In the transfected epsinR cell in the image on the left, only the perinuclear puncta significantly co-localise with AP1 and not with internalised transferrin. Endogenous epsinR and overexpressed epsinR do not overlap with overexpressed epsin1 (see link), but there is considerable overlap with TGN markers (see below). With higher levels of epsinR expression there are fewer larger puncta (click here for examples).

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