PreProcessing: Difference between revisions
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(Created page with "As of version 1.1, the RELION GUI implements a semi-automated data preprocessing procedure. This procedures consists of three steps, each of which may be performed independent...") |
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As of version 1.1, the RELION GUI implements a semi-automated data preprocessing procedure. This procedures consists of three steps, each of which may be performed independently. The advantage of this procedure is ease-of-use (suitable STAR files and images are created with minimal user intervention) as well as speed (all three steps are MPI-parallelized). | As of version 1.1, the RELION GUI implements a semi-automated data preprocessing procedure. This procedures consists of three steps, each of which may be performed independently. The advantage of this procedure is ease-of-use (suitable STAR files and images are created with minimal user intervention) as well as speed (all three steps are MPI-parallelized). | ||
== CTF estimation == | |||
The relion_preprocess program will launch the CTFFIND3 executable as indicated on the GUI. Apart from the general microscopy settings on the first tab of the GUI, the second tab contains entries for all usual CTFFIND3 parameters. Use wildcards (filenames with * or ?) to select multiple micrographs (in MRC format!) to work on. | |||
=== Particle extraction === | === Particle extraction === | ||
=== Particle operations === | === Particle operations === | ||
Revision as of 14:46, 11 September 2012
As of version 1.1, the RELION GUI implements a semi-automated data preprocessing procedure. This procedures consists of three steps, each of which may be performed independently. The advantage of this procedure is ease-of-use (suitable STAR files and images are created with minimal user intervention) as well as speed (all three steps are MPI-parallelized).
CTF estimation
The relion_preprocess program will launch the CTFFIND3 executable as indicated on the GUI. Apart from the general microscopy settings on the first tab of the GUI, the second tab contains entries for all usual CTFFIND3 parameters. Use wildcards (filenames with * or ?) to select multiple micrographs (in MRC format!) to work on.