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RELION

RELION (for REgularised LIkelihood OptimisatioN) is a stand-alone computer program for Maximum A Posteriori refinement of (multiple) 3D reconstructions or 2D class averages in cryo-electron microscopy. It is developed in the research group of Sjors Scheres at the MRC Laboratory of Molecular Biology. The underlying theory of MAP refinement is given in Scheres (2011), submitted. If RELION is useful in your work, please cite this paper! Thank you.

Getting started

• Download & install
• Conventions
• Running RELION

How To

• Preprocess images
• Prepare input files
• Calculate 2D class averages
• Classify structural heterogeneity in 3D
• Refine a structure to high-resolution