Andriko von Kügelgen

I studied biochemistry in Tübingen after which I moved to the University of Oxford where I joined Tanmay Bharat’s lab for my PhD. During my PhD studies I investigated archaeal and bacterial cell surface proteins using cryo electron tomography and subtomogram averaging.

I am interested in studying the interactions of archaea and bacteria in complex microbial communities within biofilms.

Publications

Selected publications:

1. von Kügelgen, A., Cassidy, C.K., van Dorst, S., Pagani, L.L., Batters, C., Ford, Z., Löwe, J., Alva, V., Stansfeld, P.J., and Bharat, T.A.M., “Membraneless channels sieve cations in ammonia-oxidizing marine archaea”. Nature, (2024). 630(8015): p. 230-236.

2. von Kügelgen, A., Tang, H., Hardy, G.G., Kureisaite-Ciziene, D., Brun, Y.V., Stansfeld, P.J., Robinson, C.V., and Bharat, T.A.M., “In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer”. Cell, (2020). 180(2): p. 348-358 e15.

3. von Kügelgen, A., Alva, V., and Bharat, T.A.M., “Complete atomic structure of a native archaeal cell surface”. Cell Reports, (2021). 37(8): p. 110052.

4. von Kügelgen, A., van Dorst, S., Alva, V., and Bharat, T.A.M., “A multidomain connector links the outer membrane and cell wall in phylogenetically deep-branching bacteria”. PNAS, (2022). 119(33): p. e2203156119.

5. von Kügelgen, A., van Dorst, S., Yamashita, K., Sexton, D.L., Tocheva, E.I., Murshudov, G., Alva, V., and Bharat, T.A.M., “Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacterium Deinococcus radiodurans“. PNAS, (2023). 120(16): p. e2215808120.

All publications:

6. Herdman, M.*, von Kügelgen, A.*, Kureisaite-Ciziene, D., Duman, R., El Omari, K., Garman, E.F., Kjaer, A., Kolokouris, D., Lowe, J., Wagner, A., Stansfeld, P.J., and Bharat, T.A.M., “High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells”. Structure, (2022). 30(2): p. 215-228 e5.

7. Tarafder, A.K., von Kügelgen, A., Mellul, A.J., Schulze, U., Aarts, D., and Bharat, T.A.M., “Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria”. PNAS, (2020). 117(9): p. 4724-4731.

8. Chorev, D.S., Tang, H., Rouse, S.L., Bolla, J.R., von Kügelgen, A., Baker, L.A., Wu, D., Gault, J., Grünewald, K., Bharat, T.A.M., Matthews, S.J., and Robinson, C.V., “The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes”. Nature Protocol, (2020). 15(5): p. 1690-1706.

9. Bharat, T.A.M., von Kügelgen, A., and Alva, V., “Molecular Logic of Prokaryotic Surface Layer Structures”. Trends in Microbiology, (2021). 29(5): p. 405-415.

10. Krause, S.*, Gfrerer, S.*, von Kügelgen, A., Reuse, C., Dombrowski, N., Villanueva, L., Bunk, B., Sproer, C., Neu, T.R., Kuhlicke, U., Schmidt-Hohagen, K., Hiller, K., Bharat, T.A.M., Rachel, R., Spang, A., and Gescher, J., “The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmataleshost”. Nature Communications, (2022). 13(1): p. 1735.

11. Böhning, J., Dobbelstein, A.W. *, Sulkowski, N.*, Eilers, K., von Kügelgen, A., Tarafder, A.K., Peak-Chew, S.Y., Skehel, M., Alva, V., Filloux, A., and Bharat, T.A.M., “Architecture of the biofilm-associated archaic Chaperone-Usher pilus CupE from Pseudomonas aeruginosa“. PLoS Pathogens, (2023). 19(4): p. e1011177.

12. Zivanov, J.*, Otón, J.*, Ke, Z., von Kügelgen, A., Pyle, E., Qu, K., Morado, D., Castano-Diez, D., Zanetti, G., Bharat, T.A.M., Briggs, J.A.G., and Scheres, S.H.W., “A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0”. eLife, (2022). 11.

13. Herdman, M., Isbilir, B., von Kügelgen, A., Schulze, U., Wainman, A., and Bharat, T.A.M., “Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus”. Nature Communications, (2024). 15(1): p. 3355.

14. Burt, A.*, Toader, B.*, Warshamanage, R., von Kügelgen, A., Pyle, E., Zivanov, J., Kimanius, D., Bharat, T.A.M., and Scheres, S.H.W., “An image processing pipeline for electron cryo-tomography in RELION-5”. FEBS Open Bio, (2024).

15. Hamm, J.N. *, Liao, Y.*, von Kügelgen, A., Dombrowski, N., Landers, E., Brownlee, C., Johansson, E.M.V., Whan, R.M., Baker, M.A.B., Baum, B., Bharat, T.A.M., Duggin, I.G., Spang, A., and Cavicchioli, R., “The parasitic lifestyle of an archaeal symbiont”. Nature Communications, (2024). 15(1): p. 6449.