalova, M.J. Gait and T.S. Oretskaya.
Nucleic Acids Res. Symposium Series No 49 (2005) 347-348.
Inhibition of HIV-1 Tat-dependent trans-activation by steric block oligonucleotide analogues containing amino modifications.
G. Ivanova, A.A. Arzumanov, B.R. Babu, N. Karla, D. Sweedler, L. Beigelman, J. Wengel and M.J. Gait
in Collection Symposium Series, Vol. 7 (2005), Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, 367-371.
A bifunctional siRNA construct induces RNA interference and also primes PCR amplification for its own quantification.
M. Jiang, A.A. Arzumanov. M.J. Gait and J. Milner
Nucl. Acids Res. (2005) e151.
Synthesis and oligonucleotide incorporation of nucleoside analogues containing pendant imidazolyl or amino functions. The search for sequence-specific artificial ribonucleases.
S.C. Holmes and M.J. Gait
Eur J. Org Chem, (2005) 5171-5183.
Cell-penetrating peptide conjugates of peptide nucleic acids (PNA) as inhibitors of HIV-1 Tat-dependent trans-activation in cells
J.J. Turner, G. Ivanova, B. Verbeure, D. Williams, A.A. Arzumanov, S. Abes, B. Lebleu and M. J. Gait.
Nucl. Acids Res. (2005) 33, 6837-6849. |
