Some of the main consumables can be obtained from Stores: 96-well MRC crystallisation plates, screening kits in tube format and additive screens in plate format.
Other main consumables, such as CrystalClear tape, are provided in the facility. Tips for the robots are ordered separately by the responsible of the Facility. In the eventuality of purchasing consumables for communal purposes, please use the cost centre specific to the crystallisation facility: MC-A021-53017. Please do not abuse this cost centre (!).
Note that crystallisation kits should not be withdrawn from Stores for your own use, even if using your own cost centre number. They are solely reserved for routine preparation of LMB plates. If you plan intensive use of a particular screen, please order it directly from the supplier (links below) with your group's cost centre.
Also not part of the crystallisation facility cost centre are plates/reagents/tubes to prepare optimization screens. Basically, consumables related to optimization experiments must be purchased with your group's cost centre. Although we may have some (free) 48-well MRC MAXI plates available within the facility.
| Name of Reagent/ Equipment | Company | Catalog Number | Comments/Description |
|---|---|---|---|
| 96-well crystallization plate | Swissci | MRC 96T-UVP | The original MRC 2-drop plate. Reservoir recommended volume: 80 µL. Range of useful droplet volumes: 10-1000 nL. UV transmissible. |
| Initial screening kits in tubes | Multiple | n/a | See page 'LMB plates'. Only designated people can withdraw kits from Stores. |
| MORPHEUS additive screen | Molecular Dimensions | n/a | Frozen in 96-well MRC plate (8 µL per well). |
| ANGSTROM additive screen | Molecular Dimensions | n/a | Frozen in 96-well MRC plate (8 µL per well). |
| Name of Reagent/ Equipment | Company | Catalog Number | Comments/Description |
|---|---|---|---|
| 48-well crystallization plate | Swissci | MMX01-UVP | The MRC MAXI 1-drop plate for scale-up/optimization. Reservoir recommended volume: 200 µL. Range of useful droplet volumes: 0.1-10 µL. UV transmissible. |
| Adhesive clear tape | Hampton Research | HR4-50 | 3-inch wide 'Duck HD-Clear' tape for sealer and manual sealing. |
| Mosquito tips | TTP Labtech | 4150-03020 | Setting up crystallization droplets (Mosquito). Spool of 26,000 tips (9mm spacing). |
| 2 µL 8-well strip | TTP Labtech | 4150-03110 | Contains protein for crystallization droplets (Mosquito). Box of 40 strips, max. vol. in well is 3.2 µL. |
| 5 µL 8-well strip | TTP Labtech | 4150-03100 | Contains protein for crystallization droplets (Mosquito). Box of 40 strips, max. vol. in well is 7.5 µL. |
| 5 mL syringes | TTP Labtech | 4150-07100 | Preparing optimization screen (Dragonfly). Pack of 100. |
| Troughs | TTP Labtech | 4150-07103 | Preparing optimization screen (Dragonfly). Pack of 50. |
| Ink cartridge | Markem-Imaje | 9651 | System 1 (inkjet printer). |
| Solvent cartridge | Markem-Imaje | 8652 | System 1 (inkjet printer). |
| CO-RE Tips (50 µL) | Hamilton | 235947 | System 2 (liquid handler). Box of 6 sets with 1920 tips in disposable stacks. |
| PCR plate | ThermoScientific | AB-2150 | System 2 (liquid handler). Abgene Diamond ultra, 384 V-shaped wells. |
| Name of Reagent/ Equipment | Company | Catalog Number | Comments/Description |
|---|---|---|---|
| MORPHEUS additive screen | Molecular Dimensions | MD1-93 | Frozen in 96-well cell culture 'Costar' plate with V-shaped wells (100 µL per well). |
| ANGSTROM additive screen | Molecular Dimensions | MD1-100-FX | Frozen in 96-well cell culture 'Costar' plate with V-shaped well (100 µL per well). |
| Adhesive aluminium sheet | Beckman Coulter | 538619 | Used to reseal additive screens. |
| HT additive screen | Hampton Research | HR2-138 | Frozen in 96-well deepwell block (1 mL per well). |
Fabrice Gorrec, room 2N155, (+44 1223 26) 7807, fgorrec@mrc-lmb.cam.ac.uk
alternatively:
Jan Löwe, room 2N159, (+44 1223 26) 7064, jyl@mrc-lmb.cam.ac.uk
Olga Perisic, lab 2S069, (+44 1223 26) 7893, op1@mrc-lmb.cam.ac.uk
Last Updated 2/12/15