{"id":15,"date":"2018-05-16T17:01:08","date_gmt":"2018-05-16T16:01:08","guid":{"rendered":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/?page_id=15"},"modified":"2020-03-04T12:29:52","modified_gmt":"2020-03-04T12:29:52","slug":"resources","status":"publish","type":"page","link":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/resources\/","title":{"rendered":"Resources"},"content":{"rendered":"<hr \/>\n<h3>Plasmids<\/h3>\n<ul>\n<li>Published plasmids are deposited in Addgene: <a href=\"https:\/\/www.addgene.org\/Andrew_Carter\/\">https:\/\/www.addgene.org\/Andrew_Carter\/<\/a><\/li>\n<li>Do contact us directly for other requests<\/li>\n<\/ul>\n<hr \/>\n<h3>Lab Protocols<\/h3>\n<ul>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/protocols\/2010_Carter_YeastDyneinPrep.pdf\" > ZZ tagged dynein purification from yeast<\/a><\/li>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/protocols\/2020_Carter_Sf9_dynein_prep.docx\" > Human dynein expressed in Sf9 cells<\/a><\/li>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/2020_Carter_dynactin_brain_prep.docx\" > Dynactin purification from pig brain<\/a><\/li>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/protocols\/2020_Carter_TIRF_protocol.docx\" > Dynein\/dynactin TIRF motility assays<\/a><\/li>\n<\/ul>\n<hr \/>\n<h3>Computer Protocols<\/h3>\n<ul>\n<li>Microsoft Word Macros for PCR primer design\n<ul>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/computer\/word_macros\/ReverseComplement.doc\" > Macro to reverse complement highlighted region of DNA sequence. Includes instructions for adding macros to Word<\/a><\/li>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/computer\/word_macros\/TranslateMacro.docx\" > Macro to translate highlighted region of DNA into 1 letter amino acid code<\/a><\/li>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/computer\/word_macros\/TranslateIncNucleotide2.docx\" > Macro to translate highlighted region of DNA into 1 letter amino acid code, includes nucleotide sequence<\/a><\/li>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/resources\/computer\/word_macros\/UsefulMacros.docx\" > Macros to make 20mer forward and reverse primers and 16mer gibson sites<\/a><\/li>\n<\/ul>\n<\/li>\n<li>Making Figures\n<ul>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/download\/Illustrator-for-Scientists-A-Brief-Overview.pdf\" > Tips for using Adobe Illustrator (Ferdos Abid Ali)<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<hr \/>\n<h3>Public Engagement<\/h3>\n<ul>\n<li><a href=\"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera-jmol\/jmol\/ZoomOnDynein.html\">Zooming in on Molecular Motors<\/a>\n<ul>\n<li>An interactive JSmol tutorial Andrew wrote for public engagement events. It introduces you to how proteins are made of amino acids, how they are displayed and how the molecular motor dynein uses ATP to drive movement.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Plasmids Published plasmids are deposited in Addgene: https:\/\/www.addgene.org\/Andrew_Carter\/ Do contact us directly for other requests Lab Protocols Computer Protocols Microsoft Word Macros for PCR primer design Making Figures &nbsp; Public Engagement Zooming in on Molecular Motors An interactive JSmol tutorial Andrew wrote for public engagement events. It introduces you to how proteins are made of [&hellip;]<\/p>\n","protected":false},"author":11,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":"","_links_to":"","_links_to_target":""},"class_list":{"0":"post-15","1":"page","2":"type-page","3":"status-publish","5":"entry"},"featured_image_src":null,"featured_image_src_square":null,"_links":{"self":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/pages\/15","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/comments?post=15"}],"version-history":[{"count":31,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/pages\/15\/revisions"}],"predecessor-version":[{"id":746,"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/pages\/15\/revisions\/746"}],"wp:attachment":[{"href":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/cartera\/wp-json\/wp\/v2\/media?parent=15"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}