Making a Chamber with Tape

This method can be used when imaging relatively thick samples (e.g. Daphnia or Volvox), which will probably be damaged when mounted directly between a coverslip and a slide.

  1. Place one to three layers of electrician's tape on a microscope slide (the number of layers depends on the thickness of the sample you are imaging).
  2. Using a Stanley knife, razor blade or scalpel, carefully score an approximately 1 cm by 1 cm square in the layers of tape.
  3. Peel away the central square of tape to make the chamber.
  4. Add the sample in a drop or two of liquid.
  5. Gently place a coverslip on the sample.
  6. Wick away any excess liquid by inserting tissue paper next to the cover slip.

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