Local symmetry: Difference between revisions

From Relion
Jump to navigation Jump to search
(Created page with "TODO Shaoda")
 
No edit summary
Line 1: Line 1:
TODO Shaoda
TODO Shaoda
Local symmetry operators for a 3-start 13-microtubule with seam:
The only mask and the density map:
Note: Assume that as seen in the top-view, the proto-filaments are numbered as #1~#13 clockwise. The proto-filaments closest to the seam are #1 and #13 while the one opposite to the seam is numbered as #7. The only mask covers the tubulins along the proto-filament #7. The helical symmetry is defined along each proto-filament: twist = +0.1 degrees, rise = 83.1 Angstroms (every pair of ab-tubulins is treated as the subunit, or the building block of each proto-filament). In the local symmetry STAR file, the rot angles are N*360/13 = N*27.69 degrees and the z translations are N*83.1*1.5/13 = N*9.59 Angstroms (N=-6~+6, without 0). The 12 operators (entries, each consisting of a rot angle and a z translation) show how to move the whole proto-filament #7 onto any of the other 12 by a rotation around the helical axis and a translation along the axis.

Revision as of 01:00, 20 October 2017

TODO Shaoda

Local symmetry operators for a 3-start 13-microtubule with seam: The only mask and the density map: Note: Assume that as seen in the top-view, the proto-filaments are numbered as #1~#13 clockwise. The proto-filaments closest to the seam are #1 and #13 while the one opposite to the seam is numbered as #7. The only mask covers the tubulins along the proto-filament #7. The helical symmetry is defined along each proto-filament: twist = +0.1 degrees, rise = 83.1 Angstroms (every pair of ab-tubulins is treated as the subunit, or the building block of each proto-filament). In the local symmetry STAR file, the rot angles are N*360/13 = N*27.69 degrees and the z translations are N*83.1*1.5/13 = N*9.59 Angstroms (N=-6~+6, without 0). The 12 operators (entries, each consisting of a rot angle and a z translation) show how to move the whole proto-filament #7 onto any of the other 12 by a rotation around the helical axis and a translation along the axis.