{"id":8,"date":"2022-02-14T17:14:40","date_gmt":"2022-02-14T17:14:40","guid":{"rendered":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/?page_id=8"},"modified":"2025-10-08T10:07:01","modified_gmt":"2025-10-08T10:07:01","slug":"methods","status":"publish","type":"page","link":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/methods\/","title":{"rendered":"Methods"},"content":{"rendered":"\n<p class=\"has-large-font-size\" id=\"our-interest-is-in-developing-methods-to-understand-cellular-organization-in-four-dimensions-in-particular-we-develop-and-use-structural-methods-to-investigate-cell-and-developmental-biology-phenomena-also-linked-to-several-disease-phenotypes\">Our interest is in developing new tools to understand nuclear-cytoskeletal organisation in four dimensions and enable experiments and data analysis which are impossible with existing technologies. With the support of the mechanical and electronic workshop and of collaborators, we also aim to couple cell biology and structural techniques and integrate data at different scales and resolutions to study nuclear remodelling processes. Here the main methods used in the lab:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Electron cryo Tomography \/ Electron cryo microscopy<\/li>\n\n\n\n<li>Correlative Light and Electron Microscopy<\/li>\n\n\n\n<li>Light microscopy and cryo light microscopy<\/li>\n\n\n\n<li>Integrative structural biology<\/li>\n\n\n\n<li>Biochemistry methods<\/li>\n\n\n\n<li>Cell biology methods<\/li>\n\n\n\n<li>Custom grid design<\/li>\n\n\n\n<li>Molecular modelling<\/li>\n\n\n\n<li>Software development using AI<\/li>\n\n\n\n<li>Biophysical methods<\/li>\n<\/ul>\n\n\n\n<div class=\"wp-block-columns are-vertically-aligned-center is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"205\" height=\"221\" data-id=\"272\" src=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/lamellae_mapping_at_low_magnification-1-e1648017303124.png\" alt=\"\" class=\"wp-image-272\" \/><figcaption class=\"wp-element-caption\">cryo-lamellae on an electron microscopy grid<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" data-id=\"271\" src=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-1024x683.png\" alt=\"\" class=\"wp-image-271\" srcset=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-1024x683.png 1024w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-300x200.png 300w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-768x512.png 768w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-1536x1025.png 1536w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-2048x1366.png 2048w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/Matteo_at_the_microscope_Andreas_Henn-600x400.png 600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">microscope alignment<\/figcaption><\/figure>\n<\/figure>\n<\/div>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-full is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"772\" height=\"708\" data-id=\"53\" src=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/02\/nuclearaberrat-e1653314337434.png\" alt=\"\" class=\"wp-image-53\" srcset=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/02\/nuclearaberrat-e1653314337434.png 772w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/02\/nuclearaberrat-e1653314337434-300x275.png 300w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/02\/nuclearaberrat-e1653314337434-768x704.png 768w\" sizes=\"auto, (max-width: 772px) 100vw, 772px\" \/><figcaption class=\"wp-element-caption\">cryo tomographic slice of a nuclear bleb<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"699\" height=\"596\" data-id=\"259\" src=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/overlaid_fluorescent_spot_on_cryo_lamella-e1653314308593.png\" alt=\"\" class=\"wp-image-259\" srcset=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/overlaid_fluorescent_spot_on_cryo_lamella-e1653314308593.png 699w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/03\/overlaid_fluorescent_spot_on_cryo_lamella-e1653314308593-300x256.png 300w\" sizes=\"auto, (max-width: 699px) 100vw, 699px\" \/><figcaption class=\"wp-element-caption\">Overlayed fluorescent signal on  cryo lamella<\/figcaption><\/figure>\n<\/figure>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1021\" src=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1-1024x1021.jpg\" alt=\"\" class=\"wp-image-567\" srcset=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1-1024x1021.jpg 1024w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1-300x300.jpg 300w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1-150x150.jpg 150w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1-768x766.jpg 768w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1-75x75.jpg 75w, https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-content\/uploads\/sites\/33\/2022\/11\/lax_01963_elife-01963-fig4-v1.tif-1.jpg 1500w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">Atomic resolution cryo-EM map of  the Frh hydrogenase<\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Our interest is in developing new tools to understand nuclear-cytoskeletal organisation in four dimensions and enable experiments and data analysis which are impossible with existing technologies. With the support of the mechanical and electronic workshop and of collaborators, we also aim to couple cell biology and structural techniques and integrate data at different scales and [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[],"class_list":{"0":"post-8","1":"page","2":"type-page","3":"status-publish","5":"entry"},"featured_image_src":null,"featured_image_src_square":null,"_links":{"self":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/pages\/8","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/comments?post=8"}],"version-history":[{"count":26,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/pages\/8\/revisions"}],"predecessor-version":[{"id":889,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/pages\/8\/revisions\/889"}],"wp:attachment":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/media?parent=8"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/allegretti\/wp-json\/wp\/v2\/categories?post=8"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}