Biological Mass Spectrometry and Proteomics

Mass SpectrometryMass spectrometry based proteomic experiments have become an important tool for studying biological systems. The goal of the Biological Mass Spectrometry and Proteomics Laboratory is to implement state-of-the-art mass spectrometric techniques to provide scientists at the LMB with the capabilities to identify, characterise and quantify proteins present in a particular network, pathway, organelle, cell or tissue.

The facility principally employs electrospray ionisation mass spectrometry as the key technology for both qualitative and quantitative characterisation of proteins. However, the Laboratory has access to several mass spectrometric technologies, including MALDI, thereby allowing investigators to adopt multiple analytical strategies. The basic approach for protein analysis is ‘bottom-up’ proteomics, where proteins are initially enzymatically digested with proteases producing a peptide pool representative of the original proteins.

J Mark Skehel is Head of the Biological Mass Spectrometry and Proteomics Laboratory.