{"id":294,"date":"2019-12-12T12:57:59","date_gmt":"2019-12-12T12:57:59","guid":{"rendered":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/nagai\/?page_id=294"},"modified":"2020-06-30T14:35:55","modified_gmt":"2020-06-30T13:35:55","slug":"movies-of-splicing","status":"publish","type":"page","link":"https:\/\/www2.mrc-lmb.cam.ac.uk\/groups\/nagai\/movies-of-splicing\/","title":{"rendered":"Movies of splicing"},"content":{"rendered":"

For our recent paper in Annual Review of Biochemistry<\/a>, we made some movies to help explain splicing. The first movie gives a quick overview of the amazing conformational changes in the spliceosome. The next six movies then break down each conformational change and explain what is going on. For movies 2-7, turn the volume up to hear the dulcet narration of our very own Chris Norman! We really hope these movies make splicing understandable, and that they could be useful for teaching etc.<\/p>\n

There are two main points to take away from these movies:
\n1) The spliceosome doesn’t start with an active site! It has to build the active site on the intron out of the U6 snRNA – always shown in red<\/em>.
\n2) Every conformational change gets closer to achieving two goals: making the active site, and bringing the reacting groups of the pre-mRNA into this active site<\/p>\n

If you ever get lost, just remember: the intron is in black, and the two exons are in gold.<\/em><\/p>\n

Movie 1: An overview of pre-mRNA splicing<\/h2>\n

This movie gives a quick view of all the conformational changes that occur when going from an mRNA precursor to a fully spliced mRNA.<\/p>\n