EM Monolayer Assay Images

This assay allows us to visualize the early steps of endocytosis by electron microscopy.
See Ford et al, Science 291: 1051-1055 (.pdf) or Higgins and McMahon, TIBS 27: 257-259 (.pdf) for details and examples.
Proteins were injected under a 10% PtdIns(4,5)P2 lipid monolayer, incubated at room temperature, stained by uranyl acetate and visualized by EM. (see schematic technique or detailed protocol)
These are images of EM negatives, scale bars of either 100 nm (40000x images) or 300 nm (20000x images) are visible on the larger images.
The stereo images are best visualized with a separation of 6 cm.

AP180 + Clathrin

Small image (141 kb)

Large image (809 kb)

 Small image (53 kb)

Large image (574 kb)

Epsin + Clathrin
Small stereo image (48 kb)

Large stereo image (552 kb)

 Small image (87 kb)

Large image (751 kb)

 Small image (61 kb)

Large image (775 kb)

AP180 + Epsin + Clathrin

Small image (46 kb)

Large image (496 kb)

 Small image (68 kb)

Large image (641 kb)

Small image (74 kb)

Large image (716 kb)
Small image (76 kb)

Large image (652 kb)
Small image (68 kb)

Large image (646 kb)

 Small image (131 kb)

Large image (713 kb)

AP180 + AP2 + Clathrin

AP180 + Clathrin, platinum shadowed

AP180 + AP2 + Clathrin, platinum shadowed


Other EMs (limited access)

Epsin 1 Home Page
 McMahon Group home page