Molecular basis for membrane remodelling and organization

Invited Speakers (in alphabetical order) and a selection 3 recent papers each





Antonny, Bruno, CNRS Valbonne, France:

Membrane curvature and COP coated vesicle budding
  1. ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions. Ambroggio E, Sorre B, Bassereau P, Goud B, Manneville JB, Antonny B. EMBO J. 2009, Nov19.
  2. Asymmetric tethering of flat and curved lipid membranes by a golgin. Drin G, Morello V, Casella JF, Gounon P, Antonny B. Science. 2008 May 2;320(5876):670-3.
  3. A general amphipathic alpha-helical motif for sensing membrane curvature. Drin G, Casella JF, Gautier R, Boehmer T, Schwartz TU, Antonny B. Nature Struct Mol Biol. 2007 Feb;14(2):138-46.

Aspenström, Pontus, Karolinska Institute, Stockholm, Sweden:

Signalling, actin and membrane dynamics
  1. Members of the CIP4 family of proteins participate in the regulation of platelet-derived growth factor receptor-beta-dependent actin reorganization and migration. Toguchi M, Richnau N, Ruusala A, Aspenström P. Biol Cell. 2009 Nov 12.
  2. Nck adapters are involved in the formation of dorsal ruffles, cell migration, and Rho signaling downstream of the platelet-derived growth factor beta receptor. Ruusala A, Pawson T, Heldin CH, Aspenström P. J Biol Chem. 2008 Oct 31;283(44):30034-44.
  3. The atypical Rho GTPase Wrch1 collaborates with the nonreceptor tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics. Ruusala A, Aspenström P. Mol Cell Biol. 2008 Mar;28(5):1802-14.

Bassereau, Patricia, Institute Curie, Paris:

Dynamics of cell membranes explored by biophysical techniques
  1. Curvature-driven lipid sorting needs proximity to a demixing point and is aided by proteins. Sorre B, Callan-Jones A, Manneville JB, Nassoy P, Joanny JF, Prost J, Goud B, Bassereau P. Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):5622-6.
  2. Membrane tension lowering induced by protein activity. El Alaoui Faris MD, Lacoste D, Pécréaux J, Joanny JF, Prost J, Bassereau P. Phys Rev Lett. 2009 Jan 23;102(3):038102.
  3. Shiga toxin induces tubular membrane invaginations for its uptake into cells. Römer W, Berland L, Chambon V, Gaus K, Windschiegl B, Tenza D, Aly MR, Fraisier V, Florent JC, Perrais D, Lamaze C, Raposo G, Steinem C, Sens P, Bassereau P, Johannes L. Nature. 2007 Nov 29;450(7170):670-5.

Carlier, Marie-France, Gif-sur-Yvette, Paris:

Cytoskeleton dynamics and cell motility
  1. How tropomyosin regulates lamellipodial actin-based motility: a combined biochemical and reconstituted motility approach. Bugyi B, Didry D, Carlier MF. EMBO J. 2009 Nov 5
  2. Analysis of the function of Spire in actin assembly and its synergy with formin and profilin. Bosch M, Le KH, Bugyi B, Correia JJ, Renault L, Carlier MF. Mol Cell. 2007 Nov 30;28(4):555-68.
  3. How ATP hydrolysis controls filament assembly from profilin-actin: implication for formin processivity. Romero S, Didry D, Larquet E, Boisset N, Pantaloni D, Carlier MF. J Biol Chem. 2007 Mar 16;282(11):8435-45

Chernomordik, Leonid, NICHD, NIH, Bethesda, USA:

Viral infection mechanisms and intermediates of membrane fusion
  1. Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores. Rafikova ER, Melikov K, Ramos C, Dye L, Chernomordik LV. J Biol Chem. 2009 Oct 23;284(43):29847-59.
  2. Cytoskeleton reorganization in influenza hemagglutinin-initiated syncytium formation. Richard JP, Leikina E, Chernomordik LV. Biochim Biophys Acta. 2009 Feb;1788(2):450-7.
  3. Fusion-pore expansion during syncytium formation is restricted by an actin network. Chen A, Leikina E, Melikov K, Podbilewicz B, Kozlov MM, Chernomordik LV. J Cell Sci. 2008 Nov 1;121(Pt 21):3619-28.

Darchen, François, Institut de Biologie Physico-Chimique, Université Paris:

Secretory vesicle docking and fusion, and SNARE protein zippering
  1. A fast mode of membrane fusion dependent on tight SNARE zippering. Bretou M, Anne C, Darchen F. J Neurosci. 2008 Aug 20;28(34):8470-6.
  2. Myosin va mediates docking of secretory granules at the plasma membrane. Desnos C, Huet S, Fanget I, Chapuis C, Böttiger C, Racine V, Sibarita JB, Henry JP, Darchen F. J Neurosci. 2007 Sep 26;27(39):10636-45.
  3. Distinct role of Rab3A and Rab3B in secretory activity of rat melanotrophs. Rupnik M, Kreft M, Nothias F, Grilc S, Bobanovic LK, Johannes L, Kiauta T, Vernier P, Darchen F, Zorec R. Am J Physiol Cell Physiol. 2007 Jan;292(1):C98-105.

De Camilli, Pietro, HHMI, Yale University, USA:

Clathrin-mediated endocytosis: characterization of the molecular components and their functions
  1. A phosphoinositide switch controls the maturation and signaling properties of APPL endosomes. Zoncu R, Perera RM, Balkin DM, Pirruccello M, Toomre D, De Camilli P. Cell. 2009 Mar 20;136(6):1110-21.
  2. Structural basis of membrane invagination by F-BAR domains. Frost A, Perera R, Roux A, Spasov K, Destaing O, Egelman EH, De Camilli P, Unger VM. Cell. 2008 Mar 7;132(5):807-17.
  3. A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, Cremona O, Miesenböck G, Ryan TA, De Camilli P. Science. 2007 Apr 27;316(5824):570-4.

Geyer, Matthias, Dortmund, Germany:

HIV Gag proteins and membrane curvature
  1. HIV-1 Nef membrane association depends on charge, curvature, composition and sequence. Gerlach H, Laumann V, Martens S, Becker CF, Goody RS, Geyer M. Nature Chem Biol. 2010 Jan;6(1):46-53.
  2. Structural insights into the cyclin T1-Tat-TAR RNA transcription activation complex from EIAV. Anand K, Schulte A, Vogel-Bachmayr K, Scheffzek K, Geyer M. Nature Struct Mol Biol. 2008 Dec;15(12):1287-92.
  3. The human formin FHOD1 contains a bipartite structure of FH3 and GTPase-binding domains required for activation. Schulte A, Stolp B, Schönichen A, Pylypenko O, Rak A, Fackler OT, Geyer M. Structure. 2008 Sep 10;16(9):1313-23.

Goud, Bruno, Curie, France:

COP-coated vesicle budding, vesicle trafficking and membrane deformation
  1. COPI coat assembly occurs on liquid-disordered domains and the associated membrane deformations are limited by membrane tension. Manneville JB, Casella JF, Ambroggio E, Gounon P, Bertherat J, Bassereau P, Cartaud J, Antonny B, Goud B. Proc Natl Acad Sci U S A. 2008 Nov 4;105(44):16946-51.
  2. Intracellular transport: from physics to biology. Roux A, Cuvelier D, Bassereau P, Goud B. Ann N Y Acad Sci. 2008 Mar;1123:119-25.
  3. Rab6-interacting protein 1 links Rab6 and Rab11 function. Miserey-Lenkei S, Waharte F, Boulet A, Cuif MH, Tenza D, El Marjou A, Raposo G, Salamero J, Héliot L, Goud B, Monier S. Traffic. 2007 Oct;8(10):1385-403.

Johannes, Ludger, Curie Institute, Paris:

Entry mechanisms for toxins and viruses, and the role of membrane curvature
  1. Actin dynamics drive membrane reorganization and scission in clathrin-independent endocytosis. Römer, W., L.L. Pontani, B. Sorre, C. Rentero, L. Berland, V. Chambon, C. Lamaze, P. Bassereau, C. Sykes, K. Gaus, and L. Johannes. Cell. 2010 140:540-553.
  2. SV40 binding to its receptor, GM1, induces membrane invagination, tubulation, and infection. Ewers, H., W. Römer, A.E. Smith, K. Bacia, S. Dmitrieff, W. Chai, R. Mancini, J. Kartenbeck, V. Chambon, L. Berland, A. Oppenheim, G. Schwarzmann, T. Feizi, P. Schwille, P. Sens, A. Helenius, and L. Johannes. Nat. Cell Biol. 2010 12:11-18.
  3. Shiga toxin induces tubular membrane invaginations for its uptake into cells. Römer, W., L. Berland, V. Chambon, K. Gaus, B. Windschiegl, D. Tenza, M.R. Aly, V. Fraisier, J.-C. Florent, D. Perrais, C. Lamaze, G. Raposo, C. Steinem, P. Sens, P. Bassereau, and L. Johannes. Nature. 2007. 450:670-675.

Kozlov, Misha M, Tel Aviv University, Israel:

Energetic of membrane curvature
  1. Computational model of membrane fission catalyzed by ESCRT-III. Fabrikant G, Lata S, Riches JD, Briggs JA, Weissenhorn W, Kozlov MM. PLoS Comput Biol. 2009 Nov;5(11):e1000575.
  2. Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Hu J, Shibata Y, Voss C, Shemesh T, Li Z, Coughlin M, Kozlov MM, Rapoport TA, Prinz WA. Science. 2008 Feb 29;319(5867):1247-50.
  3. How synaptotagmin promotes membrane fusion. Martens S, Kozlov MM, McMahon HT. Science. 2007 May 25;316(5828):1205-8.

Lappalainen, Pekka, University of Helsinki, Finland:

Filopodia, actin and inverse membrane curvature generation
  1. Defining mechanisms of actin polymerization and depolymerization during dendritic spine morphogenesis. Hotulainen P, Llano O, Smirnov S, Tanhuanpää K, Faix J, Rivera C, Lappalainen P. J Cell Biol. 2009 Apr 20;185(2):323-39.
  2. Molecular mechanisms of membrane deformation by I-BAR domain proteins. Saarikangas J, Zhao H, Pykäläinen A, Laurinmäki P, Mattila PK, Kinnunen PK, Butcher SJ, Lappalainen P. Curr Biol. 2009 Jan 27;19(2):95-107.
  3. Missing-in-metastasis and IRSp53 deform PI(4,5)P2-rich membranes by an inverse BAR domain-like mechanism. Mattila PK, Pykäläinen A, Saarikangas J, Paavilainen VO, Vihinen H, Jokitalo E, Lappalainen P. J Cell Biol. 2007 Mar 26;176(7):953-64.

Langen, Ralf, Zilkha Neurogenetic Institute, Los Angeles, USA:

Biophysical methods for analysing protein-membrane interactions
  1. Structure of membrane-bound alpha-synuclein from site-directed spin labeling and computational refinement. Jao CC, Hegde BG, Chen J, Haworth IS, Langen R. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19666-71.
  2. Structure of alpha-helical membrane-bound human islet amyloid polypeptide and its implications for membrane-mediated misfolding. Apostolidou M, Jayasinghe SA, Langen R. J Biol Chem. 2008 Jun 20;283(25):17205-10.
  3. Structure and analysis of FCHo2 F-BAR domain: a dimerizing and membrane recruitment module that effects membrane curvature. Henne WM, Kent HM, Ford MG, Hegde BG, Daumke O, Butler PJ, Mittal R, Langen R, Evans PR, McMahon HT. Structure. 2007 Jul;15(7):839-52.

Lenne, Pierre-Francois, Marseille, France:

Shapes and mechanics in morphogenesis
  1. Nature and anisotropy of cortical forces orienting Drosophila tissue morphogenesis. Rauzi M, Verant P, Lecuit T, Lenne PF. Nature Cell Biol. 2008 Dec;10(12):1401-10.
  2. A two-tiered mechanism for stabilization and immobilization of E-cadherin. Cavey M, Rauzi M, Lenne PF, Lecuit T. Nature. 2008 Jun 5;453(7196):751-6.
  3. Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis. Lecuit T, Lenne PF. Nature Rev Mol Cell Biol. 2007 Aug;8(8):633-44.

Meresse, Stephane, Marseille, France:

Salmonella infection and maturation, SifA and endosome tubulation
  1. Interaction between the SifA virulence factor and its host target SKIP is essential for Salmonella pathogenesis. Diacovich L, Dumont A, Lafitte D, Soprano E, Guilhon AA, Bignon C, Gorvel JP, Bourne Y, Méresse S. J Biol Chem. 2009 Nov 27;284(48):33151-60.
  2. Pathogenic Bacteria and Dead Cells Are Internalized by a Unique Subset of Peyer's Patch Dendritic Cells That Express Lysozyme. Lelouard H, Henri S, De Bovis B, Mugnier B, Chollat-Namy A, Malissen B, Méresse S, Gorvel JP. Gastroenterology. 2009.
  3. Analysis of kinesin accumulation on Salmonella-containing vacuoles. Dumont A, Schroeder N, Gorvel JP, Méresse S. Methods Mol Biol. 2007;394:275-87.

Molle, Gérard, Lyon, France:

Pore-Forming Toxins
  1. First evidence of the pore-forming properties of a keratin from skin mucus of rainbow trout (Oncorhynchus mykiss, formerly Salmo gairdneri). Molle V, Campagna S, Bessin Y, Ebran N, Saint N, Molle G. Biochem J. 2008 Apr 1;411(1):33-40.
  2. Structure and mechanism of action of the antimicrobial peptide piscidin. Campagna S, Saint N, Molle G, Aumelas A. Biochemistry. 2007 Feb 20;46(7):1771-8.
  3. The N-terminal domain of OmpATb is required for membrane translocation and pore-forming activity in mycobacteria. Alahari A, Saint N, Campagna S, Molle V, Molle G, Kremer L. J Bacteriol. 2007 Sep;189(17):6351-8.

Petit, Christine, Institut Pasteur, Paris:

Hearing, otoferlins and synaptic transmission in the ear
  1. Otoferlin is critical for a highly sensitive and linear calcium-dependent exocytosis at vestibular hair cell ribbon synapses. Dulon D, Safieddine S, Jones SM, Petit C. J Neurosci. 2009 Aug 26;29(34):10474-87.
  2. Stereocilin-deficient mice reveal the origin of cochlear waveform distortions. Verpy E, Weil D, Leibovici M, Goodyear RJ, Hamard G, Houdon C, Lefèvre GM, Hardelin JP, Richardson GP, Avan P, Petit C. Nature. 2008 Nov 13;456(7219):255-8.
  3. Connexin30 deficiency causes instrastrial fluid-blood barrier disruption within the cochlear stria vascularis. Cohen-Salmon M, Regnault B, Cayet N, Caille D, Demuth K, Hardelin JP, Janel N, Meda P, Petit C. Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6229-34.

Rapoport, Tom A, Harvard, Boston, USA:

ER fission and fusion, and protein-translocation
  1. A role for the two-helix finger of the SecA ATPase in protein translocation. Erlandson KJ, Miller SB, Nam Y, Osborne AR, Zimmer J, Rapoport TA. Nature. 2008 Oct 16;455(7215):984-7.
  2. Structure of a complex of the ATPase SecA and the protein-translocation channel. Zimmer J, Nam Y, Rapoport TA. Nature. 2008 Oct 16;455(7215):936-43.
  3. Membrane proteins of the endoplasmic reticulum induce high-curvature tubules. Hu J, Shibata Y, Voss C, Shemesh T, Li Z, Coughlin M, Kozlov MM, Rapoport TA, Prinz WA. Science. 2008 Feb 29;319(5867):1247-50.

Rey, Felix, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France:

Virus structures and cell entry mechanisms
  1. Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of respiratory syncytial virus. Tawar RG, Duquerroy S, Vonrhein C, Varela PF, Damier-Piolle L, Castagné N, MacLellan K, Bedouelle H, Bricogne G, Bhella D, Eléouët JF, Rey FA. Science. 2009 Nov 27;326(5957):1279-83.
  2. The picobirnavirus crystal structure provides functional insights into virion assembly and cell entry. Duquerroy S, Da Costa B, Henry C, Vigouroux A, Libersou S, Lepault J, Navaza J, Delmas B, Rey FA. EMBO J. 2009 Jun 3;28(11):1655-65.
  3. Structure of the prefusion form of the vesicular stomatitis virus glycoprotein G. Roche S, Rey FA, Gaudin Y, Bressanelli S. Science. 2007 Feb 9;315(5813):843-8

Rothman, James E, Yale, New Haven, USA:

SNARE proteins and the mechanism of membrane fusion
  1. Alternative zippering as an on-off switch for SNARE-mediated fusion. Giraudo CG, Garcia-Diaz A, Eng WS, Chen Y, Hendrickson WA, Melia TJ, Rothman JE. Science. 2009 Jan 23;323(5913):512-6.
  2. SNAREpin/Munc18 promotes adhesion and fusion of large vesicles to giant membranes. Tareste D, Shen J, Melia TJ, Rothman JE. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2380-5.
  3. Distinct domains of complexins bind SNARE complexes and clamp fusion in vitro. Giraudo CG, Garcia-Diaz A, Eng WS, Yamamoto A, Melia TJ, Rothman JE. J Biol Chem. 2008 Jul 25;283(30):21211-9.

Stamou, Dimitrios, University of Copenhagen, Denmark:

Fluorescent methods for membrane curvature sensing
  1. Amphipathic motifs in BAR domains are essential for membrane curvature sensing. Bhatia VK, Madsen KL, Bolinger PY, Kunding A, Hedegård P, Gether U, Stamou D. EMBO J. 2009 Nov 4;28(21):3303-14.
  2. How curved membranes recruit amphipathic helices and protein anchoring motifs. Hatzakis NS, Bhatia VK, Larsen J, Madsen KL, Bolinger PY, Kunding AH, Castillo J, Gether U, Hedegård P, Stamou D. Nature Chem Biol. 2009 Nov;5(11):835-41.
  3. A fluorescence-based technique to construct size distributions from single-object measurements: application to the extrusion of lipid vesicles. Kunding AH, Mortensen MW, Christensen SM, Stamou D. Biophys J. 2008 Aug;95(3):1176-88.

Zimmerberg, Joshua, NIH Bethesda, USA:

Mammalian dynamin function
  1. Insulin regulates fusion of GLUT4 vesicles independent of Exo70-mediated tethering. Lizunov VA, Lisinski I, Stenkula K, Zimmerberg J, Cushman SW. J Biol Chem. 2009 Mar 20;284(12):7914-9.
  2. GTPase cycle of dynamin is coupled to membrane squeeze and release, leading to spontaneous fission. Bashkirov PV, Akimov SA, Evseev AI, Schmid SL, Zimmerberg J, Frolov VA. ,Cell. 2008 Dec 26;135(7):1276-86.
  3. Irreversible effect of cysteine protease inhibitors on the release of malaria parasites from infected erythrocytes. Glushakova S, Mazar J, Hohmann-Marriott MF, Hama E, Zimmerberg J. Cell Microbiol. 2009 Jan;11(1):95-105.

Weissenhorn, Winfried, Grenoble, France:

Structures of ESCRT proteins, BAR proteins and membrane curvature
  1. A crescent-shaped ALIX dimer targets ESCRT-III CHMP4 filaments. Pires R, Hartlieb B, Signor L, Schoehn G, Lata S, Roessle M, Moriscot C, Popov S, Hinz A, Jamin M, Boyer V, Sadoul R, Forest E, Svergun DI, Göttlinger HG, Weissenhorn W. Structure. 2009 Jun 10;17(6):843-56.
  2. Helical structures of ESCRT-III are disassembled by VPS4. Lata S, Schoehn G, Jain A, Pires R, Piehler J, Gottlinger HG, Weissenhorn W. Science. 2008 Sep 5;321(5894):1354-7.
  3. Structural basis for autoinhibition of ESCRT-III CHMP3. Lata S, Roessle M, Solomons J, Jamin M, Gottlinger HG, Svergun DI, Weissenhorn W. J Mol Biol. 2008 May 9;378(4):818-27.