Helical processing: Difference between revisions

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= Import coordinates =
= Import coordinates =
= Manual picking =
= Template-based semi-autopicking =
= 2D classification =
= Notes on 3D references =
= 3D refinement =
= Mask creation =
= Particle polishing =





Revision as of 16:50, 6 June 2016

Our results

A paper about helical processing in Relion 2.0 is still in preparation.


Definition of helical symmetry in Relion

Usage of relion_helix_toolbox

Import coordinates

Manual picking

Template-based semi-autopicking

2D classification

Notes on 3D references

3D refinement

Mask creation

Particle polishing

References

[1] Kudryashev, Mikhail, et al. "Structure of the type VI secretion system contractile sheath." Cell 160.5 (2015): 952-962. PubMed

[2] Fromm, Simon A., et al. "Seeing tobacco mosaic virus through direct electron detectors." Journal of structural biology 189.2 (2015): 87-97. PubMed

[3] Xu, Hui, et al. "Correction: Structural basis for the prion-like MAVS filaments in antiviral innate immunity." Elife 4 (2015): e07546. PubMed