The Nagai lab was interested in the structure and function of the eukaryotic splicing machine: the spliceosome. They a number of crystal and NMR structures of spliceosome components, and elucidated high resolution cryo-EM structures of the entire spliceosome at different stages of catalysis. These structures provide a framework for dissecting the mechanism of pre-mRNA splicing.
There is also a review article in Crystals written by Adelaine Leung, a former PhD student in Kiyoshi Nagai’s lab and co-authored by other former Nagai lab members. It is a dedication to their friends and mentors Kiyoshi Nagai and Chris Oubridge, who are sorely missed. It summarises the lab’s vision, immense amounts of work and sometimes crazy ideas to unravel and understand the mechanism of splicing using X-ray crystallography before the Cryo-EM revolution.