MRC-LMB Crystallisation Facility: pre-filled MRC plates

Left hand side: MS Excel score sheet for that plate.

Manufacturer's original documentation can be found on the LMB Screen Database page.

Please find links to manufacturer's websites on the Consumables page.

LMB plates: initial screening kits (80μl in reservoirs) stored in the 10°C incubator (named 'Screen Bank').

The LMB plates 06b, 08, 09, 11, 12, 13, 18, 19 and 22 are discontinued since December 2025.

With three new screens added HELIX, CSIRO organic and MORPHEUS Z, the total number of LMB plate is 17 (i.e. 1634 conditions).

Using the 17 LMB plates with the protocol "1 drop 100 nl" on the Mosquito robot will require 17x16µL = 272µL of sample.

The list of LMB plates described here can be downloaded here.

No. Plate name kit supplier Cat No* conditions basic description
1 LMB01 Crystal Screen 1 Hampton Research HR2-110 48 Sparse Matrix (pH 4.6-8.5)
Crystal Screen 2 Hampton Research HR2-112 48 Stochastic sampling (pH 4.6-9.0)
2 LMB02 Wizard 1 Rigaku 1009530 48 Stochastic sampling (pH 4.5-10.5)
Wizard 2 Rigaku 1009531 48 Stochastic sampling (pH 4.5-10.5)
3 LMB03 Grid Screen Ammonium Sulfate Hampton Research HR2-211 24 Grid screen, conc. AmS = 0.8-3.2 M and buffers pH 4.0-9.0
Grid Screen PEG/LiCl Hampton Research HR2-217 24 Grid screen, conc. PEG 6000 = 0-30 %w/v, conc. LiCl = 1.0 M and buffers pH 4.0-9.0
Quick Screen Hampton Research HR2-221 24 Grid screen, conc. NaKPO4 = 0.8-1.8 M at pH 5.0-8.2
Grid Screen Sodium Chloride Hampton Research HR2-219 24 Grid screen, conc. NaCl = 1.0-4.0 M and buffers pH 4.0-9.0
4 LMB04 Grid Screen PEG 6000 Hampton Research HR2-213 24 Grid screen, conc. PEG 6000 = 5-30 %w/v and buffers pH 4.0-9.0
Grid Screen MPD Hampton Research HR2-215 24 Grid screen conc. MPD = 10-65 %w/v and buffers pH 4.0-9.0
MembFac Hampton Research HR2-114 48 Sparse Matrix for membrane proteins (pH 4.6-8.5)
5 LMB05 PEG-Ion Hampton Research HR2-126 48 Grid screen, conc. PEG 3350 = 20% w/v and various salts at 0.2 M (no buffers)
Natrix Hampton Research HR2-116 48 Incomplete factorial (pH 5.6-8.5)
Custom Lite screen Molecular Dimensions LMB-CUSTOMLITE 48 More conditons with low precipitant concentrations
6 LMB07 Wizard Cryo 1 Rigaku 1009536 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-9.4)
Wizard Cryo 2 Rigaku 1009537 48 Stochastic sampling with conditions cryoprotected using low MW PEGs (pH 4.5-10.1)
JBS2 JenaBioScience CS-102L 24 Incomplete factorial based on PEG 4000 (pH 4.6-8.5)
JBS3 JenaBioScience CS-103L 24 Incomplete factorial based on PEG 4000 (pH 4.6-8.5)
JBS4 JenaBioScience CS-104L 24 Incomplete factorial based on medium MW PEGs (pH 6.5-8.5)
7 LMB10 JBS9 JenaBioScience CS-109L 24 Incomplete factorial based on common salts and 2-propanol (pH 4.6-8.5)
JBS10 JenaBioScience CS-110L 24 Incomplete factorial based on common salts (pH 4.6-8.5)
Clear Strategy Screen 1 pH 4.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
Clear Strategy Screen 1 pH 5.5 Molecular Dimensions MD1-16LMB 24 Grid screen with various PEGs
8 LMB14 SaltRX 1 Hampton Research HR2-107 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
SaltRX 2 Hampton Research HR2-109 48 Grid screen including 22 unique salts versus salt concentration and pH (4.1-9.0)
9 LMB15 MemStart Molecular Dimensions MD1-21 48 Sparse Matrix for membrane proteins (pH 4.0-10.0)
MemSys Molecular Dimensions MD1-25 48 Grid screen for membrane proteins (mostly PEGs, pH 3.5-9.5)
10 LMB16 JCSG+ Qiagen 130720  96 Sparse Matrix (pH 4.0-10.0)
11 LMB17 Morpheus screen Molecular Dimensions MD1-46 96 Grid screen including mixes of additives and cryoprotected conditions (pH 6.5-8.5)
12 LMB20 Morpheus II screen Molecular Dimensions MD1-91 96 Grid screen including mixes of additives (heavy atoms) and cryoprotected conditions (pH 6.5-8.5)
13 LMB21 LMB crystallisation screen Molecular Dimensions MD1-98 96 Sparse Matrix including conditions from LMB publications.
14 LMB23 Morpheus FUSION Molecular Dimensions MD1-129  96 Incomplete factorial including mixes of additives from the 3 Morpheus screens, cryoprotected conditions, pH 6.5-8.5.
15 LMB24 Helix Molecular Dimensions MD1-689  96 Sparse matrix optimised for DNA, RNA or DNA/RNA hybrids.
16 LMB23 CSIRO organics Molecular Dimensions MD1-150 96 Sparse matrix integrating various organic compounds and polymers as precipitants (pH range 4.2 - 9.5)
17 LMB24 MORPHEUS Z n/a n/a n/a Morpheus grid screen formulation with acidic pH range (4.5-7.5) and upgraded additive mixes.
* Catalogue number for 10 ml, tube format.

Additive screening kits frozen in low profile deep-well blocks.

kit supplier Cat No conditions basic description
Morpheus additive screen Molecular Dimensions MD1-93 96 All the single reagents used to formulate the initial screens Morpheus I and II.
Morpheus-Fusion additive screen Molecular Dimensions MD1-135-FX 96 All the mixes of additives found in the initial screen (same layout).
Silver Bullet Hampton Research HR2-096 96 Mixes of 2-20 small molecules buffered in 0.02 M HEPES sodium pH 6.8.
Detergent screen Hampton Research HR2-407 96 96 mild detergent reagents for use in protein solubility and crystallization studies.
Buffer screen Molecular Dimensions 132983-8UL 48 Buffer gradient (Nextal) pH 2.2-11.6 Nextal. Frozen plates (additives in the first half only).
ANGSTROM Molecular Dimensions MD1-135-FX 96 30 different polyols at 3 different conc..

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Last Updated 2/12/15