Welcome to the Holliger Lab
Recent Publications
Gianni E, Kwok SLY, Wan CJK, Goeij K, Clifton BE, Attwater J & Holliger P (2024) A polymerase ribozyme that can synthesize both itself and its complementary strand. (bioRxiv) Science in press
Attwater J, Augustin TL, Curran JF, Kwok SLY, Ohlendorf L, Gianni E & Holliger P (2025) Trinucleotide substrates under pH-freeze-thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme. Nature Chem, 17, 1129–1137
Porebski BT, Balmforth M, Browne G, Riley A, Jamali K, Fürst MJLJ, Velic M, Buchanan A, Minter R, Vaughan T & Holliger P (2024) Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening. Nature Biomed Eng 8, 214-232
We are working on 1) the synthesis, replication and evolution of entirely synthetic genetic polymers (XNAs) capable of information storage, propagation and evolution, 2) deep screening, an ultra-high-throughput technology that leverages the Illumina HiSeq platform for massively parallel sequencing, display, and global screening of diverse biomolecular interactions, including antibody repertoires and 3) the reconstruction of a key component of the RNA world, an RNA molecule capable of self-replication and mutation and hence, evolution towards ever more efficient self-replication and the potential role of water ice in that process.