Analyse results

Output files

For every iteration, RELION will output the following files:

1. rootname_it000???_class000???.mrc (or rootname_it000???_class000???.mrcs for 2D refinements)
2. rootname_it000???_optimiser.star
3. rootname_it000???_mlmodel.star
4. rootname_it000???_data.star

These files contain the following information, respectively:

1. The images of the refined 2D/3D structures.
2. General information about the optimisation process.
3. Information about the refined model parameters apart from the images (e.g. the noise spectra, the spherical average of the signal-to-noise ratios in the reconstructed structures, the distribution of the images over the classes, the angular distributions, etc.
4. For each of the individual experimental images: information about their CTF, optimal orientation, translation and class assignment, normalisation correction, height of the (normalised) probability distribution, etc .

What to look for

For a complete list of all metadata labels, on the command-line type relion_refine --print_metadata_labels.