![[Amino acid and nucleotide alignments]](/images/ALIGNMENTSbar.GIF)
![[Introduction]](/images/introbar.gif)
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V BASE is a comprehensive directory of all human germline variable region sequences compiled from over a thousand published sequences,
including those in the current releases of the Genbank and EMBL data libraries. The database has been developed over several years at
the MRC Centre for Protein Engineering (Cambridge, UK) as an extension of our work on the sequencing and mapping of human antibody
genes and a user friendly tool for their analysis. V BASE has recently been upgraded (1 December 1997) to include nucleotide alignments
for all functional segments, scale maps of all human V gene loci and a number of other new services. These include a new version of the
DNAPLOT alignment software which enables rearranged genes to be reliably assigned to their closest germline counterparts using the latest
germline sequence data (including data from the recently completed VL and D segment sequencing projects) and tables which denote the
numbers of functional segments (which belong to each V gene family), the numbers of functional segments cut by different restriction
enzymes and PCR primers for amplifying rearranged V genes. In addition, all the sequences in V BASE can now be browsed. All the germline V gene sequences in the database have been carefully checked against their original citation and against their EMBL/Genbank entry. V BASE is now updated regularly to include all the latest germline sequence data and is used by over a thousand immunologists and geneticists worldwide. For further information on the the main services click on the following: amino acid and nucleotide sequence alignments, scale maps of the human immunoglobulin loci or sequence alignment software (DNAPLOT) |
Amino acid and nucleotide sequence alignments have been compiled for all functional V, D and J segments (including all the mapped leader sequences). These can be used to design family or segment specific PCR primers and/or oligonucleotide probes. For some segments there are multiple alleles. In these cases, positions with allelic differences are underlined and the specific changes are shown beneath the alignments. For all sequences, links to a master reference list are provided. The following example is an alignment of all the functional VKI segments taken from V BASE:
L1 L2 L3
------------- --- -----
FR1 CDR1 FR2 CDR2 FR3 CDR3
----------------------- ----------------- --------------- ------- -------------------------------- -------
1 2 3 4 5 6 7 8 9
L1-L2-L3 Locus 12345678901234567890123 45678901abcdef234 567890123456789 0123456 78901234567890123456789012345678 9012345
VKI 2-1-(1) O12 DIQMTQSPSSLSASVGDRVTITC RASQSISS------YLN WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQSYSTP
2-1-(1) O2 DIQMTQSPSSLSASVGDRVTITC RASQSISS------YLN WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQSYSTP
2-1-(1) O18 DIQMTQSPSSLSASVGDRVTITC QASQDISN------YLN WYQQKPGKAPKLLIY DASNLET GVPSRFSGSGSGTDFTFTISSLQPEDIATYYC QQYDNLP
2-1-(1) O8 DIQMTQSPSSLSASVGDRVTITC QASQDISN------YLN WYQQKPGKAPKLLIY DASNLET GVPSRFSGSGSGTDFTFTISSLQPEDIATYYC QQYDNLP
2-1-(U) A20 DIQMTQSPSSLSASVGDRVTITC RASQGISN------YLA WYQQKPGKVPKLLIY AASTLQS GVPSRFSGSGSGTDFTLTISSLQPEDVATYYC QKYNSAP
2-1-(1) A30 DIQMTQSPSSLSASVGDRVTITC RASQGIRN------DLG WYQQKPGKAPKRLIY AASSLQS GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC LQHNSYP
2-1-(1) L14 NIQMTQSPSAMSASVGDRVTITC RARQGISN------YLA WFQQKPGKVPKHLIY AASSLQS GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC LQHNSYP
2-1-(1) L1 DIQMTQSPSSLSASVGDRVTITC RASQGISN------YLA WFQQKPGKAPKSLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQYNSYP
2-1-(1) L15 DIQMTQSPSSLSASVGDRVTITC RASQGISS------WLA WYQQKPEKAPKSLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQYNSYP
2-1-(1) L4 AIQLTQSPSSLSASVGDRVTITC RASQGISS------ALA WYQQKPGKAPKLLIY DASSLES GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQFNSYP
2-1-(1) L18 AIQLTQSPSSLSASVGDRVTITC RASQGISS------ALA WYQQKPGKAPKLLIY DASSLES GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQFNSYP
2-1-(1) L5 DIQMTQSPSSVSASVGDRVTITC RASQGISS------WLA WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQANSFP
2-1-(1) L19 DIQMTQSPSSVSASVGDRVTITC RASQGISS------WLA WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC QQANSFP
2-1-(1) L8 DIQLTQSPSFLSASVGDRVTITC RASQGISS------YLA WYQQKPGKAPKLLIY AASTLQS GVPSRFSGSGSGTEFTLTISSLQPEDFATYYC QQLNSYP
2-1-(1) L23 AIRMTQSPFSLSASVGDRVTITC WASQGISS------YLA WYQQKPAKAPKLFIY YASSLQS GVPSRFSGSGSGTDYTLTISSLQPEDFATYYC QQYYSTP
2-1-(1) L9 AIRMTQSPSSFSASTGDRVTITC RASQGISS------YLA WYQQKPGKAPKLLIY AASTLQS GVPSRFSGSGSGTDFTLTISCLQSEDFATYYC QQYYSYP
U-1-(1) L24 VIWMTQSPSLLSASTGDRVTISC RMSQGISS------YLA WYQQKPGKAPELLIY AASTLQS GVPSRFSGSGSGTDFTLTISCLQSEDFATYYC QQYYSFP
2-1-(1) L11 AIQMTQSPSSLSASVGDRVTITC RASQGIRN------DLG WYQQKPGKAPKLLIY AASSLQS GVPSRFSGSGSGTDFTLTISSLQPEDFATYYC LQDYNYP
2-1-(U) L12 DIQMTQSPSTLSASVGDRVTITC RASQSISS------WLA WYQQKPGKAPKLLIY DASSLES GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC QQYNSYS
L1
---------------------------
CDR1
-------------------------------------------
VKI 10 20 30 40
| | | |
D I Q M T Q S P S S L S A S V G D R V T I T C R A S Q S I S S Y L N W Y Q Q K P G K A P K L L I Y
O12 GAC ATC CAG ATG ACC CAG TCT CCA TCC TCC CTG TCT GCA TCT GTA GGA GAC AGA GTC ACC ATC ACT TGC CGG GCA AGT CAG AGC ATT AGC AGC TAT TTA AAT TGG TAT CAG CAG AAA CCA GGG AAA GCC CCT AAG CTC CTG ATC TAT
O2 ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...
O18 ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... .A. ..G ... ... GA. ... ... .A. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..C
O8 ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... .A. ..G ... ... GA. ... ... .A. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..C
A20 ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..G ... ... G.. ... ... .AT ... ... GCC ... ... ... ... ... ... ... ... .TT ... ... ... ... ... ...
A30 ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... G.. ... ..A .AT G.. ... GGC ... ... ... ... ... ... ... ... ... ... ... .G. ... ... ...
L14 A.. ... ... ... ... ... ... ... ..T G.. A.. ... ... ... ... ... ... ... ... ... ... ... ..T ... ..G ..G ... G.. ... ... .AT ... ... GCC ... .T. ... ... ... ... ... ... .T. ... ... .A. ... ... ...
L1 ... ... ... ... ... ... ... ... ... ..A ... ... ... ... ... ... ... ... ... ... ... ... ..T ... ..G ... ... G.. ... ... .AT ... ... GCC ... .T. ... ... ... ... ... ... ... ... ... TC. ... ... ...
L15 ... ... ... ... ... ... ... ... ... ..A ... ... ... ... ... ... ... ... ... ... ... ... ..T ... ..G ... ... G.T ... ... ... .GG ... GCC ... ... ... ... ... ... .A. ... ... ... ... TC. ... ... ...
L4 .C. ... ... T.. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... G.. ... ... ..T GC. ... GCC ... ... ... ... ... ... ... ... ..T ... ... ... ... ... ...
L18 .C. ... ... T.. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... G.. ... ... ..T GC. ... GCC ... ... ... ... ... ... ... ... ..T ... ... ... ... ... ...
L5 ... ... ... ... ... ... ... ... ..T ... G.. ... ... ... ... ... ... ... ... ... ... ... ..T ... ..G ... ... G.T ... ... ... .GG ... GCC ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...
L19 ... ... ... ... ... ... ... ... ..T ..T G.. ... ... ... ... ... ... ... ... ... ... ... ..T ... ..G ... ... G.T ... ... ... .GG ... GCC ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...
L8 ... ... ... T.. ... ... ... ... ... .T. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... G.. ... ... ..T ... ... GCC ... ... ... ..A ... ... ... ... ... ... ... ... ... ... ...
L23 .C. ... .G. ... ... ... ... ... .T. ... ... ... ... ... ... ... ... ... ... ... ... ... ... T.. ..C ... ... G.. ... ... ..T ... ... GCC ... ... ... ..A ... ... .CA ... ... ... ... ... T.C ... ...
L9 .C. ... .G. ... ... ... ... ... ... ..A T.C ... ... ... AC. ... ... ... ... ... ... ... ..T ... ..G ... ... G.T ... ... ..T ... ... GCC ... ... ... ..A ... ... ... ... ... ... ... ... ... ... ...
L24 .T. ... TG. ... ... ... ... ... ... .TA ..C ... ... ... AC. ... ... ... ... ... ... .G. ..T ... ATG ... ... G.. ... ... ..T ... ... GCC ... ... ... ..A ... ... ... ... ... ... G.. ... ... ... ...
L11 .C. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... G.. ... ..A .AT G.. ... GGC ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...
L12 ... ... ... ... ... ... ... ..T ... A.. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ..T ... ..T ... .GG ..G GCC ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...
L2 L3
----------- ----------------------
CDR2 CDR3
--------------------------- ------------------------------
50 60 70 80 90
| | | | |
A A S S L Q S G V P S R F S G S G S G T D F T L T I S S L Q P E D F A T Y Y C Q Q S Y S T P
O12 GCT GCA TCC AGT TTG CAA AGT GGG GTC CCA TCA AGG TTC AGT GGC AGT GGA TCT GGG ACA GAT TTC ACT CTC ACC ATC AGC AGT CTG CAA CCT GAA GAT TTT GCA ACT TAC TAC TGT CAA CAG AGT TAC AGT ACC CCT CC
O2 ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..
O18 .A. ... ... .A. ... G.. .CA ... ... ... ... ... ... ... ..A ... ... ... ... ... ... ..T ... T.. ... ... ... ..C ... ..G ... ... ... A.. ... ..A ..T ... ... ... ... TA. G.T .A. CT. ... ..
O8 .A. ... ... .A. ... G.. .CA ... ... ... ... ... ... ... ..A ... ... ... ... ... ... ..T ... T.. ... ... ... ..C ... ..G ... ... ... A.. ... ..A ..T ... ... ... ... TA. G.T .A. CT. ... ..
A20 ... ... ... .C. ... ... TCA ... ... ... ..T C.. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... G.. ... ... ..T ... ... ... A.. TA. A.. ... G.. ... ..
A30 ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ..A ... ... ... ..A ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... .T. ... CA. A.T ... TA. ... ..
L14 ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ..A ... ... ... ..A ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... .T. ... CA. A.T ... TA. ... ..
L1 ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ..C ... ... TA. A.T ... TA. ... ..
L15 ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ..C ... ... TA. A.T ... TA. ... ..
L4 .A. ..C ... ... ... G.. ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... ... ... TT. A.T ... TA. ... .A
L18 .A. ..C ... ... ... G.. ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... ... ... TT. A.T ... TA. ... .A
L5 ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ... ..T ... ... ... GC. A.. ... TT. ... ..
L19 ... ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ..T ... ... ..C ... ..G ... ... ... ... ... ... ... ..T ... ... ... GC. A.. ... TT. ... ..
L8 ... ... ... .C. ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ..A ... ... ... ..A ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... ... ... CT. A.T ... TA. ... ..
L23 TA. ... ... ... ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ..G ... .A. ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... ... ... TA. ..T ... ... ... ..
L9 ... ... ... .C. ... ... ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ... ... ... ... ... ... ... T.C ... ..G T.. ... ... ... ... ... ..T ... ... ... ... TA. ..T ... TA. ... ..
L24 ... ... ... .C. ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ..T T.C ... ..G T.. ... ... ... ... ... ..T ... ... ... ... TA. ..T ... TT. ... ..
L11 ... ... ... ... ..A ... ... ... ... ... ... ... ... ..C ... ... ... ... ..C ... ... ... ... ... ... ... ... ..C ... ..G ... ... ... ... ... ... ..T ... ... .T. ..A GA. ... .A. TA. ... ..
L12 .A. ..C ... ... ... G.. ... ... ... ... ... ... ... ..C ... ... ... ... ... ... ..A ... ... ... ... ... ... ..C ... ..G ... ..T ... ... ... ... ..T ... ..C ... ... TA. A.T ... TAT T.. ..
Segment Sequences (ref) Nucleotide (amino acid) changes O12 DPK9(94)/O12(88) V3b(88) 10 TCC>TTC (S>F), 86 TAC>TAT, 89 CAA>CAG, 90 CAG>TGT (Q>C), 91 AGT>GGT (S>G), 94 ACC>ACA O2 DPK9(94)/O2(88) O18 DPK1(94)/O18(89)/O18a(89) O8 DPK1(94)/O8(89) A20 DPK4(94)/A20(90) A30 A30(92) L14 DPK2(94)/L14(92) L1 HK137(68) L15 DPK7(94)/HK134(68)/HK166(68) HK101(67)/HK146(68)/HK189(68) 26 AGT>AGG (S>R) L4 Va'(71) DPK31(94)/Va(71) 35 TGC>TGA (W>*), 93 AGT>AAT (S>N) L18 Va'(71) Va''(73) 93 AGT>AAT (S>N) L5 DPK5(94)/Vb(71) Vb'(71) 96 CC>TC L19 DPK6(94)/Vb''(73) Vb'(71) 10 TCT>TCC, 74 ACT>ACC 96 CC>TC L8 DPK8(94)/Vd(71) L23 L23(92)/L23a(92) L9 Ve(71) L24 DPK10(94)/L24a(92)/V13(69) L11 DPK3(94)/L11(89) L12 HK102(67)/V1(69) L12a(92) 50 GAT>AAG (D>K), 51 GCC>GCG, 52 TCC>TCT, 54 TTG>TTA
![[D map]](DMAP.GIF)
Simply paste the rearranged sequence into the box and hit 'Start'. Your sequence will be returned aligned to the closest VH, D and JH segments if it was a VH gene, the closest VK and JK segments if it was a VK gene or the closest VL and JL segments if it was a VL gene. For the V segments the top five alignments are given and for the D and J segments the top three alignments are given (where there are identical scores, the germline sequences are ranked as to whether they have been confirmed by independently laboratories and/or whether they have been mapped).
For each germline sequence a link to the EMBL database and the map position (if known) is provided. The alignment can be returned numbered and padded according to the Kabat or Chothia nomenclatures and the software will tell you whether the rearrangement was in or out of frame. In addition, the VH, VK or VL segment family and the reading frame of the D segment (if a VH gene) are provided. The following is an example of a VH gene aligned to V BASE using this DNAPLOT interface:
Sequence to be aligned:
GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCAGGGCGGTCCCTGAGACT CTCCTGTACAGCTTCTGGATTCACCTTTGGTGATTATGCTATGAGCTGGGTCCGCCAGC CTCCAGGGAAGGGGCTGGAGTGGGTAGGTTTCATTAGAAGCAAAGCTTATGGTGGGACA ACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCATCTCAAGAGATGATTCCAAAAG CATCGCCTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTGTATTACTGTA CTAGAGTAGGGTATTATGATTACGTTTGGGGGAGTTATCGTTATACCGGAACGTACTAC TTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA
EMBL Locus Name score GAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTACAGCCAGGGCGGTCCCTGAGACTCTCCTGTACAGCTTCTGGATTCACCTTTGGTGAT______TATGCTATGAGCTGGGTCCGCCAGCCTCCAGGGAAGGGGCTGGAGTGGGTAGGTTTCATTAGAAGCAAAGCTTATGGTGGGACAACAGAATACGCCGCGTCTGTGAAAGGCAGATTCACCATCTCAAGAGATGATTCCAAAAGCATCGCCTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTGTATTACTGTACTAGAGTAGGGTATTATGATTACGTTTGGGGGAGTTATCGTTATACCGGAACGTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA M99676 3-49 V3-49+ 1419 .............................................................................................______...............T........G....................................................................A.......................................G..........................................................................A M99401 - LSG12.1 1410 ..............................................C..........................................GT..______...C....................G.......................................................................................................................................................................................A Z12357 3-22 DP-57/hv3003...+ 1230 .........................................T...G....................G....C..............CA..T.C______..CTAC......G...........G....C..............A...................A.......A...................GA..A......................A...........................A................G....................G...............T.C....A Z12336 3-g DP-34/DA-10+ 1230 ....................C..............C.....T...G....................G....C..............CA....C______..CTAC..................G....C..................................A.......A...................GA..A......................A...........................A........................G.G..........G...............G.G....A M99659 3-22 V3-22P+ 1212 ........T...................C............T...G....................G....C..............CA..T.C______..CTAC......G...........G....C..............A...................A.......A...................GA..A......................A...........................A................G....................G...............T.C....AYour sequence uses a V segment of the VH3 family.
EMBL Locus Name score TGTACTAGAGTAGGGTATTATGATTACGTTTGGGGGAGTTATCGTTATACCGGAACGTACTACTTT X97051 3-16 D3-16 185 ______________..................................... X97051 3-10 D3-10/DXP'1 65 _________________G...T.C..T.G..C..........TA.A.C X97051 3-03 D3-3/DXP4 65 ____________________GT......A..TTT.....GG.TA.......The D segment is used in reading frame 2
EMBL Name score TGTACTAGAGTAGGGTATTATGATTACGTTTGGGGGAGTTATCGTTATACCGGAACGTACTACTTTGACCACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA X86355 JH4b 226 __________________________________________________________...........T...................................G J00256 JH4a 217 __________________________________________________________...........T..........A........................G M25625 JH4d 199 __________________________________________________________G..........T..........A..G.....................G
CTRVGYYDYVWGSYRYTGTYYFIn frame rearrangement
![[Maps]](MAPSbar.GIF)
![[Alignment software]](DNAPLOTbar.GIF)
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21
EMBL Locus Name GAG GTG CAG CTG GTG GAG TCT GGG GGA GGC TTG GTA CAG CCA GGG CGG TCC CTG AGA CTC TCC
M99676 3-49 V3-49+ --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ---
M99401 - LSG12.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- -C- --- --- --- --- ---
Z12357 3-22 DP-57/hv3003...+ --- --- --- --- --- --- --- --- --- --- --- --- --- --T --- G-- --- --- --- --- ---
Z12336 3-g DP-34/DA-10+ --- --- --- --- --- --- --C --- --- --- --- --C --- --T --- G-- --- --- --- --- ---
M99659 3-22 V3-22P+ --- --- --T --- --- --- --- --- --- -C- --- --- --- --T --- G-- --- --- --- --- ---
_________________H1________________
____________CDR1___________
22 23 24 25 26 27 28 29 30 31 31a 31b 32 33 34 35 36 37 38 39 40
EMBL Locus Name TGT ACA GCT TCT GGA TTC ACC TTT GGT GAT ... ... TAT GCT ATG AGC TGG GTC CGC CAG CCT
M99676 3-49 V3-49+ --- --- --- --- --- --- --- --- --- --- ... ... --- --- --- --- --- T-- --- --- G--
M99401 - LSG12.1 --- --- --- --- --- --- --- --- --G T-- ... ... --- C-- --- --- --- --- --- --- G--
Z12357 3-22 DP-57/hv3003...+ --- G-- --C --- --- --- --- --C A-- T-C ... ... --C TAC --- --- G-- --- --- --- G--
Z12336 3-g DP-34/DA-10+ --- G-- --C --- --- --- --- --C A-- --C ... ... --C TAC --- --- --- --- --- --- G--
M99659 3-22 V3-22P+ --- G-- --C --- --- --- --- --C A-- T-C ... ... --C TAC --- --- G-- --- --- --- G--
_________________H2____________
________________________________________CDR2____
41 42 43 44 45 46 47 48 49 50 51 52 52a 52b 52c 53 54 55 56 57 58
EMBL Locus Name CCA GGG AAG GGG CTG GAG TGG GTA GGT TTC ATT AGA AGC AAA GCT TAT GGT GGG ACA ACA GAA
M99676 3-49 V3-49+ --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ---
M99401 - LSG12.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ---
Z12357 3-22 DP-57/hv3003...+ --C --- --- --- --- --A --- --- --- --- --- --- -A- --- --- A-- --- --- --- --- ---
Z12336 3-g DP-34/DA-10+ --C --- --- --- --- --- --- --- --- --- --- --- -A- --- --- A-- --- --- --- --- ---
M99659 3-22 V3-22P+ --C --- --- --- --- --A --- --- --- --- --- --- -A- --- --- A-- --- --- --- --- ---
___________________________
59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79
EMBL Locus Name TAC GCC GCG TCT GTG AAA GGC AGA TTC ACC ATC TCA AGA GAT GAT TCC AAA AGC ATC GCC TAT
M99676 3-49 V3-49+ --- A-- --- --- --- --- --- --- --- --- --- --- --- --- -G- --- --- --- --- --- ---
M99401 - LSG12.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ---
Z12357 3-22 DP-57/hv3003...+ --G A-- A-- --- --- --- --- --- --- --A --- --- --- --- --- --- --- --- --- A-- ---
Z12336 3-g DP-34/DA-10+ --G A-- A-- --- --- --- --- --- --- --A --- --- --- --- --- --- --- --- --- A-- ---
M99659 3-22 V3-22P+ --G A-- A-- --- --- --- --- --- --- --A --- --- --- --- --- --- --- --- --- A-- ---
80 81 82 82a 82b 82c 83 84 85 86 87 88 89 90 91 92 93 94
EMBL Locus Name CTG CAA ATG AAC AGC CTG AAA ACC GAG GAC ACA GCC GTG TAT TAC TGT ACT AGA
M99676 3-49 V3-49+ --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ---
M99401 - LSG12.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- ---
Z12357 3-22 DP-57/hv3003...+ --- --- --- --G --- --- --- --- --- --- --G --- --- --- --- --- T-C ---
Z12336 3-g DP-34/DA-10+ --- --- --- --- --- --- -G- G-- --- --- --G --- --- --- --- --- G-G ---
M99659 3-22 V3-22P+ --- --- --- --G --- --- --- --- --- --- --G --- --- --- --- --- T-C ---