This material is supplemental to Ford et al., "Curvature of clathrin coated pits driven by epsin" Nature, 419: 361-366 (.pdf).
Supplementary Table 2 Calorimetric parameters.
|
|
KD (mM) |
KA (M-1) |
DH (kcal mol-1) |
TDS (kcal mol-1) |
|
Epsin1
ENTH WT |
|
|
|
|
|
Ins(1,4)P3 |
No binding |
|
|
|
|
Ins(1,5)P3 |
No binding |
|
|
|
|
Ins(1,3,5)P3 |
�� 120 |
8.6 x
103 |
-11.5 |
-6.4 |
|
Ins(1,4,5)P3
|
�� 3.6 |
2.9 x
105 |
-20.0 |
-12.9 |
|
Ins(1,4,5)P3������������������ 20 �C |
�� 11 |
9.7
x 104 |
-21.1 |
-14.4 |
|
Ins(1,4,5)P3������������������ 25 �C |
�� 29 |
3.4
x 104 |
-21.7 |
-15.5 |
|
Ins(1,4,5)P3������������������ 37 �C |
�� 32 |
3.1 x
104 |
-22.1 |
-15.9 |
|
Ins(1,3,4,5)P3 |
�� 4.1 |
2.4 x
105 |
-17.7 |
-10.7 |
|
InsP6 |
�� 0.55 |
1.8
x 106 |
-16.0 |
-7.9 |
|
diC8PtdIns(4,5)P2 |
�� 0.84 |
1.2 x 106 |
-11.1 |
-3.2 |
|
|
|
|
|
|
|
Epsin1
ENTH L6Q |
|
|
|
|
|
Ins(1,4,5)P3 |
�� 3.0 |
3.3 x 105 |
-20.4 |
-13.2 |
|
diC8PtdIns(4,5)P2 |
�� 2.2 |
4.6 x 105 |
-17.0 |
-9.6 |
|
|
|
|
|
|
|
Epsin1
ENTH L6E |
|
|
|
|
|
Ins(1,4,5)P3 |
�� 5.3 |
1.9 x 105 |
-21.4 |
-14.5 |
|
diC8PtdIns(4,5)P2 |
�� 5.2 |
1.9 x 105 |
-15.0 |
-8.2 |
|
|
|
|
|
|
|
Epsin1
ENTH L6E + I13D |
|
|
|
|
|
Ins(1,4,5)P3 |
�� 27 |
3.7 x 104 |
-19.2 |
-13.3 |
|
|
|
|
|
|
|
Epsin1
ENTH R63L + H73L |
|
|
|
|
|
Ins(1,4,5)P3 |
No binding |
|
|
|
|
|
|
|
|
|
|
AP180
ANTH WT |
|
|
|
|
|
Ins(1,4,5)P3 |
30 |
3.4 x 104 |
-4.8 |
1.1 |
|
InsP6����������� (2 ANTH/InsP6) |
5.1 |
2.2 x 105 |
-4.9 |
2.0 |