Making a Chamber with Tape
This method can be used when imaging relatively thick samples (e.g. Daphnia or Volvox), which will probably be damaged when mounted directly between a coverslip and a slide.
- Place one to three layers of electrician's tape on a microscope slide (the number of layers depends on the thickness of the sample you are imaging).
- Using a Stanley knife, razor blade or scalpel, carefully score an approximately 1 cm by 1 cm square in the layers of tape.
- Peel away the central square of tape to make the chamber.
- Add the sample in a drop or two of liquid.
- Gently place a coverslip on the sample.
- Wick away any excess liquid by inserting tissue paper next to the cover slip.