MRC-LMB Crystallisation Facility: pre-filled MRC plates

Left hand side: MS Excel score sheet for that plate.

Manufacturer's original documentation can be found on the LMB Screen Database page.

Please find links to manufacturer's websites on the Consumables page.

LMB plates: initial screening kits (80μl in reservoirs). Stored in Screen Banks 1 and 2 (10°C incubators).

Plate name kit supplier Cat No* tubes basic description
LMB01 Crystal Screen 1 Hampton Research HR2-110 48 Sparse Matrix (pH 4.6-8.5)
Crystal Screen 2 Hampton Research HR2-112 48 Stochastic sampling (pH 4.6-9.0)
LMB02 Wizard 1 Rigaku 1009530 48 Stochastic sampling (pH 4.5-10.5)
Wizard 2 Rigaku 1009531 48 Stochastic sampling (pH 4.5-10.5)
LMB03 Grid Screen Ammonium Sulfate Hampton Research HR2-211 24 Grid screen, conc. AmS = 0.8-3.2 M and buffers pH 4.0-9.0
Grid Screen PEG/LiCl Hampton Research HR2-217 24 Grid screen, conc. PEG 6000 = 0-30 %w/v, conc. LiCl = 1.0 M and buffers pH 4.0-9.0
Quick Screen Hampton Research HR2-221 24 Grid screen, conc. NaKPO4 = 0.8-1.8 M at pH 5.0-8.2
Grid Screen Sodium Chloride Hampton Research HR2-219 24 Grid screen, conc. NaCl = 1.0-4.0 M and buffers pH 4.0-9.0
LMB04 Grid Screen PEG 6000 Hampton Research HR2-213 24 Grid screen, conc. PEG 6000 = 5-30 %w/v and buffers pH 4.0-9.0
Grid Screen MPD Hampton Research HR2-215 24 Grid screen conc. MPD = 10-65 %w/v and buffers pH 4.0-9.0
MembFac Hampton Research HR2-114 48 Sparse Matrix for membrane proteins (pH 4.6-8.5)
LMB05 PEG-Ion Hampton Research HR2-126 48 Grid screen, conc. PEG 3350 = 20% w/v and various salts at 0.2 M (no buffers)
Natrix Hampton Research HR2-116 48 Incomplete factorial (pH 5.6-8.5)
LMB06b Crystal Screen Lite Hampton Research HR2-128 48 Crystal Screen 1 with one-half of the original precipitant concentrations
Custom Lite screen Molecular Dimensions LMB-CUSTOMLITE 48 More conditons with low precipitant concentrations
LMB07 Wizard Cryo 1 Rigaku 1009536 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-9.4)
Wizard Cryo 2 Rigaku 1009537 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-10.1)
LMB08 JBS1 JenaBioScience CS-101L 24 Incomplete factorial based on various PEGs (pH 4.6-9.0)
JBS2 JenaBioScience CS-102L 24 Incomplete factorial based on PEG 4000 (pH 4.6-8.5)
JBS3 JenaBioScience CS-103L 24 Incomplete factorial based on PEG 4000 (pH 4.6-8.5)
JBS4 JenaBioScience CS-104L 24 Incomplete factorial based on medium MW PEGs (pH 6.5-8.5)
LMB09 JBS5 JenaBioScience CS-105L 24 Incomplete factorial based on heavy MW PEGs (pH 6.5-9.5)
JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5)
JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5)
JBS8 JenaBioScience CS-108L 24 Incomplete factorial based on MPD and ethanol (pH 4.6-8.5)
LMB10 JBS9 JenaBioScience CS-109L 24 Incomplete factorial based on common salts and 2-propanol (pH 4.6-8.5)
JBS10 JenaBioScience CS-110L 24 Incomplete factorial based on common salts (pH 4.6-8.5)
Clear Strategy Screen 1 pH 4.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 5.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
LMB11 Clear Strategy Screen 1 pH 6.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 7.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 8.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 2 pH 4.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
LMB12 Clear Strategy Screen 2 pH 5.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
Clear Strategy Screen 2 pH 6.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
Clear Strategy Screen 2 pH 7.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
Clear Strategy Screen 2 pH 8.5 Molecular Dimensions MD1-16LMB 24 Stochastic sampling
LMB13 Index Hampton Research HR2-144 96 Mix of Sparse Matrix and Grid screens (pH 3.0-9.0)
LMB14 SaltRX 1 Hampton Research HR2-107 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
SaltRX 2 Hampton Research HR2-109 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
LMB15 MemStart Molecular Dimensions MD1-21 48 Sparse Matrix for membrane proteins (pH 4.0-10.0)
MemSys Molecular Dimensions MD1-25 48 Grid screen for membrane proteins (mostly PEGs, pH 3.5-9.5)
LMB16 JCSG+ Qiagen 130720  96 Sparse Matrix (pH 4.0-10.0)
LMB17 Morpheus screen Molecular Dimensions MD1-46 96 Grid screen including mixes of additives and cryoprotected conditions (pH 6.5-8.5)
LMB18 Pi minimal screen JenaBioScience CS-127 96 Incomplete Factorial (pH 4.0-9.5)
LMB19b Pi-PEG [x1.5] Molecular Dimensions n/a 96 PEG-based Incomplete factorial (pH 4.8-8.8). High concentrations
LMB20 Morpheus II screen Molecular Dimensions MD1-91 96 Grid screen including mixes of additives (heavy atoms) and cryoprotected conditions (pH 6.5-8.5)
LMB21 LMB crystallisation screen Molecular Dimensions MD1-98 96 Sparse Matrix including conditions from LMB publications.
LMB22 Morpheus III screen Molecular Dimensions MD1-116  96 Grid screen including mixes of additives (drug compounds) and cryoprotected conditions (pH 6.5-8.5).
LMB23 Morpheus FUSION Molecular Dimensions MD1-129  96 Incomplete factorial including mixes of additives from the 3 Morpheus screens, cryoprotected conditions, pH 6.5-8.5.
* Catalogue number for 10 ml, tube format.

Addit plates: additive screening kits (8μl in reservoirs). Stored in Screen Bank 3 (-20°C Freezer).

Plate name kit supplier Cat No tubes basic description
Addit01 Morpheus additive screen Molecular Dimensions MD1-93-MRC 96 All the single reagents used to formulate the initial screens Morpheus I and II. Frozen plates.
Addit02 Angstrom additve screen Molecular Dimensions MD1-100-MRC 96 Single cryoprotecting polyols found at three different concentrations. Frozen plates.
Addit03 Hippocrates additive screen Molecular Dimensions MD1-118-MRC 48 All the single additives used to formulate the initial screen Morpheus III. Frozen plates (additives in the first half only).
Addit04b Detergent screen Molecular Dimensions AL-SEL-DP 96 Detergents from different classes (Analytic Selector Kit, Anatrace). Frozen plates.
Addit05 Buffer screen Molecular Dimensions 132983-8UL 48 Buffer gradient (Nextal) pH 2.2-11.6 Nextal. Frozen plates (additives in the first half only).

Other plates available: solubility screen (80μl in reservoirs, cold room 1S230).

Plate name kit supplier Cat No tubes basic description
SOLUB JBScreen Solubility HTS JenaBioScience CO-311-CSTM 96 Pre-crystallisation tests for buffer, pH, salt and glycerol.

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Last Updated 2/12/15