MRC-LMB Crystallisation Facility: pre-filled MRC plates

LMB plates are 2-drop MRC crystallisation plates (MRC96T-UVP,), which reservoirs are pre-filled with different screening kits.(final volume in reservoirs: 80 μL). The LMB plates are stored in the 10°C incubator of the protein crystallisation facility, room 1S205.

Details of the LMB plates can be found in the Table below, which can also be downloaded here Analysing results is facilitated with the LMB screen database

There are 18 LMB plates x 96 conditions = 1,728 conditions in total.18 LMB plates x 16 μL = 288 μL is the amount required for a full initial screen with the protocol "1 drop 100 nl" on the Mosquito robot.

LMB plates were prepared with great care to reduce the condensation on the inner seal.Some condensation may however still occur when conditions contain volatile reagents (notably MPD and ethanol).

Please seek advice from the Protein Crystallisation Facility Manager (Fabrice Gorrec, ext. 7807, fgorrec@mrc-lmb.cam.ac.uk)

No. Plate name kit supplier Cat No* conditions basic description
1 LMB01 Crystal Screen 1 Hampton Research HR2-110 48 Sparse Matrix (pH 4.6-8.5)
Crystal Screen 2 Hampton Research HR2-112 48 Stochastic sampling (pH 4.6-9.0)
2 LMB02 Wizard 1 Rigaku 1009530 48 Stochastic sampling (pH 4.5-10.5)
Wizard 2 Rigaku 1009531 48 Stochastic sampling (pH 4.5-10.5)
3 LMB03 Grid Screen Ammonium Sulfate Hampton Research HR2-211 24 Grid screen, conc. AmS = 0.8-3.2 M and buffers pH 4.0-9.0
Grid Screen PEG/LiCl Hampton Research HR2-217 24 Grid screen, conc. PEG 6000 = 0-30 %w/v, conc. LiCl = 1.0 M and buffers pH 4.0-9.0
Quick Screen Hampton Research HR2-221 24 Grid screen, conc. NaKPO4 = 0.8-1.8 M at pH 5.0-8.2
Grid Screen Sodium Chloride Hampton Research HR2-219 24 Grid screen, conc. NaCl = 1.0-4.0 M and buffers pH 4.0-9.0
4 LMB04 Grid Screen PEG 6000 Hampton Research HR2-213 24 Grid screen, conc. PEG 6000 = 5-30 %w/v and buffers pH 4.0-9.0
Grid Screen MPD Hampton Research HR2-215 24 Grid screen conc. MPD = 10-65 %w/v and buffers pH 4.0-9.0
MembFac Hampton Research HR2-114 48 Sparse Matrix for membrane proteins (pH 4.6-8.5)
5 LMB05 PEG-Ion Hampton Research HR2-126 48 Grid screen, conc. PEG 3350 = 20% w/v and various salts at 0.2 M (no buffers)
Natrix Hampton Research HR2-116 48 Incomplete factorial (pH 5.6-8.5)
6 LMB07 Wizard Cryo 1 Rigaku 1009536 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-9.4)
Wizard Cryo 2 Rigaku 1009537 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-10.1)
7 LMB09 JBS5 JenaBioScience CS-105L 24 Incomplete factorial based on heavy MW PEGs (pH 6.5-9.5)
JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5)
JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5)
JBS8 JenaBioScience CS-108L 24 Incomplete factorial based on MPD and ethanol (pH 4.6-8.5)
8 LMB10 JBS9 JenaBioScience CS-109L 24 Incomplete factorial based on common salts and 2-propanol (pH 4.6-8.5)
JBS10 JenaBioScience CS-110L 24 Incomplete factorial based on common salts (pH 4.6-8.5)
Clear Strategy Screen 1 pH 4.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 5.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
9 LMB14 SaltRX 1 Hampton Research HR2-107 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
SaltRX 2 Hampton Research HR2-109 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
10 LMB15 MemStart Molecular Dimensions MD1-21 48 Sparse Matrix for membrane proteins (pH 4.0-10.0)
MemSys Molecular Dimensions MD1-25 48 Grid screen for membrane proteins (mostly PEGs, pH 3.5-9.5)
11 LMB16 JCSG+ Qiagen 130720  96 Sparse Matrix (pH 4.0-10.0)
12 LMB17 Morpheus screen Molecular Dimensions MD1-46 96 Grid screen including mixes of additives and cryoprotected conditions (pH 6.5-8.5)
13 LMB20 Morpheus II screen Molecular Dimensions MD1-91 96 Grid screen including mixes of additives (heavy atoms) and cryoprotected conditions (pH 6.5-8.5)
14 LMB21 LMB crystallisation screen Molecular Dimensions MD1-98 96 Sparse Matrix including conditions from LMB publications.
15 LMB23 Morpheus FUSION Molecular Dimensions MD1-129  96 Incomplete factorial including mixes of additives from the 3 Morpheus screens, cryoprotected conditions, pH 6.5-8.5.
16 LMB24 Helix Molecular Dimensions MD1-689  96 Sparse matrix optimised for DNA, RNA or DNA/RNA hybrids.
17 LMB23 CSIRO organics Molecular Dimensions MD1-150 96 Sparse matrix integrating various organic compounds and polymers as precipitants (pH range 4.2 - 9.5)
18 LMB24 MORPHEUS SZI n/a n/a n/a Morpheus grid screen formulation with acidic pH range (4.5-7.5) and upgraded additive mixes.
* Catalogue number for 10 ml, tube format.

Back

Last Updated 24/02/26