Preprocess images: Difference between revisions
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(Created page with "RELION does not discard any image during refinement. Therefore, data sets should be clean from ''false'' particles.") |
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RELION does not discard any image during refinement. Therefore, data sets should be clean from ''false'' particles. | RELION does not discard any image during refinement. Therefore, data sets should be clean from ''false'' particles. Masking and CTF correction are done internally, so in the preprocessing images do not need to be masked, nor CTF phase-flipped or Wiener-filtered. The images should be normalised, but the exact procedures do not matter much, as also normalisation errors may be corrected inside the refinement. | ||
CTF phase-flipping | |||
Revision as of 20:36, 27 September 2011
RELION does not discard any image during refinement. Therefore, data sets should be clean from false particles. Masking and CTF correction are done internally, so in the preprocessing images do not need to be masked, nor CTF phase-flipped or Wiener-filtered. The images should be normalised, but the exact procedures do not matter much, as also normalisation errors may be corrected inside the refinement.
CTF phase-flipping