Process movies: Difference between revisions
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Note that all micrographs are in MRC format, and all movies are MRC stacks. (You can also give movie a mrcs extension, as | Note that all micrographs are in MRC format, and all movies are MRC stacks. (You can also give movie a mrcs extension, as in RELION's [[Conventions_%26_File_formats#Image_I.2FO convention]]) need to have the same rootname (e.g. 001, or 24.01.13.15.04, or whatever you like), and that movies have to have the same rootname, followed by a movie-identifier string (in this case "movie") that follows the rootname after an additional "_" character. | ||
First, use the average micrographs (i.e. single-images that are the average of all frames of your movies) to extract "normal" particle stacks, and follow the [[Recommended_procedures]] for 3D refinement. | |||
= Preprocessing movies = | = Preprocessing movies = | ||
Revision as of 16:08, 24 January 2013
As of version 1.2, RELION incorporates functionality to process movies from fast digital cameras, such as direct-electron detection devices (DDDs). The following has been tested on several data sets from our FEI Falcon camera. Other detectors have not been tested yet. Please share your experiences with us if you have.
What to first
Organize all your movies and the average micrographs (i.e. single-images that are the average of all frames of your movies) in the following way, inside one or more directories that are inside your RELION project directory (from where you would launch the GUI).
001.mrc
001_movie.mrc
24.01.13.15.04.mrc
24.01.13.15.04_movie.mrc
myverybest.mrc
myverybest_movie.mrc
...
Note that all micrographs are in MRC format, and all movies are MRC stacks. (You can also give movie a mrcs extension, as in RELION's Conventions_&_File_formats#Image_I.2FO convention) need to have the same rootname (e.g. 001, or 24.01.13.15.04, or whatever you like), and that movies have to have the same rootname, followed by a movie-identifier string (in this case "movie") that follows the rootname after an additional "_" character.
First, use the average micrographs (i.e. single-images that are the average of all frames of your movies) to extract "normal" particle stacks, and follow the Recommended_procedures for 3D refinement.
Preprocessing movies
Then, extract (box out) the individual movie-frames of all particles from the