MRC-LMB Crystallisation facility: Automated protocols

More details can be found in the Journal of Visualized Experiments (JoVE): Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology

Tecan EVO platform: preparing initial screens (Falcon tubes into MRC plates)

Main protocol for Tecan EVO fully automated system (System 1) integrating Sealer (Brandel) and ink-jet printer (Markem Imaje).

• Support Tecan: helpdesk-uk@tecan.com; SN: 1109006508.
• Support Brandel: George Stephens; george@alphabiotech.co.uk; Model: RS282, SN: RPS-3112S.
• Support Markem Imaje: 0161 333 8500; Model: 9232; SN: FR13360201.

Setting drops (adding protein to plates)

Requirements for the main programs on the MOSQUITO (TTP Labtech).

Two main protocols for additive screening.

• Support: MosquitoSupport@ttplabtech.com
• Mosquito 1 (cold-room 1S230); SN: 1510.
• Mosquito 2; SN: LCP-116.
• Mosquito 3: SN: LCP-604.

Main protocol for Hamilton LABSTAR fully automated system (System 2) (Hamilton Robotics) integrating MOSQUITO and sealer.

• Support Hamilton (Mitchell Stuart): Mitchell@hamiltonrobotics.com; SN: 5079.

Preparing optimisation screens (4-corner protocol)

Main protocol on the DRAGONFLY (TTP Labtech).

• Support: Dragonflysupport@ttplabtech.com
• Dragonfly 1 (10-unit head); SN: DF-001.
• Dragonfly 2 (5-unit head); SN: DF-010.

Manual for GENESIS (Tecan, all formats of plates including non-SBS)

• Support Tecan: helpdesk-uk@tecan.com; SN: 5069.

MINSTREL imager (Rigaku) in cold room 1S230 (4 degrees C)

Please contact a responsive for Robotics when using the Imager and the corresponding web-based interface CrystalTrack Web for the first time.

• Support: +44 1732 765 008; SN imager: 447; SN gallery/carousel: 468.

Contacts

Fabrice Gorrec, room 2N155, (+44 1223 26) 7807, fgorrec@mrc-lmb.cam.ac.uk

alternatively:

Jan Löwe, room 2N159, (+44 1223 26) 7064, jyl@mrc-lmb.cam.ac.uk

Olga Perisic, lab 2S069, (+44 1223 26) 7893, op1@mrc-lmb.cam.ac.uk

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Last Updated 30/1/18