This list denotes the number of functional VH, VK, VL, D, JH, JK and JL segments cut by a range of commonly used restriction enzymes. When designing primers and vectors for cloning V genes you may wish to avoid using cloning sites that are found in human V genes. If you want any other restriction enzymes to be included please send me an e-mail.

                                            Number of functional segments which are cut
Restriction    Sequence                        (total number of functional segments)
enzyme         cleaved         VH (51)  VK (40)  VL (31)  D (25)   JH (6)   JK (5)   JK (4)   TOTAL (162)
ApaLI          GTGCAC          2        0        2        0        0        0        0        4
AscI           GGCGCGCC        0        0        0        0        0        0        0        0
BamHI          GGATCC          14       0        15       0        0        0        0        29
BglII          AGATCT          12       0        0        0        0        0        0        12
BssHII         GCGCGC          0        0        0        0        0        0        0        0
BstEII         GGTNACC         4        0        8        0        5        0        1        18
ClaI           ATCGAT          0        0        0        0        0        0        0        0
EagI           CGGCCG          23       0        0        0        0        0        0        23
EcoRI          GAATTC          0        4        0        0        0        0        0        4
EcoRV          GATATC          1        0        0        0        1        1        0        3
HaeIII         (AG)GCGC(CT)    3        4        3        0        0        0        0        10
HindIII        AAGCTT          1        0        2        0        0        0        0        3
KpnI           GGTACC          0        18       23       0        0        0        0        41
NcoI           CCATGG          0        0        1        0        0        0        0        1
NdeI           CATATG          4        0        3        0        0        0        0        7
NheI           GCTAGC          0        0        0        0        0        0        0        0
NotI           GCGGCCGC        0        0        0        0        0        0        0        0
PstI           CTGCAG          14       33       4        0        0        0        0        51
PvuI           CGATCG          0        0        1        0        0        0        0        1
SacI           GAGCTC          11       1        1        0        0        0        0        13
SacII          CCGCGG          8        0        0        0        0        0        0        8
SalI           GTCGAC          0        0        0        0        0        0        0        0
SmaI           CCCGGG          2        1        4        0        0        0        0        7
SpeI           ACTAGT          2        0        1        0        0        0        0        3
SphI           GCATGC          3        9        1        0        0        0        0        13
SfiI           GGCCNNNNNGGCC   0        0        0        0        0        0        0        0
XbaI           TCTAGA          0        0        0        0        0        0        0        0
XhoI           CTCGAG          0        3        0        0        0        0        0        3