MRC-LMB Crystallisation Facility: LMB plates

Left hand side: MS Excel score sheet for that plate.

Manufacturer's original documentation can be found on the LMB Screen Database page.

Please find links to manufacturer's websites on the Consumables page.

Plate name kit supplier CN* tubes basic description
LMB01 Crystal Screen 1 Hampton Research HR2-110 48 Sparse Matrix (pH 4.6-8.5)
Crystal Screen 2 Hampton Research HR2-112 48 Stochastic sampling (pH 4.6-9.0)
LMB02 Wizard 1 Rigaku 1009530 48 Stochastic sampling (pH 4.5-10.5)
Wizard 2 Rigaku 1009531 48 Stochastic sampling (pH 4.5-10.5)
LMB03 Grid Screen Ammonium Sulfate Hampton Research HR2-211 24 Grid screen, conc. AmS = 0.8-3.2 M and buffers pH 4.0-9.0
Grid Screen PEG/LiCl Hampton Research HR2-217 24 Grid screen, conc. PEG 6000 = 0-30 %w/v, conc. LiCl = 1.0 M and buffers pH 4.0-9.0
Quick Screen Hampton Research HR2-221 24 Grid screen, conc. NaKPO4 = 0.8-1.8 M at pH 5.0-8.2
Grid Screen Sodium Chloride Hampton Research HR2-219 24 Grid screen, conc. NaCl = 1.0-4.0 M and buffers pH 4.0-9.0
LMB04 Grid Screen PEG 6000 Hampton Research HR2-213 24 Grid screen, conc. PEG 6000 = 5-30 %w/v and buffers pH 4.0-9.0
Grid Screen MPD Hampton Research HR2-215 24 Grid screen conc. MPD = 10-65 %w/v and buffers pH 4.0-9.0
MemFac Hampton Research HR2-114 48 Sparse Matrix for membrane proteins (pH 4.6-8.5)
LMB05 PEG-Ion Hampton Research HR2-126 48 Grid screen, conc. PEG 3350 = 20% w/v and various salts at 0.2 M (no buffers)
Natrix Hampton Research HR2-116 48 Incomplete factorial (pH 5.6-8.5)
LMB06b Crystal Screen Lite Hampton Research HR2-128 48 Crystal Screen 1 with one-half of the original precipitant concentrations
Custom Lite screen Molecular Dimensions LMB-CUSTOMLITE 48 More conditons with low precipitant concentrations
LMB07 Wizard Cryo 1 Rigaku 1009536 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-9.4)
Wizard Cryo 2 Rigaku 1009537 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-10.1)
LMB08 JBS1 JenaBioScience CS-101L 24 Incomplete factorial based on various PEGs (pH 4.6-9.0)
JBS2 JenaBioScience CS-102L 24 Incomplete factorial based on PEG 4000 (pH 4.6-8.5)
JBS3 JenaBioScience CS-103L 24 Incomplete factorial based on PEG 4000 (pH 4.6-8.5)
JBS4 JenaBioScience CS-104L 24 Incomplete factorial based on medium MW PEGs (pH 6.5-8.5)
LMB09 JBS5 JenaBioScience CS-105L 24 Incomplete factorial based on heavy MW PEGs (pH 6.5-9.5)
JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5)
JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5)
JBS8 JenaBioScience CS-108L 24 Incomplete factorial based on MPD and ethanol (pH 4.6-8.5)
LMB10 JBS9 JenaBioScience CS-109L 24 Incomplete factorial based on common salts and 2-propanol (pH 4.6-8.5)
JBS10 JenaBioScience CS-110L 24 Incomplete factorial based on common salts (pH 4.6-8.5)
Clear Strategy Screen 1 pH 4.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 5.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
LMB11 Clear Strategy Screen 1 pH 6.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 7.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 8.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 2 pH 4.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
LMB12 Clear Strategy Screen 2 pH 5.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
Clear Strategy Screen 2 pH 6.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
Clear Strategy Screen 2 pH 7.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
Clear Strategy Screen 2 pH 8.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
LMB13 Index Hampton Research HR2-144 96 Mix of Sparse Matrix and Grid screens (pH 3.0-9.0)
LMB14 SaltRX 1 Hampton Research HR2-107 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
SaltRX 2 Hampton Research HR2-109 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
LMB15 MemStart Molecular Dimensions MD1-21 48 Sparse Matrix for membrane proteins (pH 4.0-10.0)
MemSys Molecular Dimensions MD1-25 48 Grid screen for membrane proteins (mostly PEGs, pH 3.5-9.5)
LMB16 JCSG+ Qiagen n/a 96 Sparse Matrix (pH 4.0-10.0)
LMB17 Morpheus screen Molecular Dimensions MD1-46 96 Grid screen including mixes of additives and cryoprotected conditions (pH 6.5-8.5)
LMB18 Pi minimal screen JenaBioScience CS-127 96 Incomplete Factorial (pH 4.0-9.5)
LMB19 Pi-PEG screen JenaBioScience CS-128 96 Incomplete factorial for membrane proteins (pH 4.8-8.8)
LMB20 Morpheus II screen Molecular Dimensions MD1-91 96 Grid screen including mixes of additives (heavy atoms) and cryoprotected conditions (pH 6.5-8.5)
LMB21 LMB crystallisation screen Molecular Dimensions MD1-98 96 Sparse Matrix including conditions from LMB publications.
LMB22 Morpheus III screen Molecular Dimensions n/a 96 Grid screen including mixes of additives (drug compounds) and cryoprotected conditions (pH 6.5-8.5).
* Catalogue number for 10 ml, tube format.

Other plates available

Plate name kit supplier CN tubes basic description
SOLUB JBScreen Solubility HTS JenaBioScience 96 Pre-crystallisation tests for buffer, pH, salt and glycerol.
Addit01 Morpheus additive screen Molecular Dimensions MD1-93-MRC 96 All the single reagents used to formulate the initial screens Morpheus I and II. Frozen plates.
Addit02 Angstrom additve screen Molecular Dimensions MD1-100-MRC 96 Single cryoprotecting polyols found at three different concentrations. Frozen plates.
Addit03 Hippocrates additive screen Molecular Dimensions n/a 48 All the single additives used to formulate the initial screen Morpheus III. Frozen plates (additives in the first half only).
Addit04 Detergent screen Hampton Research HR2-407 96 96 detergents from different classes (Ionic, Non-ionic, Zwitterionic and NDSB).

Back

Last Updated 2/12/15