Tethering ribosomes at the ER
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One third of eukaryotic proteins are targeted to the endoplasmic reticulum (ER) for synthesis, folding and secretion. The Miller lab studies the inter-related processes of protein folding and export from the ER via COPII-coated vesicles. We use yeast genetics and biochemistry to identify new components and test their mechanism of action. A recent genetic screen identified several polysome-associated proteins that localize to the ER and thus are likely to mark ribosomes engaged in translation of secretory proteins. We now aim to discover the function of these proteins and understand the specific clients that they might regulate. This project will start with yeast genetic screens and high content imaging approaches, and will expand into cell culture work to identify orthologous pathways in human cells.