Our setup provides facilities for:
- growing safety level 1 organisms
in volumes between 40-120 L
- in-place sterilisation of media
- loops/sensors for DO (dissolved oxygen), OD600, temp, agitation,
pH
- harvesting using continuous centrifuge (120 L in 1 h)
- cell disruption using French press at pressures up to 40 kPSI,
170 ml/min
- type 19 rotors for clarification of 2 L at 19.000 rpm
Before using this equipment (excluding the disruptor, which is free to anyone) you must see Jo Butler (x2296, email pjgb) to enable card access to the basement fermentor room.
Due to the complex nature of the equipment and some safety concerns, it is mandatory that the first run is supervised by an experienced user.
