MRC-LMB Crystallisation facility: Automated protocols

Tecan EVO platform: preparing initial screens (Falcon tubes into MRC plates)

Fully automated system based on the EVO (Tecan) (SN:1109006508) integrating Carousel and Sealer RS282 (Brandel).

Setting drops (adding protein to plates)

Requirements for the main programs on the MOSQUITO (TTP Labtech).

• Support: MosquitoSupport@ttplabtech.com
• Mosquito 1 (cold-room 1S230); SN: 1510.
• Mosquito 2; SN: LCP-116.
• Mosquito 3: SN: LCP-604.

Main protocol for Hamilton LABSTAR fully automated system (System 2) (Hamilton Robotics) integrating MOSQUITO and sealer.

• Support Hamilton (Mitchell Stuart): Mitchell@hamiltonrobotics.com; SN: 5079.

Preparing optimisation screens (4-corner protocol)

Main protocol on the DRAGONFLY (TTP Labtech).

• Support: Dragonflysupport@ttplabtech.com
• Dragonfly 1 (10-unit head); SN: DF-001.
• Dragonfly 2 (5-unit head); SN: DF-010.

Additional robots

More about our automated protocols

For more detailed information about our automated protocols and references, please see the two publications below.

Fundamentals: D. Stock, O. Perisic, J. Löwe (2015) Progress in Biophysics Robotic nanolitre protein crystallisation at the MRC Laboratory of Molecular Biology

More recent paper: F. Gorrec, J. Löwe (2018) Journal of visualized experiments Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Note: The LabSTAR (Hamilton) is not anymore used as a fully automated platform to set-up plates.

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Last Updated 16/04/26