This material is supplemental to Ford et al., "Curvature of clathrin coated pits driven by epsin" Nature, 419: 361-366 (.pdf).
Supplementary Table 2
Calorimetric parameters. KD (mM) KA (M-1) DH (kcal mol-1) TDS (kcal mol-1) Epsin1
ENTH WT Ins(1,4)P3 No binding Ins(1,5)P3 No binding Ins(1,3,5)P3 120 8.6 x
103 -11.5 -6.4 Ins(1,4,5)P3
3.6 2.9 x
105 -20.0 -12.9 Ins(1,4,5)P3 20 °C 11 9.7
x 104 -21.1 -14.4 Ins(1,4,5)P3 25 °C 29 3.4
x 104 -21.7 -15.5 Ins(1,4,5)P3 37 °C 32 3.1 x
104 -22.1 -15.9 Ins(1,3,4,5)P3 4.1 2.4 x
105 -17.7 -10.7 InsP6 0.55 1.8
x 106 -16.0 -7.9 diC8PtdIns(4,5)P2 0.84 1.2 x 106 -11.1 -3.2 Epsin1
ENTH L6Q Ins(1,4,5)P3 3.0 3.3 x 105 -20.4 -13.2 diC8PtdIns(4,5)P2 2.2 4.6 x 105 -17.0 -9.6 Epsin1
ENTH L6E Ins(1,4,5)P3 5.3 1.9 x 105 -21.4 -14.5 diC8PtdIns(4,5)P2 5.2 1.9 x 105 -15.0 -8.2 Epsin1
ENTH L6E + I13D Ins(1,4,5)P3 27 3.7 x 104 -19.2 -13.3 Epsin1
ENTH R63L + H73L Ins(1,4,5)P3 No binding AP180
ANTH WT Ins(1,4,5)P3 30 3.4 x 104 -4.8 1.1 InsP6 (2 ANTH/InsP6) 5.1 2.2 x 105 -4.9 2.0