MRC-LMB Crystallisation Facility: presentations and other information

Presentations

• Webinars IUCr (2017):
  • Formulation of the MORPHEUS protein crystallization screens (LMB 17, 20 and 22)
  • MORPHEUS: preparation of concentration gradients (Optimization according to the 4-corner method)
• Poster Additive screening.
• Poster Protein Crystallisation.
• Seminar ACA 65 (2015) Innovative protein crystallisation screens.

Other

• Solubility screen (Lamers’ lab wiki-page).
• Terese Bergfors tutorials starting with "Appearances can be deceiving!".
• Movie about Protein Crystallisation (M.A. Dessau & Y. Modis).
• A typical day at the crystallisation facility
• A Dong et al. (2007) In situ proteolysis for protein crystallisation and structure determination. Nat Methods 12, 1019-1021.
• A D'Arcy et al. (2007) An automated microseed matrix-screening method for protein crystallisation. Acta D63, 550-554.
• A Cudney et al. (1994) Screening and optimization strategies for macromolecular crystal growth. Acta D50, 414-423.

Movie of the Crystallisation Facility.

Acknowledgments

We thank Paul Hart (IT) for preparing the LMB screen database web pages and Matthew Fry (Visual Aids) for help with the Crystallisation Facility web pages. Wewould also like to thank Tom Pratt (IT), Tony Warne, Pat Edward (Structural determination of trans-membrane proteins) members of the Mechanical Workshop, Stores and Estates & Facilities. Fusinita van den Ent (The bacterial cytoskeleton) helped with the 4-corner solution worksheets. Karen Law (MRCT) helped with the commercialisation of our innovations.

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Last Updated 2/12/15