This list denotes the number of functional VH, VK, VL, D, JH, JK and JL segments cut by a range of commonly used restriction enzymes. When designing primers and vectors for cloning V genes you may wish to avoid using cloning sites that are found in human V genes.
If you want any other restriction enzymes to be included please send me an e-mail.
Number of functional segments which are cut
Restriction Sequence (total number of functional segments)
enzyme cleaved VH (51) VK (40) VL (31) D (25) JH (6) JK (5) JK (4) TOTAL (162)
ApaLI GTGCAC 2 0 2 0 0 0 0 4
AscI GGCGCGCC 0 0 0 0 0 0 0 0
BamHI GGATCC 14 0 15 0 0 0 0 29
BglII AGATCT 12 0 0 0 0 0 0 12
BssHII GCGCGC 0 0 0 0 0 0 0 0
BstEII GGTNACC 4 0 8 0 5 0 1 18
ClaI ATCGAT 0 0 0 0 0 0 0 0
EagI CGGCCG 23 0 0 0 0 0 0 23
EcoRI GAATTC 0 4 0 0 0 0 0 4
EcoRV GATATC 1 0 0 0 1 1 0 3
HaeIII (AG)GCGC(CT) 3 4 3 0 0 0 0 10
HindIII AAGCTT 1 0 2 0 0 0 0 3
KpnI GGTACC 0 18 23 0 0 0 0 41
NcoI CCATGG 0 0 1 0 0 0 0 1
NdeI CATATG 4 0 3 0 0 0 0 7
NheI GCTAGC 0 0 0 0 0 0 0 0
NotI GCGGCCGC 0 0 0 0 0 0 0 0
PstI CTGCAG 14 33 4 0 0 0 0 51
PvuI CGATCG 0 0 1 0 0 0 0 1
SacI GAGCTC 11 1 1 0 0 0 0 13
SacII CCGCGG 8 0 0 0 0 0 0 8
SalI GTCGAC 0 0 0 0 0 0 0 0
SmaI CCCGGG 2 1 4 0 0 0 0 7
SpeI ACTAGT 2 0 1 0 0 0 0 3
SphI GCATGC 3 9 1 0 0 0 0 13
SfiI GGCCNNNNNGGCC 0 0 0 0 0 0 0 0
XbaI TCTAGA 0 0 0 0 0 0 0 0
XhoI CTCGAG 0 3 0 0 0 0 0 3