Pubmed articles are listed below. For preprints and other contributions, please see Tanmay Bharat’s Google Scholar page.
2025
- Lindell, A.E., Grießhammer, A., Michaelis, L., Papagiannidis, D., Ochner, H., Kamrad, S., Guan, R., Blasche, S., Ventimiglia, L.N., Ramachandran, B., Ozgur, H., Zelezniak, A., Beristain-Covarrubias, N., Yam-Puc, J.C., Roux, I., Barron, L.P., Richardson, A.K., Martin, M.G., Benes, V., Morone, N., Thaventhiran, J.E.D., Bharat, T.A.M., Savitski, M.M., Maier, L., Patil, K.R. (2025 )
Human gut bacteria bioaccumulate per- and polyfluoroalkyl substances.
Nat Microbiol 10(7): 1630-1647 - Foo, S., Caspy, I., Cezanne, A., Bharat, T.A.M., Baum, B. (2025 )
A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon.
Proc Natl Acad Sci U S A 122(25): e2501044122 - Böhning, J., Graham, M., Coureuil, M., Tarafder, A.K., Meyer, J., Nassif, X., Bille, E., Bharat, T.A.M. (2025 )
Structure of the virulence-associated filamentous bacteriophage MDAΦ.
Proc Natl Acad Sci U S A 122(25): e2420157122 - Fountain, A.J., Böhning, J., McLaughlin, S.H., Morgan, T.E., Edelstein, P.H., Troll, M., Lamers, M.H., Bharat, T.A.M., Luisi, B.F., Ramakrishnan, L. (2025 )
Structural and functional analysis of the MmpS5L5 efflux pump presages a pathway to increased bedaquiline resistance.
bioRxiv [Epub ahead of print] - Isbilir, B., Yeates, A., Alva, V., Bharat, T.A.M. (2025 )
Mapping the ultrastructural topology of the corynebacterial cell surface.
PLoS Biol 23(4): e3003130 - Rados, T., Leland, O.S., Escudeiro, P., Mallon, J., Andre, K., Caspy, I., von Kügelgen, A., Stolovicki, E., Nguyen, S., Patop, I.L., Rangel, L.T., Kadener, S., Renner, L.D., Thiel, V., Soen, Y., Bharat, T.A.M., Alva, V., Bisson, A. (2025 )
Tissue-like multicellular development triggered by mechanical compression in archaea.
Science 388(6742): 109-115 - Caspy, I., Wang, Z., Bharat, T.A.M. (2025 )
Structural biology inside multicellular specimens using electron cryotomography.
Q Rev Biophys 58: e6 - Ochner, H., Bharat, T.A.M. (2025 )
Cryoelectron microscopy with elemental sensitivity.
Structure 33(1): 13-14
2024
- Smith, O.E.R., Bharat, T.A.M. (2024 )
Architectural dissection of adhesive bacterial cell surface appendages from a "molecular machines" viewpoint.
J Bacteriol 206(12): e0029024 - Ochner, H., Böhning, J., Wang, Z., Tarafder, A.K., Caspy, I., Bharat, T.A.M. (2024 )
Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus.
PLoS Pathog 20(12): e1012773 - Burt, A., Toader, B., Warshamanage, R., von Kügelgen, A., Pyle, E., Zivanov, J., Kimanius, D., Bharat, T.A.M., Scheres, S.H.W. (2024 )
An image processing pipeline for electron cryo-tomography in RELION-5.
FEBS Open Bio 14(11): 1788-1804 - Wang, Z., Bharat, T.A.M. (2024)
Serial lift-out for in situ structural biology of multicellular specimens.
Nat Methods 21(9): 1587-1588 - Hamm, J.N., Liao, Y., von Kügelgen, A., Dombrowski, N., Landers, E., Brownlee, C., Johansson, E.M.V., Whan, R.M., Baker, M.A.B., Baum, B., Bharat, T.A.M., Duggin, I.G., Spang, A., Cavicchioli, R. (2024 )
The parasitic lifestyle of an archaeal symbiont.
Nat Commun 15(1): 6449 - von Kügelgen, A., Cassidy, C.K., van Dorst, S., Pagani, L.L., Batters, C., Ford, Z., Löwe, J., Alva, V., Stansfeld, P.J., Bharat, T.A.M. (2024 )
Membraneless channels sieve cations in ammonia-oxidizing marine archaea.
Nature 630(8015): 230-236 - Herdman, M., Isbilir, B., von Kügelgen, A., Schulze, U., Wainman, A., Bharat, T.A.M. (2024 )
Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.
Nat Commun 15(1): 3355 - Böhning, J., Tarafder, A.K., Bharat, T.A.M. (2024 )
The role of filamentous matrix molecules in shaping the architecture and emergent properties of bacterial biofilms.
Biochem J 481(4): 245-263 - Esser, T.K., Böhning, J., Önür, A., Chinthapalli, D.K., Eriksson, L., Grabarics, M., Fremdling, P., Konijnenberg, A., Makarov, A., Botman, A., Peter, C., Benesch, J.L.P., Robinson, C.V., Gault, J., Baker, L., Bharat, T.A.M., Rauschenbach, S. (2024 )
Cryo-EM of soft-landed β-galactosidase: Gas-phase and native structures are remarkably similar.
Sci Adv 10(7): eadl4628
2023
- Bharat, T.A.M., Tocheva, E.I., Alva, V. (2023 )
The cell envelope architecture of Deinococcus: HPI forms the S-layer and SlpA tethers the outer membrane to peptidoglycan.
Proc Natl Acad Sci U S A 120(51): e2305338120 - Böhning, J., Graham, M., Letham, S.C., Davis, L.K., Schulze, U., Stansfeld, P.J., Corey, R.A., Pearce, P., Tarafder, A.K., Bharat, T.A.M. (2023 )
Biophysical basis of filamentous phage tactoid-mediated antibiotic tolerance in P. aeruginosa.
Nat Commun 14(1): 8429 - Ochner, H., Bharat, T.A.M. (2023 )
Charting the molecular landscape of the cell.
Structure 31(11): 1297-1305 - von Kügelgen, A., van Dorst, S., Yamashita, K., Sexton, D.L., Tocheva, E.I., Murshudov, G., Alva, V., Bharat, T.A.M. (2023 )
Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacterium .
Proc Natl Acad Sci U S A 120(16): e2215808120 - Böhning, J., Dobbelstein, A.W., Sulkowski, N., Eilers, K., von Kügelgen, A., Tarafder, A.K., Peak-Chew, S.Y., Skehel, M., Alva, V., Filloux, A., Bharat, T.A.M. (2023 )
Architecture of the biofilm-associated archaic Chaperone-Usher pilus CupE from Pseudomonas aeruginosa.
PLoS Pathog 19(4): e1011177 - Smith, O.E.R., Bharat, T.A.M. (2023)
Seeing the wood for the trees.
Nat Methods 20(2): 183-184
2022
- Zivanov, J., Otón, J., Ke, Z., von Kügelgen, A., Pyle, E., Qu, K., Morado, D., Castaño-Díez, D., Zanetti, G., Bharat, T.A.M., Briggs, J.A.G., Scheres, S.H.W. (2022 )
A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0.
Elife 11 - Böhning, J., Ghrayeb, M., Pedebos, C., Abbas, D.K., Khalid, S., Chai, L., Bharat, T.A.M. (2022 )
Donor-strand exchange drives assembly of the TasA scaffold in Bacillus subtilis biofilms.
Nat Commun 13(1): 7082 - Esser, T.K., Böhning, J., Fremdling, P., Bharat, T., Gault, J., Rauschenbach, S. (2022 )
Cryo-EM samples of gas-phase purified protein assemblies using native electrospray ion-beam deposition.
Faraday Discuss 240(0): 67-80 - Esser, T.K., Böhning, J., Fremdling, P., Agasid, M.T., Costin, A., Fort, K., Konijnenberg, A., Gilbert, J.D., Bahm, A., Makarov, A., Robinson, C.V., Benesch, J.L.P., Baker, L., Bharat, T.A.M., Gault, J., Rauschenbach, S. (2022 )
Mass-selective and ice-free electron cryomicroscopy protein sample preparation via native electrospray ion-beam deposition.
PNAS Nexus 1(4): pgac153 - von Kügelgen, A., van Dorst, S., Alva, V., Bharat, T.A.M. (2022 )
A multidomain connector links the outer membrane and cell wall in phylogenetically deep-branching bacteria.
Proc Natl Acad Sci U S A 119(33): e2203156119 - Krause, S., Gfrerer, S., von Kügelgen, A., Reuse, C., Dombrowski, N., Villanueva, L., Bunk, B., Spröer, C., Neu, T.R., Kuhlicke, U., Schmidt-Hohagen, K., Hiller, K., Bharat, T.A.M., Rachel, R., Spang, A., Gescher, J. (2022 )
The importance of biofilm formation for cultivation of a Micrarchaeon and its interactions with its Thermoplasmatales host.
Nat Commun 13(1): 1735 - Böhning, J., Bharat, T.A.M., Collins, S.M. (2022 )
Compressed sensing for electron cryotomography and high-resolution subtomogram averaging of biological specimens.
Structure 30(3): 408-417.e4 - Herdman, M., von Kügelgen, A., Kureisaite-Ciziene, D., Duman, R., El Omari, K., Garman, E.F., Kjaer, A., Kolokouris, D., Löwe, J., Wagner, A., Stansfeld, P.J., Bharat, T.A.M. (2022 )
High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.
Structure 30(2): 215-228.e5
2021
- von Kügelgen, A., Alva, V., Bharat, T.A.M. (2021 )
Complete atomic structure of a native archaeal cell surface.
Cell Rep 37(8): 110052 - Melia, C.E., Bolla, J.R., Katharios-Lanwermeyer, S., Mihaylov, D.B., Hoffmann, P.C., Huo, J., Wozny, M.R., Elfari, L.M., Böhning, J., Morgan, A.N., Hitchman, C.J., Owens, R.J., Robinson, C.V., O'Toole, G.A., Bharat, T.A.M. (2021 )
Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition.
Proc Natl Acad Sci U S A 118(31) - Böhning, J., Bharat, T.A.M. (2021)
Impressions of expression: bringing structure to the cell.
Nat Rev Microbiol 19(6): 346 - Bharat, T.A.M., von Kügelgen, A., Alva, V. (2021 )
Molecular Logic of Prokaryotic Surface Layer Structures.
Trends Microbiol 29(5): 405-415 - Böhning, J., Bharat, T.A.M. (2021 )
Towards high-throughput in situ structural biology using electron cryotomography.
Prog Biophys Mol Biol 160: 97-103
2020
- Letham, S.C., Bharat, T.A.M. (2020)
Illuminating the dynamics of biofilms.
Nat Rev Microbiol 18(10): 544 - Djeghloul, D., Patel, B., Kramer, H., Dimond, A., Whilding, C., Brown, K., Kohler, A.C., Feytout, A., Veland, N., Elliott, J., Bharat, T.A.M., Tarafder, A.K., Löwe, J., Ng, B.L., Guo, Y., Guy, J., Huseyin, M.K., Klose, R.J., Merkenschlager, M., Fisher, A.G. (2020 )
Identifying proteins bound to native mitotic ESC chromosomes reveals chromatin repressors are important for compaction.
Nat Commun 11(1): 4118 - Chorev, D.S., Tang, H., Rouse, S.L., Bolla, J.R., von Kügelgen, A., Baker, L.A., Wu, D., Gault, J., Grünewald, K., Bharat, T.A.M., Matthews, S.J., Robinson, C.V. (2020 )
The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes.
Nat Protoc 15(5): 1690-1706 - Tarafder, A.K., von Kügelgen, A., Mellul, A.J., Schulze, U., Aarts, D.G.A.L., Bharat, T.A.M. (2020 )
Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria.
Proc Natl Acad Sci U S A 117(9): 4724-4731 - von Kügelgen, A., Tang, H., Hardy, G.G., Kureisaite-Ciziene, D., Brun, Y.V., Stansfeld, P.J., Robinson, C.V., Bharat, T.A.M. (2020 )
In Situ Structure of an Intact Lipopolysaccharide-Bound Bacterial Surface Layer.
Cell 180(2): 348-358.e15
2019
- Hoffmann, P.C., Bharat, T.A.M., Wozny, M.R., Boulanger, J., Miller, E.A., Kukulski, W. (2019 )
Tricalbins Contribute to Cellular Lipid Flux and Form Curved ER-PM Contacts that Are Bridged by Rod-Shaped Structures.
Dev Cell 51(4): 488-502.e8 - Sulkowski, N.I., Hardy, G.G., Brun, Y.V., Bharat, T.A.M. (2019 )
A Multiprotein Complex Anchors Adhesive Holdfast at the Outer Membrane of Caulobacter crescentus.
J Bacteriol 201(18)
2018
- Melia, C.E., Bharat, T.A.M. (2018 )
Locating macromolecules and determining structures inside bacterial cells using electron cryotomography.
Biochim Biophys Acta Proteins Proteom 1866(9): 973-981 - Bharat, T.A.M., Hoffmann, P.C., Kukulski, W. (2018 )
Correlative Microscopy of Vitreous Sections Provides Insights into BAR-Domain Organization In Situ.
Structure 26(6): 879-886.e3 - Fernandez, J.J., Li, S., Bharat, T.A.M., Agard, D.A. (2018 )
Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.
J Struct Biol 202(3): 200-209
2017
- Deng, X., Fink, G., Bharat, T.A.M., He, S., Kureisaite-Ciziene, D., Löwe, J. (2017 )
Four-stranded mini microtubules formed by BtubAB show dynamic instability.
Proc Natl Acad Sci U S A 114(29): E5950-E5958 - Bharat, T.A.M., Kureisaite-Ciziene, D., Hardy, G.G., Yu, E.W., Devant, J.M., Hagen, W.J.H., Brun, Y.V., Briggs, J.A.G., Löwe, J. (2017 )
Structure of the hexagonal surface layer on Caulobacter crescentus cells.
Nat Microbiol 2: 17059
2016
- Bharat, T.A., Scheres, S.H. (2016 )
Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION.
Nat Protoc 11(11): 2054-65 - Martin, T.G., Bharat, T.A., Joerger, A.C., Bai, X.C., Praetorius, F., Fersht, A.R., Dietz, H., Scheres, S.H. (2016 )
Design of a molecular support for cryo-EM structure determination.
Proc Natl Acad Sci U S A 113(47): E7456-E7463
2015
- Bharat, T.A.M., Russo, C.J., Löwe, J., Passmore, L.A., Scheres, S.H.W. (2015 )
Advances in Single-Particle Electron Cryomicroscopy Structure Determination applied to Sub-tomogram Averaging.
Structure 23(9): 1743-1753 - Bharat, T.A., Murshudov, G.N., Sachse, C., Löwe, J. (2015 )
Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles.
Nature 523(7558): 106-10 - Fromm, S.A., Bharat, T.A., Jakobi, A.J., Hagen, W.J., Sachse, C. (2015 )
Seeing tobacco mosaic virus through direct electron detectors.
J Struct Biol 189(2): 87-97
2014
- Szwedziak, P., Wang, Q., Bharat, T.A., Tsim, M., Löwe, J. (2014 )
Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell division.
Elife 3: e04601 - Davey, N.E., Satagopam, V.P., Santiago-Mozos, S., Villacorta-Martin, C., Bharat, T.A., Schneider, R., Briggs, J.A. (2014 )
The HIV mutation browser: a resource for human immunodeficiency virus mutagenesis and polymorphism data.
PLoS Comput Biol 10(12): e1003951 - Bharat, T.A., Castillo Menendez, L.R., Hagen, W.J., Lux, V., Igonet, S., Schorb, M., Schur, F.K., Kräusslich, H.G., Briggs, J.A. (2014 )
Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly.
Proc Natl Acad Sci U S A 111(22): 8233-8 - van den Ent, F., Izoré, T., Bharat, T.A., Johnson, C.M., Löwe, J. (2014 )
Bacterial actin MreB forms antiparallel double filaments.
Elife 3: e02634 - Bharat, T.A., Malsam, J., Hagen, W.J., Scheutzow, A., Söllner, T.H., Briggs, J.A. (2014 )
SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calcium-triggered fusion.
EMBO Rep 15(3): 308-14
2013
- Bharat, T.A., Zbaida, D., Eisenstein, M., Frankenstein, Z., Mehlman, T., Weiner, L., Sorzano, C.O., Barak, Y., Albeck, S., Briggs, J.A., Wolf, S.G., Elbaum, M. (2013 )
Variable internal flexibility characterizes the helical capsid formed by agrobacterium VirE2 protein on single-stranded DNA.
Structure 21(7): 1158-67
2012
- Steringer, J.P., Bleicken, S., Andreas, H., Zacherl, S., Laussmann, M., Temmerman, K., Contreras, F.X., Bharat, T.A., Lechner, J., Müller, H.M., Briggs, J.A., García-Sáez, A.J., Nickel, W. (2012 )
Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-dependent oligomerization of fibroblast growth factor 2 (FGF2) triggers the formation of a lipidic membrane pore implicated in unconventional secretion.
J Biol Chem 287(33): 27659-69 - Malsam, J., Parisotto, D., Bharat, T.A., Scheutzow, A., Krause, J.M., Briggs, J.A., Söllner, T.H. (2012 )
Complexin arrests a pool of docked vesicles for fast Ca2+-dependent release.
EMBO J 31(15): 3270-81 - Bharat, T.A., Davey, N.E., Ulbrich, P., Riches, J.D., de Marco, A., Rumlova, M., Sachse, C., Ruml, T., Briggs, J.A. (2012 )
Structure of the immature retroviral capsid at 8 Å resolution by cryo-electron microscopy.
Nature 487(7407): 385-9 - Bharat, T.A., Noda, T., Riches, J.D., Kraehling, V., Kolesnikova, L., Becker, S., Kawaoka, Y., Briggs, J.A. (2012 )
Structural dissection of Ebola virus and its assembly determinants using cryo-electron tomography.
Proc Natl Acad Sci U S A 109(11): 4275-80
2011
- Bharat, T.A., Riches, J.D., Kolesnikova, L., Welsch, S., Krähling, V., Davey, N., Parsy, M.L., Becker, S., Briggs, J.A. (2011 )
Cryo-electron tomography of Marburg virus particles and their morphogenesis within infected cells.
PLoS Biol 9(11): e1001196
2008
- Bharat, T.A., Eisenbeis, S., Zeth, K., Höcker, B. (2008 )
A beta alpha-barrel built by the combination of fragments from different folds.
Proc Natl Acad Sci U S A 105(29): 9942-7