Mark van Breugel
Structure and assembly mechanisms of centrioles
Centrioles are large and complex cell organelles that form the core of centrosomes and are essential for templating cilia and flagella. These structures are important for many cellular functions like cell division, fluid movement, motility, and sensing. Thus, it is not surprising that centriole dysfunction is associated with many human diseases such as microcephaly, ciliopathies and also cancer and infertility.
In the past many centriole components have been identified by genetic, cell biological and biochemical methods, but how they come together to make functional centrioles is an unresolved question. The architecture of centrioles has so far been studied by low-resolution methods. These showed centrioles to be 9-fold symmetric, barrel-shaped structures with the centriole wall built of microtubule triplets (Figure 1). My lab aims to elucidate how the centriolar components build-up centrioles and what precise role these components have in proper centriole functioning.
Using a combination of cell biology, biochemistry and X-ray crystallography, we recently showed how a critical assembly intermediate of centrioles is organised and how this contributes to the establishment of the 9-fold symmetry of centrioles (Figure 1). Our goal is now to understand how the centriolar assembly is extended from this intermediate and what the other important centriolar components do in this process.
- Joseph N, Al-Jassar C, Johnson CM, Andreeva A, Barnabas DD, Freund SMV, Gergely F, van Breugel M. (2018)
Disease-Associated Mutations in CEP120 Destabilize the Protein and Impair Ciliogenesis
Cell Rep. 23(9): 2805-2818. doi: 10.1016/j.celrep.2018.04.100.
- Liu Y, Gupta GD, Barnabas DD, Agircan FG, Mehmood S, Wu D, Coyaud E, Johnson CM, McLaughlin SH, Andreeva A, Freund SMV, Robinson CV, Cheung SWT, Raught B, Pelletier L, van Breugel M. (2018)
Direct binding of CEP85 to STIL ensures robust PLK4 activation and efficient centriole assembly
Nat Commun. 9(1): 1731. doi: 10.1038/s41467-018-04122-x.
- Al-Jassar C, Andreeva A, Barnabas DD, McLaughlin SH, Johnson CM, Yu M, van Breugel M. (2017)
The Ciliopathy-Associated Cep104 Protein Interacts with Tubulin and Nek1 Kinase
Structure 25(1): 146-156. doi: 10.1016/j.str.2016.11.014. PMID: 28017521
- van Breugel, M., Wilcken, R., McLaughlin, S.H., Rutherford, T.J. and Johnson, C.M. (2014)
Structure of the SAS-6 cartwheel hub from Leishmania major
eLife 3: e01812.
- Cottee, M.A., Muschalik, N., Wong, Y.L., Johnson, C.M., Johnson, S., Andreeva, A., Oegema, K., Lea, S.M., Raff, J.W. and van Breugel, M. (2013)
Crystal structures of the CPAP/STIL complex reveal its role in centriole assembly and human microcephaly
eLife 2: e01071.
- van Breugel, M., Hirono, M., Andreeva, A., Yanagisawa, H.A., Yamaguchi, S., Nakazawa, Y., Morgner, N., Petrovich, M., Ebong, I.O., Robinson, C.V., Johnson, C.M., Veprintsev, D. and Zuber, B. (2011)
Structures of SAS-6 suggest its organization in centrioles
Science 331: 1196-1199
- Deepak Barnabas
- Ivan Rosa e Silva
- Marike Worrall (nee van Roon)