Publications

Selected publications

(for Full publication list click here)

• Wilkinson, M.E., Charenton, C., Nagai, K. (2020)
  RNA Splicing by the Spliceosome.
  Annu Rev Biochem 89: 359-388
• Plaschka, C., Newman, A.J., Nagai, K. (2019)
  Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast.
  Cold Spring Harb Perspect Biol 11(5)
• Charenton, C., Wilkinson, M.E., Nagai, K. (2019)
  Mechanism of 5' splice site transfer for human spliceosome activation.
  Science 364(6438): 362-367
• Fica, S.M., Oubridge, C., Wilkinson, M.E., Newman, A.J., Nagai, K. (2019)
  A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.
  Science 363(6428): 710-714
• Plaschka, C., Lin, P.C., Charenton, C., Nagai, K. (2018)
  Prespliceosome structure provides insights into spliceosome assembly and regulation.
  Nature 559(7714): 419-422
• Wilkinson, M.E., Fica, S.M., Galej, W.P., Norman, C.M., Newman, A.J., Nagai, K. (2017)
  Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.
  Science 358(6368): 1283-1288
• Fica, S.M., Nagai, K. (2017)
  Cryo-electron microscopy snapshots of the spliceosome: structural insights into a dynamic ribonucleoprotein machine.
  Nat Struct Mol Biol 24(10): 791-799
• Plaschka, C., Lin, P.C., Nagai, K. (2017)
  Structure of a pre-catalytic spliceosome.
  Nature 546(7660): 617-621
• Fica, S.M., Oubridge, C., Galej, W.P., Wilkinson, M.E., Bai, X.C., Newman, A.J., Nagai, K. (2017)
  Structure of a spliceosome remodelled for exon ligation.
  Nature 542(7641): 377-380
• Galej, W.P., Wilkinson, M.E., Fica, S.M., Oubridge, C., Newman, A.J., Nagai, K. (2016)
  Cryo-EM structure of the spliceosome immediately after branching.
  Nature 537(7619): 197-201
• Nguyen, T.H.D., Galej, W.P., Bai, X.C., Oubridge, C., Newman, A.J., Scheres, S.H.W., Nagai, K. (2016)
  Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution.
  Nature 530(7590): 298-302
• Nguyen, T.H., Galej, W.P., Bai, X.C., Savva, C.G., Newman, A.J., Scheres, S.H., Nagai, K. (2015)
  The architecture of the spliceosomal U4/U6.U5 tri-snRNP.
  Nature 523(7558): 47-52
• Kondo, Y., Oubridge, C., van Roon, A.M., Nagai, K. (2015)
  Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition.
  Elife 4
• Nguyen, T.H., Li, J., Galej, W.P., Oshikane, H., Newman, A.J., Nagai, K. (2013)
  Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.
  Structure 21(6): 910-19
• Galej, W.P., Oubridge, C., Newman, A.J., Nagai, K. (2013)
  Crystal structure of Prp8 reveals active site cavity of the spliceosome.
  Nature 493(7434): 638-43
• Leung, A.K., Nagai, K., Li, J. (2011)
  Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis.
  Nature 473(7348): 536-9
• Pomeranz Krummel, D.A., Oubridge, C., Leung, A.K., Li, J., Nagai, K. (2009)
  Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution.
  Nature 458(7237): 475-80

Full publication list

(for selected publications click here)

• 2021
• 2020
• 2019
• 2018
• 2017
• 2016
• 2015
• 2014
• 2013
• 2011
• 2010
• 2009
• 2008
• 2007
• 2006
• 2005
• 2004
• 2003
• 2002
• 2001
• 2000
• 1999
• 1998
• 1996
• 1995
• 1994
• 1993
• 1991
• 1990
• 1988
• 1987

2021

• Wilkinson, M.E., Fica, S.M., Galej, W.P., Nagai, K. (2021)
  Structural basis for conformational equilibrium of the catalytic spliceosome.
  Mol Cell 81(7): 1439-1452.e9
• Strittmatter, L.M., et al. (2021)
  psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation.
  Nat Commun 12(1): 1488

2020

• Wilkinson, M.E., Charenton, C., Nagai, K. (2020)
  RNA Splicing by the Spliceosome.
  Annu Rev Biochem 89: 359-388

2019

• Plaschka, C., Newman, A.J., Nagai, K. (2019)
  Structural Basis of Nuclear pre-mRNA Splicing: Lessons from Yeast.
  Cold Spring Harb Perspect Biol 11(5)
• Charenton, C., Wilkinson, M.E., Nagai, K. (2019)
  Mechanism of 5' splice site transfer for human spliceosome activation.
  Science 364(6438): 362-367
• Fica, S.M., Oubridge, C., Wilkinson, M.E., Newman, A.J., Nagai, K. (2019)
  A human postcatalytic spliceosome structure reveals essential roles of metazoan factors for exon ligation.
  Science 363(6428): 710-714

2018

• Scott, W.G., Nagai, K. (2018)
  Recruiting more proteins to the RNA world.
  Science 362(6415): 644-645
• Plaschka, C., Lin, P.C., Charenton, C., Nagai, K. (2018)
  Prespliceosome structure provides insights into spliceosome assembly and regulation.
  Nature 559(7714): 419-422
• Wilkinson, M.E., Lin, P.C., Plaschka, C., Nagai, K. (2018)
  Cryo-EM Studies of Pre-mRNA Splicing: From Sample Preparation to Model Visualization.
  Annu Rev Biophys 47: 175-199
• Galej, W.P., Toor, N., Newman, A.J., Nagai, K. (2018)
  Molecular Mechanism and Evolution of Nuclear Pre-mRNA and Group II Intron Splicing: Insights from Cryo-Electron Microscopy Structures.
  Chem Rev 118(8): 4156-4176

2017

• Wilkinson, M.E., Fica, S.M., Galej, W.P., Norman, C.M., Newman, A.J., Nagai, K. (2017)
  Postcatalytic spliceosome structure reveals mechanism of 3'-splice site selection.
  Science 358(6368): 1283-1288
• Scheres, S.H., Nagai, K. (2017)
  CryoEM structures of spliceosomal complexes reveal the molecular mechanism of pre-mRNA splicing.
  Curr Opin Struct Biol 46: 130-139
• Fica, S.M., Nagai, K. (2017)
  Cryo-electron microscopy snapshots of the spliceosome: structural insights into a dynamic ribonucleoprotein machine.
  Nat Struct Mol Biol 24(10): 791-799
• Plaschka, C., Lin, P.C., Nagai, K. (2017)
  Structure of a pre-catalytic spliceosome.
  Nature 546(7660): 617-621
• van Roon, A.M., Oubridge, C., Obayashi, E., Sposito, B., Newman, A.J., Séraphin, B., Nagai, K. (2017)
  Crystal structure of U2 snRNP SF3b components: Hsh49p in complex with Cus1p-binding domain.
  RNA 23(6): 968-981
• Fica, S.M., Oubridge, C., Galej, W.P., Wilkinson, M.E., Bai, X.C., Newman, A.J., Nagai, K. (2017)
  Structure of a spliceosome remodelled for exon ligation.
  Nature 542(7641): 377-380

2016

• Galej, W.P., Wilkinson, M.E., Fica, S.M., Oubridge, C., Newman, A.J., Nagai, K. (2016)
  Cryo-EM structure of the spliceosome immediately after branching.
  Nature 537(7619): 197-201
• Nguyen, T.H., Galej, W.P., Fica, S.M., Lin, P.C., Newman, A.J., Nagai, K. (2016)
  CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast.
  Curr Opin Struct Biol 36: 48-57
• Nguyen, T.H.D., Galej, W.P., Bai, X.C., Oubridge, C., Newman, A.J., Scheres, S.H.W., Nagai, K. (2016)
  Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution.
  Nature 530(7590): 298-302

2015

• Hardin, J.W., Warnasooriya, C., Kondo, Y., Nagai, K., Rueda, D. (2015)
  Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET.
  Nucleic Acids Res 43(22): 10963-74
• Nguyen, T.H., Galej, W.P., Bai, X.C., Savva, C.G., Newman, A.J., Scheres, S.H., Nagai, K. (2015)
  The architecture of the spliceosomal U4/U6.U5 tri-snRNP.
  Nature 523(7558): 47-52
• van Roon, A.M., Yang, J.C., Mathieu, D., Bermel, W., Nagai, K., Neuhaus, D. (2015)
  ¹¹³Cd NMR experiments reveal an unusual metal cluster in the solution structure of the yeast splicing protein Bud31p.
  Angew Chem Int Ed Engl 54(16): 4861-4
• van Roon, A.M., Yang, J.C., Mathieu, D., Bermel, W., Nagai, K., Neuhaus, D. (2015)
  Cd NMR Experiments Reveal an Unusual Metal Cluster in the Solution Structure of the Yeast Splicing Protein Bud31p.
  Angew Chem Weinheim Bergstr Ger 127(16): 4943-4946
• Nagai, K. (2015)
  In the beginning was the U1A protein: a personal reflection.
  RNA 21(4): 699-700
• Kondo, Y., Oubridge, C., van Roon, A.M., Nagai, K. (2015)
  Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition.
  Elife 4

2014

• Galej, W.P., Nguyen, T.H., Newman, A.J., Nagai, K. (2014)
  Structural studies of the spliceosome: zooming into the heart of the machine.
  Curr Opin Struct Biol 25(100): 57-66

2013

• Nguyen, T.H., Li, J., Galej, W.P., Oshikane, H., Newman, A.J., Nagai, K. (2013)
  Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.
  Structure 21(6): 910-19
• Galej, W.P., Oubridge, C., Newman, A.J., Nagai, K. (2013)
  Crystal structure of Prp8 reveals active site cavity of the spliceosome.
  Nature 493(7434): 638-43

2011

• Leung, A.K., Nagai, K., Li, J. (2011)
  Structure of the spliceosomal U4 snRNP core domain and its implication for snRNP biogenesis.
  Nature 473(7348): 536-9

2010

• Leung, A.K., Kambach, C., Kondo, Y., Kampmann, M., Jinek, M., Nagai, K. (2010)
  Use of RNA tertiary interaction modules for the crystallisation of the spliceosomal snRNP core domain.
  J Mol Biol 402(1): 154-64
• Janda, C.Y., Li, J., Oubridge, C., Hernández, H., Robinson, C.V., Nagai, K. (2010)
  Recognition of a signal peptide by the signal recognition particle.
  Nature 465(7297): 507-10
• Newman, A.J., Nagai, K. (2010)
  Structural studies of the spliceosome: blind men and an elephant.
  Curr Opin Struct Biol 20(1): 82-9

2009

• Oubridge, C., Krummel, D.A., Leung, A.K., Li, J., Nagai, K. (2009)
  Interpreting a low resolution map of human U1 snRNP using anomalous scatterers.
  Structure 17(7): 930-8
• Pomeranz Krummel, D.A., Oubridge, C., Leung, A.K., Li, J., Nagai, K. (2009)
  Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution.
  Nature 458(7237): 475-80

2008

• van Roon, A.M., et al. (2008)
  Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.
  Proc Natl Acad Sci U S A 105(28): 9621-6

2007

• Menichelli, E., Isel, C., Oubridge, C., Nagai, K. (2007)
  Protein-induced conformational changes of RNA during the assembly of human signal recognition particle.
  J Mol Biol 367(1): 187-203
• Obayashi, E., Oubridge, C., Pomeranz Krummel, D., Nagai, K. (2007)
  Crystallization of RNA-protein complexes.
  Methods Mol Biol 363: 259-76

2006

• Thompson, C., Pomeranz Krummel, D.A., Jacobsen, H., Nagai, K., Cooke, A. (2006)
  Identification of nuclear spliceosomal antigens targeted by NOD mouse antibodies following sodium iodide intake.
  Autoimmunity 39(2): 99-106

2005

• Ignjatovic, T., Yang, J.C., Butler, J., Neuhaus, D., Nagai, K. (2005)
  Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase.
  J Mol Biol 351(1): 52-65

2004

• Muto, Y., Pomeranz Krummel, D., Oubridge, C., Hernandez, H., Robinson, C.V., Neuhaus, D., Nagai, K. (2004)
  The structure and biochemical properties of the human spliceosomal protein U1C.
  J Mol Biol 341(1): 185-98

2003

• Nagai, K., Oubridge, C., Kuglstatter, A., Menichelli, E., Isel, C., Jovine, L. (2003)
  Structure, function and evolution of the signal recognition particle.
  EMBO J 22(14): 3479-85

2002

• Kuglstatter, A., Oubridge, C., Nagai, K. (2002)
  Induced structural changes of 7SL RNA during the assembly of human signal recognition particle.
  Nat Struct Biol 9(10): 740-4
• Oubridge, C., Kuglstatter, A., Jovine, L., Nagai, K. (2002)
  Crystal structure of SRP19 in complex with the S domain of SRP RNA and its implication for the assembly of the signal recognition particle.
  Mol Cell 9(6): 1251-61

2001

• Nagai, K., Muto, Y., Pomeranz Krummel, D.A., Kambach, C., Ignjatovic, T., Walke, S., Kuglstatter, A. (2001)
  Structure and assembly of the spliceosomal snRNPs. Novartis Medal Lecture.
  Biochem Soc Trans 29(Pt 2): 15-26
• Walke, S., Bragado-Nilsson, E., Séraphin, B., Nagai, K. (2001)
  Stoichiometry of the Sm proteins in yeast spliceosomal snRNPs supports the heptamer ring model of the core domain.
  J Mol Biol 308(1): 49-58
• Muto, Y., Krummel, D.A., Kambach, C., Hernandez, H., Robinson, C.V., Nagai, K. (2001)
  Reconstitution of the spliceosomal U1 snRNP from all recombinant subunits and its characterisation by ionspray Q-tof mass-spectrometry.
  Nucleic Acids Res Suppl (1): 275-6

2000

• Jovine, L., Hainzl, T., Oubridge, C., Nagai, K. (2000)
  Crystallization and preliminary X-ray analysis of the conserved domain IV of escherichia coli 4.5S RNA. erratum.
  Acta Crystallogr D Biol Crystallogr 56(# (Pt 11)): 1512
• Jovine, L., Hainz, T., Oubridge, C., Nagai, K. (2000)
  Crystallization and preliminary X-ray analysis of the conserved domain IV of Escherichia coli 4.5S RNA.
  Acta Crystallogr D Biol Crystallogr 56(Pt 8): 1033-7
• Jovine, L., et al. (2000)
  Crystal structure of the ffh and EF-G binding sites in the conserved domain IV of Escherichia coli 4.5S RNA.
  Structure 8(5): 527-40
• Muto, Y., Oubridge, C., Nagai, K. (2000)
  RNA-binding proteins: TRAPping RNA bases.
  Curr Biol 10(1): R19-21

1999

• Kambach, C., Walke, S., Nagai, K. (1999)
  Structure and assembly of the spliceosomal small nuclear ribonucleoprotein particles.
  Curr Opin Struct Biol 9(2): 222-30
• Kambach, C., et al. (1999)
  Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs.
  Cell 96(3): 375-87

1998

• Price, S.R., Evans, P.R., Nagai, K. (1998)
  Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA.
  Nature 394(6694): 645-50
• Varani, G., Nagai, K. (1998)
  RNA recognition by RNP proteins during RNA processing.
  Annu Rev Biophys Biomol Struct 27: 407-45

1996

• Price, S., Nagai, K. (1996)
  Secrets of RNA folding revealed.
  Structure 4(10): 1129-32
• Komiyama, N., Tame, J., Nagai, K. (1996)
  A hemoglobin-based blood substitute: transplanting a novel allosteric effect of crocodile Hb.
  Biol Chem 377(9): 543-8
• Jovine, L., Oubridge, C., Avis, J.M., Nagai, K. (1996)
  Two structurally different RNA molecules are bound by the spliceosomal protein U1A using the same recognition strategy.
  Structure 4(5): 621-31
• Allain, F.H., Gubser, C.C., Howe, P.W., Nagai, K., Neuhaus, D., Varani, G. (1996)
  Specificity of ribonucleoprotein interaction determined by RNA folding during complex formulation.
  Nature 380(6575): 646-50
• Avis, J.M., Allain, F.H., Howe, P.W., Varani, G., Nagai, K., Neuhaus, D. (1996)
  Solution structure of the N-terminal RNP domain of U1A protein: the role of C-terminal residues in structure stability and RNA binding.
  J Mol Biol 257(2): 398-411
• Nagai, K. (1996)
  RNA-protein complexes.
  Curr Opin Struct Biol 6(1): 53-61

1995

• Price, S.R., Nagai, K. (1995)
  Protein engineering as a tool for crystallography.
  Curr Opin Biotechnol 6(4): 425-30
• Price, S.R., Ito, N., Oubridge, C., Avis, J.M., Nagai, K. (1995)
  Crystallization of RNA-protein complexes. I. Methods for the large-scale preparation of RNA suitable for crystallographic studies.
  J Mol Biol 249(2): 398-408
• Oubridge, C., Ito, N., Teo, C.H., Fearnley, I., Nagai, K. (1995)
  Crystallisation of RNA-protein complexes. II. The application of protein engineering for crystallisation of the U1A protein-RNA complex.
  J Mol Biol 249(2): 409-23
• Nagai, K., Oubridge, C., Ito, N., Avis, J., Evans, P. (1995)
  The RNP domain: a sequence-specific RNA-binding domain involved in processing and transport of RNA.
  Trends Biochem Sci 20(6): 235-40
• Komiyama, N.H., Miyazaki, G., Tame, J., Nagai, K. (1995)
  Transplanting a unique allosteric effect from crocodile into human haemoglobin.
  Nature 373(6511): 244-6
• Nagai, K., Oubridge, C., Ito, N., Jessen, T.H., Avis, J., Evans, P. (1995)
  Crystal structure of the U1A spliceosomal protein complexed with its cognate RNA hairpin.
  Nucleic Acids Symp Ser (34): 1-2

1994

• Oubridge, C., Ito, N., Evans, P.R., Teo, C.H., Nagai, K. (1994)
  Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin.
  Nature 372(6505): 432-8
• Howe, P.W., Nagai, K., Neuhaus, D., Varani, G. (1994)
  NMR studies of U1 snRNA recognition by the N-terminal RNP domain of the human U1A protein.
  EMBO J 13(16): 3873-81

1993

• Evans, P.R., Oubridge, C., Jessen, T.H., Li, J., Teo, C.H., Pritchard, C., Ito, N., Nagai, K. (1993)
  Model of the interaction between the UI A small nuclear ribonucleoprotein and UI small nuclear RNA stem/loop II.
  Biochem Soc Trans 21 ( Pt 3)(3): 605-9
• Nagai, K. (1993)
  Recent advances in RNA-protein interaction studies.
  Mol Biol Rep 18(2): 105-12

1991

• Jessen, T.H., Oubridge, C., Teo, C.H., Pritchard, C., Nagai, K. (1991)
  Identification of molecular contacts between the U1 A small nuclear ribonucleoprotein and U1 RNA.
  EMBO J 10(11): 3447-56
• Komiyama, N.H., Shih, D.T., Looker, D., Tame, J., Nagai, K. (1991)
  Was the loss of the D helix in alpha globin a functionally neutral mutation?
  Nature 352(6333): 349-51

1990

• Nagai, K., Oubridge, C., Jessen, T.H., Li, J., Evans, P.R. (1990)
  Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A.
  Nature 348(6301): 515-20
• Neuhaus, D., Nakaseko, Y., Nagai, K., Klug, A. (1990)
  Sequence-specific [1H]NMR resonance assignments and secondary structure identification for 1- and 2-zinc finger constructs from SW15. A hydrophobic core involving four invariant residues.
  FEBS Lett 262(2): 179-84

1988

• Nagai, K., Nakaseko, Y., Nasmyth, K., Rhodes, D. (1988)
  Zinc-finger motifs expressed in E. coli and folded in vitro direct specific binding to DNA.
  Nature 332(6161): 284-6

1987

• Luisi, B.F., Nagai, K., Perutz, M.F. (1987)
  X-ray crystallographic and functional studies of human haemoglobin mutants produced in Escherichia coli.
  Acta Haematol 78(2-3): 85-9