{"id":24,"date":"2019-03-25T10:26:26","date_gmt":"2019-03-25T10:26:26","guid":{"rendered":"http:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/bienz\/?page_id=24"},"modified":"2026-03-04T10:45:21","modified_gmt":"2026-03-04T10:45:21","slug":"the-dishevelled-paradigm-assembling-the-wnt-signalosome","status":"publish","type":"page","link":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/bienz\/research-past-and-present\/the-dishevelled-paradigm-assembling-the-wnt-signalosome\/","title":{"rendered":"Wnt signalosome assembly by dynamic head-to-tail polymerisation of Dishevelled\u00a0"},"content":{"rendered":"\n

Dishevelled was known to be associated with the plasma membrane, transducing Wnt signals to all known Wnt effectors. This involves Dishevelled pivoting between Armadillo\/beta-catenin and non-canonical signalling branches including those determining planar polarity. Using confocal microscopy, we discovered an essential function of Dishevelled in translocating the Axin degradasome from the cytoplasm to the cell surface near Wingless signalling sources in the epidermis of Drosophila <\/em>embryos. Glycogen synthase kinase 3 (GSK3) is swept along as an Axin-associated component of the degradasome and reinforces this translocation (via phosphorylating specific motifs in the intracellular tail of the LRP5\/6 co-receptors, as shown by Xi He and colleagues). A series of subsequent experiments led to the concept of the Wnt signalosome, a dynamic signalling platform assembled at the Wnt receptor complex. This involves dynamic head-to-tail polymerisation of its Dishevelled hub, which thus attains a high binding avidity for low-affinity Wnt signalling effectors such as Axin itself. <\/p>\n\n\n\n

All three vertebrate Dishevelled orthologues (DVL1-3) form cytoplasmic puncta upon overexpression in various mammalian cells and tissues, thereby triggering Wnt-independent beta-catenin signalling. These puncta turned out to be highly dynamic protein assemblies without membrane components, and Fluorescence Recovery After Photobleaching (FRAP) experiments of individual DVL2-GFP puncta revealed that the punctate protein exchanges rapidly (half-time ~15 seconds) with the soluble monomeric pool of DVL2-GFP. If observed over 2-3 days, DVL2-GFP puncta grow and coalesce into huge blobs resembling \u2018lipid droplets\u2019 which eventually cause cell death, suggesting that the cellular levels of Dishevelled are limited normally by protein turnover. <\/p>\n\n\n\n

Importantly, we identified point mutations (M2-4) in the DIX domain of DVL2 that are required for signalosome assembly and signalling activity in vivo<\/em> and, crucially, for self-association of purified DIX domain in vitro<\/em>. These mutations allowed us to discover that the DIX domain undergoes dynamic head-to-tail polymerisation. Short reversible DIX domain oligomers can be detected by equilibrium ultracentrifugation, and DIX domain filaments can also be visualised by electron microscopy. Furthermore, purified DIX domain formed helical filaments upon crystallisation, and its structure revealed that the residues mutated in the signalling-defective M2-4 mutants are located in the DIX-DIX interface and engage in key DIX-DIX interactions. We concluded that Dishevelled is a hub protein that assembles a Wnt signalosome by head-to-tail polymerisation via its DIX domain to increasing the local concentration of Dishevelled effector binding sites. Consequently, polymerised Dishevelled attains a high binding avidity for low-affinity effectors such as Axin (the scaffold of the beta-catenin destruction complex, also known as \u2018degradasome\u2019), which it cannot bind at its normal physiological (sub-micromolar) cellular concentration. <\/p>\n\n\n\n

It was known from Feng Cong\u2019s work that Dishevelled binds constitutively to the intracellular face of the Frizzled receptor and mediates its turnover in the absence of Wnt. Binding of Wnt to the extracellular face of Frizzled triggers polymerisation of Dishevelled via its DIX domain, and co-polymerisation between this domain with that of Axin (called DAX) via a heterotypic DIX-DAX interaction. Thus, Dishevelled recruits the Axin degradasome to the Wnt receptor complex at the plasma membrane, which comprises Frizzled and its co-receptor LRP5\/6 (which also binds to some of the Wnts). Furthermore, polymerised Dishevelled recruits casein kinase 1 (CK1) via its PDZ domain, thereby promoting the phosphorylation of specific motifs in the cytoplasmic tail of LRP5\/6. Consequently, these phospho-motifs bind to the catalytic pocket of signalosome-associated GSK3 as competitive inhibitors (according to work by the Weis group), blocking its activity in the degradasome towards beta-catenin. This allows beta-catenin to accumulate, and to be captured in the nucleus by the BCL9 scaffold of the Wnt enhanceosome and loaded onto TCF\/LEF, which in turns relieves the Groucho\/TLE-dependent repression of linked Wnt target genes. <\/p>\n\n\n\n

Signalosomes and degradasomes are three-dimensional molecular condensates, and so their constituent DIX and DAX polymers need to be cross-linked. In the Axin degradasome, APC and beta-catenin act as a cross-linkers of DAX filaments, and thus have essential functions in nucleating degradasome assembly. Furthermore, APC also prevents Axin from binding to Dishevelled at the plasma membrane in fly imaginal disc cells that are not exposed to Wingless stimulation \u2013 likely a mechanism to prevent inadvertent Wingless signal transduction.<\/p>\n\n\n\n

Cross-linking of DIX filaments in Wnt signalosomes is achieved by DEP-dependent dimerisation, which involves DEP domain swapping. This process blocks binding of Dishevelled to the Frizzled receptor, by masking the Frizzled-binding site located in an exposed loop of the monomeric DEP domain. This implies that the signalosomes gradually detach from the plasma membrane during chronic stimulation of cells by canonical Wnt signals. Therefore, DEP domain swapping must be attenuated during non-canonical Wnt signalling when the signalosomes need to be stably associated with Frizzled to orient the actin skeleton of incipient wing hairs, and other planar polarity signalling outputs.<\/p>\n\n\n\n

One of factors limiting the cellular Dishevelled levels is Sudx\/WWP2, a HECT E3 ubiquitin ligase that binds to Dishevelled and whose ligase activity towards Dishevelled is dis-inhibited by Dishevelled polymerisation. The latter also unlocks its ligase activity towards Notch, a functionally relevant substrate in fly imaginal discs and in ovarian cancer. In other words, Sudx\/WWP2 downregulates signalling by polymerised Dishevelled and by Notch, thereby providing cross-talk between these two signalling pathways at the plasma membrane, within the Wnt signalosome.<\/p>\n\n\n