RNA-protein Complex Structures
The first RNA-protein complex to be crystallised was a complex of E. coli glutaminyl-tRNA synthetase with its cognate tRNA (Rould et al.1989). Perone et al. (1988) overproduced tRNA(Gln) in E. coli, purified it to homogeneity and crystallised it with glutaminyl-tRNA synthetase. Around the same time the Strasbourg group determined the structure of the complex between yeast aspartyl-tRNA sysnthetase and its cognate tRNA (Ruff et al., 1991). The deterimation of these two structures was a major breakthrough in the field. Both structures show how the tRNA synthetase directly recognise the anticodon nucleotide. Subsequently in 1994 the structure of seryl-tRNA synthetase was determined in complex with its cognate tRNA (Bior et al., 1994). This enzyme recognises the congnate tRNA in a unique way. The extra loop of seryl-tRNA but not the anticodon loop is recognised by the enzyme.

In 1994 two more RNA-protein complex structures were reported. Valegaard et al. (1994) soaked an RNA hairpin representing the initiation site of assembly into a crystal of the MS2 bacteriophage capsid. The structure shows how the RNA hairpin binds to capsid protein around a dyad axis. We also reported the crystal structure of the spliceosome U1A protein in complex with a fragment of U1 snRNA (Oubridge et al., 1994). U1A contains an RNP domain or RRM (RNA recognition motif) which is one of the most commonly found RNA binding motif. Chemically synthesized RNA was used for both crytals. Crystal structures of more than twenty RNA-protein complexes have been reported to date. The atomic resolution structures of the 50S and 30S ribosomal subunits reprent a big step forward in our understanding of RNA-protein recognition and the archtecture of large RNA-protein assemblies
Crystallisation conditions for RNA-protein complexes
PDB complex crystallisation conditions Color Color method Å phasing references
1GTS E. coli GlnRS-tRNA complex 44-64% Na-Citrate, 20mM MgCl2, 80mM Pipes pH7.0 hanging drop at 2.8Å K2AuCl4, DiHgAc, EtHgPO4, PCMB Rould et al. 1989
1ASY

1ASZ

 yeast AspRS-tRNA complex 40mM Tris-Maleate/NaOH pH7.5, 5mM MgCl2, 25% AS equilibrated with 60% AS hanging drop at 4°C 3.0Å Hg, Au, Sm Ruff et al., 1991
1SER SerRS-tRNA complex 2.9Å Biou et al., 1994
1BBV MS2 phage-RNA complex small RNA hairpin was soaked into MS2 capsid crystal Vålegard et al., 1994
1URN spliceosomal U1A-hairpin RNA complex 1.8M AS, 50mM Tris-Cl pH 7.0, 2mM spermine hanging drop vapour diffusion 1.9Å Methylmercury derivative of a cysteine mutant Oubridge et al., 1994;1995
1TTT

1B23

 elongation factor Tu-tRNA(Phe)-GDPNP complex The ternary complex in 20mM Tris-HCl pH7.6, 3mM MES pH2.7, 35% AS, 7mM MgCl2, 0.5mM NaN3, 0.5mM DTT and 0.4mM GDPNP equilibrated with a resovoir of 47-49% AS pH 6.7-7.0 hanging drop vapour diffusion at 4°C 2.7Å molecular replacement Nissen et al., 1995; Nissen et al., 1994; Nissen et al., 1999
T thermophilus LysRS-tRNA(Lys) complex 24-26% AS, 50mM Tris-malate pH 7.6, 10mM MgCl2, amM NaN3, ? 2.9Å molecular replacement Cusack et al., 1996; Yaremchuk et al., 1995
1AV6 Vaccinia cap modifying enzyme with M7Gpppgaaaaa 15-20% PEG8000, 0.1M Cac pH7.0, 0.125M NH4SO4 hanging drop 2.7Å molecular replacement Hodel et al.
1A9N Spliceosomal U2B"-U2A'-hairpin RNA complex 50mM NaCl, 9mM MgCl2, 0.25mM spermine, 0.25% n-octylglucopyranoside, Tris-Cl pH 7.3, 1% PEG600, sitting drop vapour diffusion 2.4Å Methylmercury derivative of a cysteine mutant Price et al., 1998
1MMS L11-RNA complex 15% PEG4000, 0.2M KCl, 50 mM MgCl2, 20mM CdCl2, 1mM DTT, 50mM Tris-Cl pH 7.5 hanging drop vapour diffusion at 22°C 2.6Å MeHg MAD Wimberly et al., 1999
1QA6

1HC8

 L11(fragment)-RNA complex 15% PEG 600, 80mM magnisium acetate, 0.2mM cobalt hexamine chloride, 50mM sodium cacodylate pH6.5, 100mM KCl sitting drop at 37°C 2.8Å Se-Met Conn et al, 1999
1DI2 dsRBD-RNA complex 225mM KCl, 50 mM NaCl, 1% PEG4000, 12% ethyleneglycol, 100mM MES pH5.4, 10 mM DTT, 10mM mercaptoethanol hanging drop vapour diffusion 1.9Å 5-Iodedeoxycytidine Ryter and Schelts, 1998
1CX0 hepatitis delta virus ribozyme-U1A complex 12.5-14% PEG-MME2000, 100mM Tris-HCl pH 7.0, 200-250mM Li2SO4, 4mM spermine-HCl with 0.2mM Co(III) hexamine, 0.4mM Os(III) hexammine chloride triflate or 5mM MgCl2 sitting drop vapour diffusion 2.3Å MAD with sellenomethionine labelled U1A protein Ferre-A'Amare et al. 1998
1HVU HIV-1 reverse transcriptase-RNA pseudoknot complex Complex in 50mM bis-Trispropane pH 7.0, 100mM AS, 0.02% hexylglucopyranoside, 10% glycerol was mixed with equal volumer of well solution (1.0-1.1M Na citrate pH 6.9, 100mM Mg acetate, 3% dioxane hanging drop vapour diffusion at 4°C 4.8Å Molecular replacement Jaeger et al., 1998
2FMT methionyl-tRNA(Met) transformylase complex 23% PEG-MME5000, 0.2M AS, 8% ethyleneglycol, 10mM MgCl2, 50mM KCl, 50mM MOPS buffer pH6.6 macro-seeding 2.8Å SIR with PCMBS Schmitt et al., 1998
1A8V Rho Transcription Termination Factor/RNA (CCCCC) Complex 10-20% MPD, 50-100mM NH4 acetate, 0.1M Tris pH8.5, 4mM Cupric chloride, 2mM erbium acetate hanging drop at 30°C 2.4Å MR using unliganded Rho 13N Bogden et al., 1999
1C9S

1GTF

1GTN

 TRAP-RNA complex 9-12% PEG-MME2000, 0.2M K-glutamate, 50mM triethanolamine pH 8.0, 10mM MgCl2, hanging drop vapour diffution 1.9Å Molecular replacement using AMoRe Antson et al. 1999
1B7F Sxl-traRNA complex 10% PEG6000, 50mM NaMOPS pH7.0, 100mM LiSO4, 3mM spermine, 1-3% dioxane, 2mM Zn acetate, 2mM MgCl2 hanging drop vapour diffusion 2.6Å SeMet, K2PtCl4,K3UO2F5 Handa et al. 1999
1CVJ PABP-polyA complex 2.1M AS, 100mM Tris-Cl pH8.0 hanging drop vapour diffusion at 24°C 2.6Å SmAc3, K2IrCl6 Deo et al.,1999
1DRZ P4-P6 domain 25% MPD, 100mM K cacodylate pH 6.0, 50 mM MgCl2, 0.5 mM spermine, 10% isopropanol, 0.05-0.1 mM Co hexamine chloride, sitting drop micro-seeding 2.8Å Osmium hexammine triflate Cate et al., 1996; Doudna et al., 1993
1QU2

1QU3

1FFY

 IleRS-tRNA(Ile)-mupirocin complex Complex in 10mM bicine pH8.0, 25mM KCl, 12.5mM MgCl2, 1mM mercaptoethanol, 0.5mM ZnCl2, 20mM Na cacodylate pH 6.0, mixed with 3vols of well solution (12% PEG6000, 0.3M KCl, 100mM Na cacodylate pH 6.3, 100mM MgSO4, 2mM ZnCl2, 0.1% octyl-glucopyranoside hanging drop vapour diffusion at 20°C 2.2Å Hg MAD and SIR Silvian et al., 1999
1QF6 ThrRS-tRNA complex 12% PEG4000, 0.2M ammonium acetate, 50mM Na-MES pH6.5, ATP hanging drop 2.9Å Hg Sankaranarayanan et al. 1999
1C0A E. coli AspRS-tRNA complex 2M AS, 15% Glycerol, 50mM Bis-Tris propane pH 6.8 hanging drop vapour diffusion at 4°C 2.4Å MR with AMORE Eiler et al. 1999
1QLN T7 RNA pol initiation complex 20% PEG8000, 0.2M AS, 0.25% octyl-glucopyranoside, 0.1M Tris-HCl pH8.7 vapor diffusion 2.4Å Se-Met. Hg, Iodine Cheetham and Steitz, 1999
1DK1

1G1X

 S15/S6/S18-rRNA complex 1.8M AS, 20 mM MgCl2, 50 mM K catodylate pH 6.5 sitting drop vapour diffusion 2.6Å UO2(NO3)2, HgCl2, ethyl-Hg-phosphate, cis and trans Pt(NH3)2Cl2 Agalarov et al., 2000;
1EC6 NovaKH domain-ssRNA complex 25% PEGMME 5000, 150 mM AS, 100mM MES pH 6.5 sitting drop vapour diffusion at 20°C 2.4Å 5-bromouracil Lewis et al., 2000
1DFU E. Coli Ribosomal Protein L25 with a 5S rRNA Fragment 20% MPD, 100mM KCl, 10mM MgCl2, 50mM cacodylate pH 6.0 at 19°C sitting drop vapous diffusion 1.8Å Se-Met Lu and Steitz, 2000
1EFW T. thermophilus Asp-tRNA RS with E. coli tRNA 0.7M Sodium citrate, 10mM MgCl2, 50mM Na-HEPES pH7.5 hanging drop at 17°C 3.0Å MR using free ttAspRS Briand et al. 2000
T. thermophilus ProRS-tRNA(Pro) complex complex in 12% AS, 50mM Tris-malate pH 7.5, 5mM MgCl2, 2mM DTT, 2mM NaN3 equilibrated with 32% AS, 50mM Tris-malate pH 7.5 vapour diffusion at 20°C 2.8Å molecular replacement using free ProRS Yaremchuk et al., 2000a; 2000b
1EIF Phenylalanyl-tRNA Synthetase From Thermus thermophilus Complexed With Cognate tRNA(Phe) AS, Mg-sulphaate pH 7.2 hanging drop 3.3Å Goldgur et al. 1997
1GAX

1IVS

 Thermus Thermophilus Valyl-tRNA Synthetase Complexed With tRNA(Val) 1.0M AS, 50mM sodium cacodylate pH6.5, 10mM Mg(SO4), 6% 1,8-diaminooctane hanging drop 2.9Å K2PtCl4

NaAuCl4

 Fukai et al. 2000

Fukai et al. 2003
1F7U

1F7V

 Arginyl-tRNA Synthetase Complexed With The tRNA(Arg) and L-Arg 2.0 M AS, 5% hexanedial, 100mM Na-cacodylate pH7.5, 20mM MgSO4, 10mM ATP hanging drop 2.2Å MR using free yArgRS Delagoutte et al. 2000
1E7K The spliceosomal 15.5KD protein bound to U4 snRNA fragment 0.1M HEPES pH7.6, 16.5% PEG2000, 30mM MgCl2, 5mM DTT, 0.04% NaN3 hanging drop 2.9Å Se-Met Vidovic et al., 2000
1FXL Hud and Au-Rich Element Of The C-Fos RNA 30% PEG 3350, 10mM CaCl2, 50mM Tris pH8.5, 0.2M NH4Cl hanging drop at 20°C 1.8Å 5-iodouracil at position 6 in cfos-11 RNA Wang and Hall, 2001
1G2E Hud and Au-Rich Element Of The Tumor Necrosis Factor RNA 30% PEG 3350,10mM CaCl2, 50mM Tris pH8.5, 0.2M NH4Cl hanging drop at 20°C 2.3Å MR using cfos RNA Wang and Hall, 2001
1G59 Thermus thermophilus Glutamyl-tRNA Synthetase Complexed With tRNA(Glu) 22% PEG1500, 10mM MgCl2, 37mM MOPS pH6.7, 37mM AS, 5mM mercapto-ethanol, 1% 2-methyl 2,4-pentandiol hanging drop 2.4Å MR using free GluRS Sekine et al., 2001
1DUL

1HQ1

 M domain(Ffh)-4.5S RNA complex 10% isopropanol, 50mM Na-MES pH 5.6, 200 KCl, 12.5mM MgCl2, 35mM C-HEGA 10 sitting drop vapour diffusion 1.8Å

1.5Å

 MAD using selleno-methionine labelled protein Batey et al., 2000

Batey et al., 2001
1E8O SRP9/SRP14/Alu RNA(SA50) complex The SA50 complex containing 2.5mM HEPES pH7.5, 25mM Na acetate pH5.0, 10 mM MgCl2, 75mM NaCl, 210mM AS, 5mM dithiothreitol, 11% PEG2000 was equilibrated over a resovoir solution (50mM Na acetate pH5.0, 10mM MgCl2, 140mM NaCl, 390mM AS, 21% PEG2000) hanging drop vapour diffusion at 12°C 3.2Å Molecular replacement using free protein complex (SRP9/14) Weichenrieder et al., 2000
1E8S SRP9/SRP14/Alu RNA (SA88) complex The SA88 complex in 28mM HEPES pH7.0, 10mM MgCl2, 75mM NaCl, 0.4mM Eu(NO3)3, 5mM DTT, 210mM (NH4)2SO4, 12% PEG400 was equilibrated over a resovoir (50mM HEPES pH7.0, 10mM MgCl2, 150mM NaCl, 0.8mM Eu(NO3)3, 390mM AS, 23% PEG400) haging drop vapour diffusion at 12°C 4Å MAD using Eu L(III) edge Weichenrieder et al., 2000
1F7Y S15-rRNA complex 1M AS, 1.5mM MgCl2, 50mM KCl, 50mM Na cacodylate pH 6.2 vapour diffusion at 20°C 2.8Å SeMet MAD combined with K2PtCl4 and TbCl3 MIR phages Nikulin et al. 2000; Ennifar et al. 2000
1DDL Desmodium Yellow Mottle Tymovirus 2M sodium formate, 0.1M sodium acetate pH4.6 sitting drop 2.7Å MR with X-PLOR Larson et al., 2000
1EFU Ribosomal Protein Tl5, One Of The Ctc Family Proteins, Complexed With A Fragment Of 5S rRNA 7% MPD, 100mM MgCl2, 100mM KCl, 10mM Na-cacodylate pH7.0, 4mM CdCl2, equilibrated with 0.2M KCl, 12% MPD hanging drop 2.3Å MR using E. Coli L25-RNA complex

Se-Met

 Fedorov et al. 2001
1HYS HIV-1 Reverse Transcriptase In Complex With A Polypurine Tract RNA: DNA 29-31% AS, 0.1M cacodylate pH5.6 hanging drop at 4°C 3.0Å MR using WT HIV RT-DNA:DNA-Fab Sarafianos et al. 2001
1I5L Sm-Like Protein (Af-Sm1) From Archaeoglobus Fulgidus Complexed With Short Poly-U RNA 12% PEG6000, 0.1M Na-citrate pH 4.3 hanging drop at 20°C 2.7Å MR using a heptameric ring of PA-Sm1 Toro et al. 2001
1I6H RNA Polymerase II Elongation Complex PEG 6000, 390mM (NH4)2 HPO4/NaH2PO4 pH6.5, 50mM dioxane, 10mM DTT sitting drop in anaerobic condition 3.3Å MR with AMORE Gnatt et al. 2001
1I6U Ribosomal Protein S8/Rrna Complex From Methanococcus Jannaschii 2.8M AS, 100mM Na-cacodylate pH6.5 hanging drop at 4°C 2.6Å Se-Met Tishchenko et al. 2001
1IL2 The E. Coli Aspartyl-tRNA Synthetase: Yeast tRNA(Asp): Aspartyl-Adenylate Complex 1mM MgCl2, 1.5M AS, 75mM Bis-Tris propane pH6.7, 0.5mM AMP-PCP equilibrated with 1.9M AS, 100mM hanging drop 2.6Å MR with ALMN, TSFGEN Moulinier et al. 2001
1H4Q

1H4S

 Prolyl-tRNA Synthetase From Thermus Thermophilus Complexed With Trnapro(Cgg), ATP and Prolinol Classification Aminoacyl-tRNA Synthetase 12% AS, 50mM Tris-malate pH7.5, 5mM MgCl2, 2mM DTT, 2mM NaN3 equilibrated with 32% AS, 50mM Tris-malate pH7.5 hanging drop at 20°C 3.0Å MR with AMORE Yaremchuk et al. 2000
1JBT ibotoxin Restrictocin Complexed With A 29-Mer Sarcin/Ricin Domain RNA Analog 2-10% PEGMME 5000, 0.1M K-MES pH6.5, 50mM KCl micro seeding at 19°C 2.7Å MR using uncomplexed structure Yang et al. 2001
1JBR The Ribotoxin Restrictocin and A 31- Mer Srd RNA Inhibitor 2-10% PEGMME 5000, 0.1M K-MES pH6.5, 50mM KCl micro seeding at 19°C 2.15Å MR using uncomplexed structure Yang et al. 2001
1JID Human Srp19 In Complex With Helix 6 Of Human Srp RNA 0.45M KCl, 5mM MgCl2, 50mM NaCl, 10% PEG 3350, 5% isopropanol, citrate pH5.6 hanging drop at 18°C 1.8Å MR using human helix6 RNA and brominated uridin Wild et al. 2001
1K8W The E. Coli Pseudouridine Synthase Trub Bound To A T Stem-Loop RNA 1.5M AS, 10mM MgCl2, 2& PEG400, 0.1M K-HEPES pH7.5, 1mM TCEP sitting drop 1.85Å Se-Met MAD C. Hoang, A. R. Ferre-D'Amare
1KNZ Recognition Of The Rotavirus Mrna 3' Consensus By An Asymmetric Nsp3 Homodimer 20% PEG 4000, 0.2M AS, 50mM Na-MES pH5.4 hanging drop at 24°C 2.45Å Se-Met Deo et al. 2001
1KOG E. Coli Threonyl-tRNA Synthetase Interacting With The Essential Domain Of Its Mrna Operator 1.9M AS, 10mM MgCl2, Na-cacodylate pH6.6 hanging drop at 4°C 3.5Å MR using coordinates of delta-N ThrRS Toryes-Larios et al. 2002
1KQ2 Staphylococcus aureus Hfq-RNA Complex 10% PEGMME 550, 15mM MgCl2, 50mM, 100mM KCl batch method 2.7Å MR using free Hfq Schumacher et al. 2002
1L9A SRP19 in complex with the S domain of signal recognition particle RNA 12% PEG4000, 110mM TRIS.HCL PH 7.4, 10% Glycerol, 50mM Mg acetate, 100mM ammonium actate 5mM MgCl2 hanging drop 2.9Å SeMet MAD Oubridge et al. 2002
1LNG The Srp19-7S. S SRP RNA complex of M. Jannaschii 18% PEG 3350, 0.1M HEPES pH7.0, 0.1M Mg acetate, 0.02M CaCl2, 5% DMSO hanging drop at 18°C 2.3Å MR (CNS) using helix 6 and 8 structure Haizl et al. 2002
1M5K

1M5P

1M5V

 Hairpin Ribozyme (U1A) 21% MPD, 0.22-0.26M Ammonium Chloride, 20mM CaCl2, PH 5.0 sitting drop at 22°C 2.4Å MAD with SeMet (U1A) Rupert et al. 2002
1M8W

1M8Y

 The Pumilio-homology domain from human Pumilio1 in complex with Nre1-14 RNA 14% PEG 3350, 0.1M Li2SO4, 0.1M Na-citrate pH5.6 hanging drop at 20°C 2.2Å MR with AMORE using free HSPUM-Hb structure Wang et al. 2002
1M8V Pyrococcus abyssii Sm protein in complex with a Uridine heptamer 10% PEG 1000, 0.1M imidazole pH8.0, 0.25M Ca acetate hanging drop at 4°C 2.6Å MR with AMORE Thore et al. 2003
1H2D Ebola virus matrix protein Vp40 N-terminal domain in complex with RNA (Low-resolution Vp40[31-212] variant) 100mM Na-acetate pH4.6, 35 % MPD, 4 % Glycerol hanging drop 2.6Å MR with BEAST and AMORE Gomis-Ruth et al. 2003
1MFQ A ternary S-domain complex of human signal recognition particle 14% (w/v) PEG8000, 0.1M Na-cacodylate pH6.5, 0.4M Li2SO4, 80mM MgCl2 hanging drop 3.1Å Hg SIRS Kuglstatter et al. 2002
1H2U

1H2T

 The human nuclear Cap-binding-complex (Cbc) in complex with a Cap analogue M7Gpppg 10% PEG6000, 100mM MES pH6.0 hanging drop at 4°C 2.4Å

2.1Å

 MR with MOLREP Mazza et al 2002
1H38 T7 RNA polymerase elongation complex 10% PEG 8000, 10% glycerol, 5mM beta-mercaptoethanol, 100mM Tris bufer pH 8.1 sitting drop at 20°C 2.9Å MR using the coordinates of the T7RNAP IC Tahirov et al. 2002
1MJI RNA-protein interactions within thebacterial ribosomal protein L5/5S rRNA complex 50mM Na-cacodylate pH6.5, 0.1M Mg-acetate, 50mM KF, 10% PEG8000, equilibrated with hanging drop at 25°C 2.5Å Se-Met MAD Perederina et al. 2002
1H3E Tyrosyl-tRNA synthetase from Thermus thermophilus complexed with wild-type tRNAtyr(Gua),ATP and tyrosinol 10mM MgCl2, 10mM ATP, 50mM HEPES pH7.0, 0.8M AS equilibrated with 1.5-1.6M AS, 0.1M HEPES pH7.0 hanging drop at 20°C 2.9Å MR using catalytic and a-helical domain of ttTyrRS Yaremchuk et al. 2002
1MSW T7 RNA polymerase elongation complex 6% PEG 8000, 0.2M Li2SO4, 10mM Mg(OAC)2, 50mM Tris-HCl pH 7.0, 5mM DTT hanging drop at 21°C 2.1Å MR using C-terminal domain of T7 RNAP initiation complex Yin and Steitz 2002
1MZP The L1 protuberance in the ribosome 27% PEG400, 50mM Tris-HCl pH 8.5, 100mM KCl, 10mM MgCl2, hanging drop at 5°C 2.65Å Se-Met MAD Nikulin et al.
1N1H

1N38

1N35

 Initiation Complex Of Polymerase 3 From Reovirus 7.5% PEG8000, 150mM NaCl, 0.1M HEPES pH 7.8, 10% glycerol, hanging drop at 22°C 2.8Å EMTS, K2PtCl4, K3UO2F5 Tao et al. 2002
1N77

1N78

 Thermus thermophilus Glutamyl-tRNA synthetase complexed with tRNA(Glu) and ATP 10mM MOPS-Na pH6.5, 5mM MgCl2, 1% PEG6000, 2.5mM mercaptoethanol, 2mM ATP, 2mM glutamate equilibrated with 10% PEG6000 hanging drop at 4 or 20°C 2.4Å MR using free GluRS Sekine et al. 2003
1NB7 Hc-J4 RNA polymerase complexed with short RNA template strand 20% PEG4000, 50mM MES pH5.0, 10% glyserol, 5mM DTT micro-batch at 18°C 2.9Å MR using 1QUV O'Farrell et al. 2003
1J1U Methanococcus jannaschii tyrosyl-tRNA synthetase complexed with tRNA(Tyr) and L-Tyrosine 30% 1,6-hexanedial, 0.2M ammonium chloride, 10mM MgCl2, 1mM Zn-acetate, 50mM Na-acetate pH4.0 hanging drop at 30°C 1.95Å Se-Met SAD `Kobayashi et al. 2003
1NH3 Human Topoisomerase I Ara-C Complex TRIS, MgCl2, PEG 400, DTT, pH 7.7, sitting drop at 276K 3.1Å MR using human Topoisomerase Chrencik et al. 2003
1J2B Archaeosine tRNA-guanine transglycosylase complexed with -form tRNA(Val) 0.1M citrate, 1M NH4H2PO4 pH 5.6, 0.3% xylitol equilibrated with 0.1M citrate pH5.6, 1M NH4H2PO4, 0.4M NaCl hanging drop at 20°C 3.3Å MR using free ArcTGT Ishitani et al. 2003
1O0C

1O0B

 L-Glutamate and AMPCPP bound to Glutamine Aminoacyl tRNA Synthetase 2.25M AS, 20mM Pipes pH 7.00, 10mM MgSO4, 20mM mercaptoethanol, 60mM glutamine equilibrated with 2M AS 20mM Pipes pH7.0, 10mM MgSO4, 20mM mercaptoethanol hanging drop at 25°C 2.7Å MR using GlnRS-tRNAgln Bullock et al.2003
1C04 50S ribosomal subunit PEG 6000, KCl, Ammonium chloride, MgCl2, acetate hanging drop 5.0Å tungsten Ban et al., 2000
1FFK

1JJ2

1K9M

1KQS

1M1K

1M90

 The Large Ribosomal Subunit From Haloarcula Marismortui 1.2M KCl, 0.5M NH4Cl, 0.1M K-acetate, 30mM MgCl2, 7% PEG6000, 15mM Tris, 15mM MES, 1mM CdCl2, pH7.1 hanging drop 2.4Å Os(NH3)5, UO2F5, Ir(NH3)6, Os(NH3)6 Ban et al.,

Klein et al. 2001

Hansen et al. 2002
1DV4

1I97

 30S ribosomal subunit 4.5Å Tocilj et al. 1999

Pioletti et al. 2001
1HRO Initiation Factor If1 Bound To The 30S Ribosomal Subunit 0.25M KCl, 75mM NH4Cl, 25mM MgCl2, 13-17%MPD, 0.1M K-cacodylate pH6.5 vapor diffsion at 4°C 3.2Å 3Å structure of 30S Carter et al.
1GIX

1GIY

 Crystal Structure Of The Ribosome At 5.5 A Resolution 5.5Å Yusapov et al. 2001
1JGQ

1JGP

1JGO

 The Path Of Messenger RNA Through The Ribosome. 5.0Å Yusupova et al.2001
1NKW

1JZY

1K01

1JZZ

1JZX

1K8A

1J5A

1NJP

1NJM

1NWY

1NKW

1NWX

1OND

 Structural Basis For The Interaction Of Antibiotics With The Peptidyl Transferase Center In Eubacteria

The 50S large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (Asm)

 10mM MgCl2, 60mM NH4Cl, 5mM KCl, 10mM HEPES pH7.8, 0.1-1% poly and monovalent alchols vapor diffusion at 18°C 3.1Å

3.5Å

 penta-Ir, W12 (tungsten) Harms et al. 2001

Schulenzen et al. 2001

Bashan et al. 2003
1QDN

1FJG

1HNX

1HNZ

1HNW

1IBK

1IBM

1IBL

1N33

1N36

1N34

1N32

 30S ribosomal subunit 14-16% MPD, 0.2M KCl, 75mM NH4Cl, 15mM MgCl2, 0.1M MES or cacodylate pH6.5 hanging drop at 4°C 3.0Å Tungsten clusters W30, W18, W17, W12-Si, W12, W11 Wimberly et al. 2000; Carter et al. 20000 Brodersen et al. 2000; Ogle et al. 2001; Ogle et al. 2003
Abbreviations: AS, ammonium sulphate; PEG, polyethyleneglycol; PEG-MME, polyethyleneglycol-monomethylether; DTT, dithiothreitol
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