Encoded cellular synthesis of non-natural polymersGroup Leader Page
The aim of this project is to develop foundational technologies to convert the cellular protein synthesis machinery into a factory for the synthesis of non-canonical biopolymers (1). The project may involve creating synthetic genomes (2,3), generating synthetic ribosomes (4), and evolving key cellular systems for biopolymer synthesis. There may also be opportunities to develop approaches for developing methods for gaining unprecedented insight into biological processes through encoding non natural amino acids into proteins (5)
- Chin, J.W. (2017)
Expanding and Reprogamming the Genetic Code.
Nature. 550: 53-60.
- K. Wang, D. de la Torre, W. E. Robertson, J.W. Chin. (2019)
Programmed chromosome fission and fusion enable precise large-scale genome rearrangements and assembly.
Science 365: 922-926.
- J. Fredens, K. Wang, D. de la Torre, L. F. H. Funke, W. E. Robertson, Y. Christova, T. Chia, W. H. Schmied, D. L. Dunkelmann, V. Beranek, C. Uttamapinant, A. Gonzalez Llamazares, T. S. Elliott, J. W. Chin. (2019)
Total synthesis of Escherichia coli with a recoded genome.
Nature 569: 514-518.
- W. H. Schmied, Z. Tnimov, C. Uttamapinant, C. D. Rae, S. D. Fried, J.W. Chin. (2018)
Controlling orthogonal ribosome subunit interactions enables evolution of new function.
Nature 564: 444-448.
- N. Huguenin-Dezot, D. A. Alonzo, G. W. Heberlig, M. Mahesh, D. P. Nguyen, M. H. Dornan, C. N. Boddy, T. M. Schmeing, J. W. Chin. (2019)
Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid.
Nature 565: 112-117.