Mechanisms of Macromolecular Machines
Mechanisms of Macromolecular Machines
Characterisation of multi-protein complexes
A main goal of the lab is to understand the molecular mechanisms of addition and removal of mRNA poly(A) tails, performed by Cleavage and Polyadenylation Factor (CPF), Pan2–Pan3 and Ccr4-Not. We also study the Fanconi Anaemia core complex, a large E3 ubiquitin ligase involved in DNA repair, to understand how it monoubiquitinates its substrate proteins (FANCD2-FANCI).
We purify these complexes, reconstitute their biochemical activities and determine their structures to gain mechanistic insights. We use a hybrid approach to establish fundamental principles underlying assembly of multi-protein complexes, define their structures, gain insight into their activities and regulation, and identify roles for proteins of unknown function.
Lori A. Passmore, MRC Laboratory of Molecular Biology, Cambridge
Passmore Lab, MRC Laboratory of Molecular Biology, Cambridge UK