Epsin1 tubulation and membrane bending

Our first indication that epsin affected membrane curvature was the tubulation of PtdIns(4,5)P₂ liposomes. Full-length epsin1 tubulates but only the ENTH domain is necessary. In the presence of clathrin, curvature is localised to regions of clathrin-polymerisation.

more monolayer images....
Thus we believe that epsin drives membrane curvature in a clathrin-coated pit.

Ford, M.G.J., Mills, I.G., Peter, B.J., Vallis, Y., Praefcke, G.J.K., Evans, P.R. and McMahon, H.T. (2002) Curvature of clathrin-coated pits driven by epsin. Nature 419: 361-366 (abstract). (.pdf) supplemental data

(see also News and Views and highlights in Nature Cell Biology, Nature reviews in molecular cell biology, and a mini review in Cell (Cell October 18, 2002: 111 (2):143-146)(summary)

Overview

AP2 Adaptors

Adaptor Home Page

Appendage domains

CCV Assembly

Amphiphysin

Amphiphysin Home Page

BAR Domain

AP180

AP180 Home Page

Clathrin Polymer- isation

ANTH Structure

ANTH versus ENTH Domains

Immuno- fluorescence

BAR Superfamily

BAR /N-BAR

F-BAR

I-BAR

Dynamin

Dynamin Home Page

Dynamin Superfamilies

GTPase Domain

Middle Domain

PH Domain

GED Domain

PRD Domain

EHD (large G-protein)

Endophilin

Epsin1

Epsin1 Home Page

ENTH Structure

Clatharin Assembly

Epsin1 Over- expression

EpsinR

EpsinR Home Page

Immuno- fluorescence

Synaptotagmin

Interactomes

In vitro CCV budding

Tools to inhibit clathrin- dependent endocytosis

Structure gallery

Prediction programme for repeat motifs

Recent Publications

Highlights from the Past

Supple- mental data for Past Publications

Epsin1 paper (Nature)

Dynamin mutant paper (Nature)

EpsinR paper (J.Cell Biol)

BAR Domain (Science)

General Techniques

Isothermal Titration Calorimetry

Liposome Preparation

Inositol Lipid protonation

Lipid Monolayer Assay

Spin Assay

CCV Preparation

GTPase Assay

COS Cell Transfections