Spin assay
This assay is used to test lipid binding, lipid specificity, and recruitment of proteins to liposomes
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Techniques
Overview
AP2 Adaptors
Adaptor Home Page
Appendage domains
CCV Assembly
Amphiphysin
Amphiphysin Home Page
BAR Domain
AP180
AP180 Home Page
Clathrin Polymer- isation
ANTH Structure
ANTH versus ENTH Domains
Immuno- fluorescence
BAR Superfamily
BAR /N-BAR
F-BAR
I-BAR
Dynamin
Dynamin Home Page
Dynamin Superfamilies
GTPase Domain
Middle Domain
PH Domain
GED Domain
PRD Domain
EHD (large G-protein)
Endophilin
Epsin1
Epsin1 Home Page
ENTH Structure
Clatharin Assembly
Epsin1 Over- expression
EpsinR
EpsinR Home Page
Immuno- fluorescence
Synaptotagmin
Interactomes
In vitro CCV budding
Tools to inhibit clathrin- dependent endocytosis
Structure gallery
Prediction programme for repeat motifs
Recent Publications
Highlights from the Past
Supple- mental data for Past Publications
Epsin1 paper (Nature)
Dynamin mutant paper (Nature)
EpsinR paper (J.Cell Biol)
BAR Domain (Science)
General Techniques
Isothermal Titration Calorimetry
Liposome Preparation
Inositol Lipid protonation
Lipid Monolayer Assay
Spin Assay
CCV Preparation
GTPase Assay
COS Cell Transfections
