(153) Establishing a synthetic orthogonal replication system enables accelerated evolution in E.coli.
Science. 2024. 383: 421-426.
R. Tian, F. B. H. Rehm, D. Czernecki, Y. Gu, J. F. Zurcher, K. C. Liu, J. W. Chin.
(152) Adding α,α-disubstituted & β-linked monomers to the genetic code of an organism.
Nature. 2024. 625: 603-610.
D. L. Dunkelmann, C. Piedrafita, A. Dickson, K. C. Liu, T. S. Elliott, M. Fiedler, D. Bellini, A. Zhou, D. Cervettini, J. W. Chin.
(151) Continuous synthesis of E. coli genome sections and megabase-scale human DNA assembly.
Nature 2023. 619: 555-562.
J. F. Zürcher, A. A. Kleefeldt, L. F. H. Funke, J. Birnbaum, J. Fredens, S. Grazioli, K. C. Liu, M. Spinck, G. Petris, P. Murat, F. B. H. Rehm, J. E. Sale, J. W. Chin.
(150) Quintuply orthogonal pyrrolysyl-tRNA synthetase/tRNAPyl pairs.
Nature Chem. 2023. 15: 948-959..120: e2301330120.
A. T. Beattie, D. Dunkelmann, J. W. Chin.
(149) Astrocytic control of extracellular GABA drives circadian timekeeping in the suprachiasmatic nucleus.
PNAS. 2023.120: e2301330120.
A. P. Patton, E. L. Morris, D. McManus, H. Wang, Y. Li, J. W. Chin, M. H. Hastings.
(148) Cell-of-origin-specific proteomics of extracellular vesicles.
PNAS Nexus 2023. 2: pgad107.
S. Kehrloesser, O. Cast, T. S. Elliott, R. J Ernst, A. C. Machel, J.-X. Chen, J. W. Chin, M. L. Miller.
(147) Genetically programmed cell-based synthesis of non-natural peptide and depsipeptide macrocycles.
Nature Chem. 2023 15: 61-69.
M. Spinck, C. Piedrafita, W. E. Robertson, T. S. Elliott, D. Cervettini, D. de la Torre, J. W. Chin.
(146) Refactored genetic codes enable bidirectional genetic isolation.
Science 2022 378: 516-523.
J. F. Zurcher, W. E. Robertson, T. Kappes, G. Petris, T. S. Elliott, G. P. C. Salmond, J. W. Chin.
(145) Bacterial divisome protein FtsA forms curved antiparallel double filaments when binding to FtsN.
Nature Microbiol. 2022 7: 1686-1701.
T.Nierhaus, S. H. McLaughlin, F. Bürmann, D. Kureisaite-Ciziene, S. L. Maslen, J. M. Skehel, C. W. H. Yu, S. Freund, L. F. H. Funke, J. W. Chin, Jan Löwe.
(144) Cryptochrome 1 as a state variable of the circadian clockwork of the suprachiasmatic nucleus: Evidence from translational switching.
Proc. Natl.Acad. Sci. 2022 119: e2203563119.
D. McManus, L. Polidarova, N. J. Smyllie, A. P Patton, J. E. Chesham, E. S. Maywood, J. W. Chin, M. H. Hastings.
(143) Discovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET.
Angew Chemie Int. Ed. 2022 e202203061.
B. Eiamthong, P. Meesawat,T. Wongsatit, J. Jitdee, R.Sangsri,M. Patchsung, K. Aphicho, S. Suraritdechachai, N. Huguenin-Dezot, S. Tang, W.Suginta, B. Paosawatyanyong, M. M. Babu, J. W. Chin, D. Pakotiprapha, W.Bhanthumnavin, C. Uttamapinant.
(142) RBBP6 activates the pre-mRNA 3’ end processing machinery in human cells.
Genes & Dev. 2022 36: 210-224.
V. Boreikaite, T. S. Elliott, J. W. Chin, L. A. Passmore.
(141) Mechansim-based traps enable protease and hydrolase substrate discovery.
Nature 2022 602: 701-707.
S. Tang, A. T. Beattie, L. Kafkova, G. Petris, N. Huguenin-Dezot, M. Fiedler, M. Freeman, J. W. Chin.
(140) Cryptochrome proteins regulate the circadian intra-cellular behaviour and localization of PER2 in mouse surachiasmatic nucleus neurons.
Proc. Natl. Acad. Sci. 2022 e2113845119.
N. J. Smyllie, J. Bagnall, A. A. Koch, D. Niranjan, L. Polidarova, J. E. Chesham, J. W. Chin, C. L. Partch, A. S. I. Loudon, M. H. Hastings.
(139) Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
Mol. Cell. 2021 81: 4891-4906.
F. Burman, L. F. H. Funke, J. W. Chin, J. Lowe.
(138) Precise optical control of gene expression in C. elegans using improved genetic code expansion and Cre recombinase.
eLife 2021 10:e67075.
L. Davis, I. Radmann, A. Goutou, A. Tynan, K. Baxter, Z. Xi, J. M. O’Shea, J. W. Chin, S. Greiss.
(137) A 68-codon genetic code to incorporate four distinct non-canonical amino acids enabled by automated orthogonal mRNA design.
Nature Chem. 2021 13: 1110-1117.
D. L. Dunkelmann, S. B. Oehm, A. T. Beattie, J. W. Chin.
(136) Sense codon reassignment enables viral resistance and encoded polymer synthesis.
Science 2021 372: 1057-1062.
W. E. Robertson, L. F. H. Funke, D. de la Torre, J. Fredens, T. S. Elliott, M. Spinck, Y. Christova, D. Cervettini, F. L. Boge, K. C. Liu, S. Buse, S. Maslen, G. P. Salmond, J. W. Chin.
(135) Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS.
Nature Protocols 2021 16: 2345-2380.
W. E. Robertson, L. F. H. Funke, D. de la Torre, J. Fredens, K. Wang, J. W. Chin.
(134) Selective CRAF inhibition elicits transactivation.
J. Am. Chem. Soc 2021 143: 4600-4606.
C. W. Morgan, I. L. Dale, A. P. Thomas, J. Hunt, J. W. Chin.
(133) High-efficacy subcellular micropatterning of proteins using fibrinogen anchors.
Cell. Biol 2021 220 (2): e202009063.
J. L. Watson, S. Aich, B. Oller-Salvia, A. A. Drakek, S. C. Blacklow, J. Chin, E. Derivery. J.
(132) Reprogramming the genetic code.
Nature Reviews Genetics 2021 22: 169-184.
D. de la Torre, J. W. Chin.
(131) Engineered triply orthogonal pyrrolysyl–tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids.
Nature Chem 2020 12: 535-544.
D.L. Dunkelmann, J. C. W. Willis, A. T. Beattie, J.W. Chin.
(130) Rapid discovery and evolution of orthogonal aminoacyl-tRNA synthetase/tRNA pairs.
Nature Biotech 2020 38: 989-999.
D. Cervettini, S. Tang, S. D. Fried, J. C. W. Willis, L. F. Funke, L. J. Colwell, J.W. Chin.
(129) Alcohol-derived DNA crosslinks are repaired by two distinct mechanisms.
Nature 2020 579:603-608.
M. R Hodskinson, A. Bolner, K. Sato, A. N. Kamimae-Lanning, K. Rooijers, M. Mohan, J. Silhan, M. Petek, D. M. Williams, J. Kind, J. W. Chin, K. J. Patel, P. Knipscheer.
(128) Programmed chromosome fission and fusion enable precise large-scale genome rearrangements and assembly.
Science 2019 365: 922-926.
K. Wang, D. de la Torre, W. E. Robertson, J.W. Chin.
(127) Efficient Phage display with multiple distinct non-canonical amino acids using orthogonal ribosome mediated genetic code expansion.
Angew. Chem. 2019 58: 10844-10848.
B. Oller-Salvia, J. W. Chin.
(126) Total synthesis of Escherichia coli with a recoded genome.
Nature. 2019 569: 514-518.
J. Fredens, K. Wang, D. de la Torre, L. F. H. Funke, W. E. Robertson, Y. Christova, T. Chia, W. H. Schmied, D. L. Dunkelmann, V. Beranek, C. Uttamapinant, A. Gonzalez Llamazares, T. S. Elliott, J. W. Chin.
(125) Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid.
Nature. 2019 565:112-117
N. Huguenin-Dezot, D. A. Alonzo, G. W. Heberlig, M. Mahesh, D. P. Nguyen, M. H. Dornan, C. N. Boddy, T. M. Schmeing, J. W. Chin.
(124) Controlling orthogonal ribosome subunit interactions enables evolution of new function.
Nature. 2018 564:444-448
W. H. Schmied, Z. Tnimov, C. Uttamapinant, C. D. Rae, S. D. Fried, J.W. Chin.
(123) Translational switching of Cry1 protein expression confers reversible control of circadian behavior in arrythmic Cry-deficient mice.
Proc. Natl. Acad. Sci. 2018 26:115
E. Maywood, T. Elliott, A. Patton, T. Krogager, J. Chesham, R. Ernst, V. Beranek, J. W. Chin, M. H. Hastings.
(122) An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA pair is Highly Active and Orthogonal in Mammalian Cells.
Biochemistry. 2018 58:387–390
V. Beranek, J.C.W.Willis, J. W. Chin.
(121) A conformational sensor based on genetic code expansion reveals an autocatalytic component in EGFR activation.
Nature Comms. 2018 9:3874.
M. Baumdick, M. Gelleri, C. Uttamapinant, V . Beranek, J. W. Chin, P. I. H. Bastiaens.
(120) Mitotic phosphorylation regulates the spindle localization of the chaperone Hsp72 by uncoupling ATP binding from substrate release.
Science Signalling. 2018 11:eaao2464.
M. Mukherjee, S. Sabir, L. O. Regan, J. Sampson, M. W. Richards, N. Huguenin-Dezot, J. R. Ault, J. W. Chin, A. Zhuraieva, A. M. Fry, R. Bayliss.
(119) Pyrophosphorylation via selective phosphoprotein derivatization.
Chem. Sci. 2018 9:5929-5936
A. M. Marmelstein, J. A. M. Morgan, M. Penkert, D. T. Rogerson, J. W. Chin, E. Krause, D. Fiedler.
(118) Genetically encoded protein phosphorylation in mammalian cells.
Cell Chem. Biol. 2018 25:1067-1074
V. Beranek, C. D.Reinkemeier, M. S. Zhang, A. D. Liang, G. Kym, J.W. Chin.
(117) Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs.
Nature Chem. 2018 10:831-837
J.C.W. Willis, J.W. Chin.
(116) Intracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21.
eLIFE. 2018 7:e32660
C. Dickson, A. Fletcher, M. Vaysburd, J.-C. Yang, D.L. Mallery, J. Zeng, C.M. Johnson, S.H. Mclaughlin, M. Skehel, S. Maslen, J. Cruikshank, N. Huguenin-Dezot, J. W. Chin, D. Neuhaus, L.C. James
(115) Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic α-helix.
EMBO. J. 2018 37:e97902
S. G. Burgess, M. Murkherjee, S. Sabir, N. Joseph, C. Gutiérrez-Caballero, M.W. Richards, N. Huguenin-Dezot, J.W. Chin, E.J. Kennedy, M. Pfuhl, S.J. Royle, F. Gergely, R. Bayliss.
(114) Detecting RNA base methylations in single cells by in situ hybridization.
Nature Comms. 2018 9:655
R. T. Ranasinghe, M. R. Challand, K. A. Ganzinger, B. W. Lewis, C. Softley, W. H. Schmied, M. H. Horrocks, N. Shivji, J. W. Chin, J. Spencer, D. Klenerman.
(113) Rapid and efficient generation of stable antibody drug conjugates via an encoded cyclopropene and an inverse electron demand Diels Alder reaction.
Angew. Chem. 2018 57:2831–2834.
B. Oller-Salvia, G.Kym, J.W. Chin.
(112) An Orthogonal Central Dogma.
Nature Chem Biol. 2018 16;14:103-106
C. Liu, M. Jewett, J.W. Chin, C. A. Voigt.
(111) Labelling and identifying cell-type-specific proteomes in the mouse brain.
Nature Biotech. 2018 36:156-159.
T. P. Krogager, R. J. Ernst, T. S. Elliott, L. Calo, V. Beranek, E. Ciabatti, M. Grazia Spilantini, M. Tripodi, M. H. Hastings, J. W. Chin.
(110) Encoding Optical control in LCK kinase to quantitatively investigate its activity in live cells.
Nature Struct. Mol. Biol. 2017 24:1155-1163
A. Liaunardy-Jopeace, B.L. Murton, M. Mahesh, J.W. Chin, J.R. James.
(109) Expanding and Reprogramming the Genetic Code.
Nature. 2017 550:53-60
J. W. Chin.
(108) Computationally guided discovery of reactive, hydrophilic trans-5-oxocene dienophile for bioorthogonal labeling.
Org. Biomol. Chem. 2017 15:6640-6644
W.D Lamber, S. L. Scinto, O. Dmiternko, S.J. Boyd, R. Magboo, R. A. Mehl, J. W. Chin, J.M. Fox, S. Wallace.
(107) Intein Zymogens: Conditional Assembly and Splicing of Split Inteins via Targeted Proteolysis
J. Am. Chem. Soc. 2017 5139:8074-8077
J.A. Gramespacher, A.J. Stevens, D.P. Nguyen, J.W. Chin, T.W. Muir.
(106) Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing. Nature Meths. 2017 14: 729-736
M.S Zhang, S.F. Brunner, N. Huguenin-Dezot, A.D. Liang, W.H. Schmied, D.T. Rogerson, J.W. Chin.
(105) Protein modification via alkyne hydrosilylation using a sub-stoichiometric amount of ruthenium (II) catalyst
Chem. Sci. 2017
T.T.-L. Kwan, O. Boutureira, E. C. Frye, S. J. Walsh, M. K. Gupta et al.
(104) Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET.
Sci Rep. 2017 7:39841
L. Perdios, A.R. Lowe, G. Saladino, T.D. Bunney, N. Thiyagarajan, Y. Alexandrov, C. Dunsby, P.M. French, J.W Chin, F.L. Gervasio, E.W. Tate, M. Katan.
(103) Defining Synonymous Codon Compression Schemes by Genome Recoding.
Nature 2016 539:59-64
K. Wang, J. Fredens, S.F. Brunner, S.H. Kim, T. Chia, J. W. Chin.
(102) Genetic code expansion in the mouse brain.
Nature Chem. Biol. 2016 12: 776-778
R. J. Ernst, T. P. Krogager, E. S. Maywood, R. Zanchi, V. Beranek, T. S. Elliott, N. P. Barry, M. H. Hastings, J. W. Chin.
(101) Tagging and enriching proteins enables cell specific proteomics.
Cell Chem. Biol. 2016 23: 805-815
T. S. Elliott, A. Bianco, F. M. Townsley, S. D. Fried, J. W. Chin.
(100) Synthesis of isomeric phosphoubiquitin chains reveals that phosphorylation controls deubiquitinase activity and specificity.
Cell Rep. 2016 16: 1180-1193
N. Huguenin-Dezot, V. De Cesare, J. Peltier, A.Kneble, Y. Adi Kristaryanto, D. T. Rogerson, Y. Kulathu, M. Trost, J. W. Chin
(99) Genetically encoded optical activation of DNA recombination in human cells.
Chem Comm. 2016 52: 8529-8532
J. Luo, E. Arbely, J. Zhang, C. Chou, R. Uprety, J. W. Chin, A. Deiters.
(98) Photoactivation of mutant isocitrate dehydrogenase 2 reveals rapid cancer-associated metabolic and epigenetic changes.
J. Am. Chem. Soc. 2016 138: 718-721
O.S. Walker, S. J. Elsaesser, M. Mahesh, M. Bachman, S. Balasubramanian, J.W. Chin.
(97) Genetic code expansion in stable cell lines enables encoded chromatin modification.
Nature Meths. 2016 13: 158-164
S J. Elsaesser, R. J. Ernst, O. S. Walker, J. W. Chin.
(96) Structural and mechanistic insight into the regulation of fundamental Rho-regulator RhoGDIα by lysine acetylation.
J. Biol. Chem. 2015 doi: 10.1074/jbc.M115.707091
N. Kuhlman, S. Wroblowski, P. Knyphausen, S. deBoor, J. Brenig, A. Y. Zienert, K. Meyer-Teschendorf, G.J.K Praefcke, H. Nolte, M. Krueger, M. Schacherl, U. Baumann, L. C. James, J.W. Chin, M. Lammers.
(95) EGF-dependent re-routing of vesicular recycling switches spontaneous phosphorylation suppression to EGFR signaling.
eLIFE 2015 doi: 10.7554/eLife.12223
M. Baumdick, G. Xouri, Y. Bruggemann, L. Davis, M. Schmick, O. Sabet, J. W. Chin, P. Bastiaens.
(94) Ribosome subunit stapling for orthogonal translation in E. coli.
Angew. Chem. 2015 54: 12791-12994.
S. Fried, W. H. Schmied, C. Uttamapiniant, J. W. Chin.
(93) Efficient genetic encoding of phosphoserine and its non-hydrolyzable analog.
Nature Chem Biol. 2015 11: 496-503
D. T. Rogerson, A. Sachdeva, K. Wang, T. Haq, N. Huguenin-Dezot, M. M. Muqit, A. Fry, R. Bayliss, J.W. Chin.
[Highlighted, Nature Methods 2015 12: 702-703.]
(92) Selective rapid and optically switchable regulation of protein function in live mammalian cells.
Nature Chem. 2015 7: 554-561
Y-H. Tsai, S. Essig, J. R. James, K. Lang, J. W. Chin.
[Highlighted, Nature Methods 2015 12: 601. ACS Chem. Biol. 2015 10: 1355. Chem. Eng. News. May 22, 2015]
(91) Genetic code expansion enables live-cell super-resolution imaging of site-specifically labeled cellular protein.
J. Am. Chem. Soc. 2015 137: 4602-4605
C. Uttamapinant, J. D. Howe, K. Lang, V. Beranek, L. Davis, M. Mohan, N. P. Barry, J. W. Chin.
(90) Ubiqutination of the Dishevelled DIX domain blocks its head-to-tail polymerization.
Nature Comms. 2015 6: 6718
J. Madrzak, M. Fiedler, C. M. Johnson, R. Ewan, A. Knebel, M. Bienz, J. W. Chin.
(89) Site specific glycoconjugation of protein via a bioorthognal tetrazine cycloaddition with a genetically encoded trans-cyclooctene or bicyclononyne.
Bioconjug. Chem. 2015 26: 802-806.
T. Machida, K. Lang, L. Xue, J.W. Chin, N. Wissinger.
(88) Genetic encoding of unnatural amino acids for labeling proteins.
Methods Mol Biol. 2015 1266:217-228
K. Lang, L. Davis, J.W. Chin.
(87) Ubiquitin C-terminal hydrolyases cleave isopeptide and peptide-linked ubiquitin from structured proteins but do not edit ubiquitin homopolymers.
Biochem J. 2015 466:489-98
J. S. Bett, M. S. Ritorto, R. Ewan, E. G. Jaffray, S. Virdee, J. W. Chin, A. Knebel, T. Kurz, M. Trost, M. H. Tatham, R. Hay.
(86) Host cofactors and pharmagologic ligands share an essential interface in HIV-1 capsid that is lost upon disassembly.
PLoS Pathog. 2014 10 e1004459
A. J. Price, D. A. Jaques, W. A. McEwan, A. J. Fletcher, S. Essig, J. W. Chin, U. D. Hamblage, C. Aiken, L. C. James.
(85) Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl-tRNA synthetase/tRNA expression and engineered eRF1.
J. Am. Chem. Soc. 2014 136:15577-15583
W. Schmied, S. J. Elsaesser, C. Uttamapinant, J. W. Chin.
(84) Genetically encoded optochemical probes for simultaneous fluorescence reporting and light activation of protein function with two-photon excitation.
J. Am. Chem. Soc. 2014 136:15551-15558
J. Luo, R. Uprety, Y. Naro, C. Chou, D. P. Nguyen, J. W. Chin, A. Deiters.
(83) Genetic code expansion and bioorthogonal labeling enables cell specific proteomics in an animal.
Curr. Opin. Chem. Biol. 2014 21:154-160
T.S. Elliott, A. Bianco, J.W. Chin.
(82) Conformationally strained trans-cyclooctene with improved stability and excellent reactivity in tetrazine ligation.
Chem. Sci. 2014 5:3770-3776
A. Darko, S. Wallace, O. Dmitrenko, M. M. Machovina, R. A. Mehl, J. W. Chin, J. M. Fox. Chem. Sci. 2014 5: 3770-3776
(81) Concerted, Rapid, Quantitative, and Site-specifc Dual Labeling of Proteins
J. Am. Chem. Soc. 2014 136:7785–7788.
A. Sachdeva, K. Wang. T. Elliott, J.W. Chin.
(80) Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double labeling and FRET
Nature Chemistry. 2014 6:393-403.
K. Wang, A. Sachdeva, D. J. Cox, N. M. Wilf, K. Lang, S. Wallace, R. A. Mehl, J. W. Chin.
[Featured in News & Views Nature Chem. 2014 6:379-381]
(79) Proteome Labeling and Protein Identification in Specific Tissues at Specific Developmental Stages in an Animal
Nature Biotech.2014 32:465-472.
T.S. Elliott, F.M. Townsley, A. Bianco, R.J. Ernst, A. Sachdeva, S.J. Elsaesser, L. Davis, K. Lang, R. Pisa, S. Greiss, K.S. Lilley, J.W. Chin.
[Featured in News & Views, Nature Biotech. 2014 32:445-446, Spotlight, ACS Chem. Biol. 2014 9:1068]
(78) Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Chem. Rev. 2014 114:4764-4806.
K. Lang, J.W. Chin.
(77) Expanding and Reprogramming the Genetic Code of Cells and Animals
Ann. Rev. Biochem. 2014 83:379-408
J.W. Chin.
(76) Strain promoted sydnone bicyclo-[6.1.0]-nonyne cycloaddition
Chem Sci. 2014 5:1742-1744.
S. Wallace, J.W. Chin.
(75) Genetic Encoding of Photocaged Cysteine Allows Photoactivation of TEV Protease in Live Mammalian Cells.
J. Am. Chem. Soc. 2014 136:2240-2243.
D.P. Nguyen, M. Mahesh, S.J. Elsaesser, S. M. Hancock, C. Uttamapinant, J.W. Chin.
(74) Bioorthogonal reactions for labeling proteins
ACS Chem. Biol. 2014 9:16-20.
K. Lang, J.W. Chin.
(73) Genetically encoded light activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells
J. Am. Chem. Soc. 2013 135:13433-13439.
J. Hempill, C. Chou, J.W. Chin, A. Deiters.
(72) Efficient and Rapid C.elegans Transgenesis by Bombardment and Hygromycin B selection
PLOS ONE 2013 135:76019.
I. Radman, S. Greiss, J.W. Chin.
(71) Fluorescent imaging: Shining a light into live cells
Nature Chemistry. 2013 5:81-82.
K. Lang, J.W. Chin.
(70) DAXX envelops an H3.3–H4 dimer for H3.3-specific recognition.
Nature. 2012 491:560–565
S.J. Elsässer, H. Huang, P.W. Lewis, J.W. Chin, C.D. Allis, D.J. Patel
(69) Expanding the Genetic Code of Drosophila melanogaster.
Nature Chem. Biol. 2012 8:748-750.
A. Bianco, F.M. Townsley, S. Greiss, K. Lang, J.W. Chin.
(68) CPSF6 Defines a Conserved Capsid Interface that Modulates HIV-1 Replication.
PLoS Pathog. 2012 8(8): e1002896.
A. J. Price, A. J. Fletcher, T. Schaller, T. Elliott, K. Lee, V. N. Kewal Ramani, J. W. Chin, G. J. Towers, L. C. James.
(67) A Different Life?
Curr. Opin. Chem. Biol. 2012 16: 243-244
J.W. Chin, L. You.
(66) Photocontrol of Tyrosine Phosphorylation in Mammalian Cells via Genetic Encoding of Photocaged Tyrosine.
J. Am. Chem. Soc. 2012 134: 11912-11915.
E. Arbeley, J. Torres-Kolbus, A. Deiters, J.W. Chin.
(65) Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Rapid Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Fluorogenic Diels-Alder Reactions.
J. Am. Chem. Soc. 2012 134:10317-10320
K. Lang, L. Davis, S. Wallace, M. Mahesh, D. J. Cox, M. L. Blackman, J. M. Fox & J.W. Chin.
(64) Reprogramming the genetic code.
Science. 2012 336:428-429.
J.W. Chin.
(63) Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal cycloaddition.
Nature Chem. 2012 4:298-304.
K. Lang, L. Davis, A Deiters, J.W. Chin.
[Featured in Nature Chem. 2012 4: 248-250]
(62) Designer Proteins: Applications of Genetic Code Expansion in Cell Biology.
Nature Rev. Mol. Cell. Biol. 2012 13:168-182
L. Davis & J.W. Chin.
(61) Reprogramming the Genetic Code: From Triplet to Quadruplet Codes.
Angew Chem. 2012 51:2288-2297.
K. Wang, W. Schmeid & J.W. Chin.
(60) An Ankyrin-repeat ubiquitin-binding domain determines TRABID’s specificity for atypical ubiquitin chains.
Nature Struct. Mol. Biol. 2011 19:62-71
J.D.F. Licchesi, J. Miezczanek, T.E.T. Mevissen, T.J. Rutherford, M. Akutsu, S. Virdee, F.E. Oualid, J.W. Chin, H. Ovaa, M. Bienz, D. Komander.
(59*) Expanding the Genetic Code of an Animal.
J. Am. Chem. Soc. 2011 133:1496-14199
S. Greiss & J.W. Chin.
[Featured on the BBC (www.bbc.co.uk/news/science-environment-14492948), BBC World Service Radio (http://www.bbc.co.uk/programmes/p00jttd6), Chemistry World (http://www.rsc.org/chemistryworld/News/2011/August/17081102.asp), ChemBiochem. 2012 DOI: 10.1002/cbic.201100674]
(58*) Traceless & Site-Specific Ubiquitination of Recombinant Proteins.
J. Am. Chem. Soc. 2011 133:10708-10711
S. Virdee, P.B. Kapadnis, T. Elliott, K. Lang, J. Madrzak, D.P. Nguyen, L. Riechmann, & J.W. Chin.
(57) Genetically Encoded 1,2 Aminothiols Facilitate Rapid and Site Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation.
J. Am. Chem. Soc. 2011 133:11418-11421
D.P. Nguyen, T. Elliott, M. Holt, T.W. Muir, & J.W. Chin.
[Featured in ACS Chem Biol. 2011 8: 768-770]
(56*) Reprogramming the Genetic Code.
EMBO J. 2011 30: 2312-2324
J.W. Chin.
(55) Orthogonal Gene Expression in Escherichia coli.
Methods Enzymol. 2011 497: 115-1134
W. An, J.W. Chin.
(54) A Dual Role of H4K16 Acetylation in the Establishment of Yeast Silent Chromatin.
EMBO J. 2011 30:2610-2621
M. Oppikofer, S. Kueng, F. Martino, S. Soeroes, S.M. Hancock, J.W. Chin, W. Fischle, S.M. Gasser.
(53) Acetylation of Lysine 120 of p53 endows DNA-binding Specificity at Effective Physiological Salt Concentration.
Proc. Natl. Acad. Sci. U.S.A. 2011 108:8251-8256
E. Arbely, E. Natan, T. Brandt, M.D. Allen, D.B. Veprintsev, C.V. Robinson, J.W. Chin, A.C. Joerger, A.R. Fersht.
(52*) Light Activated Kinases Enable Temporal Dissection of Signaling Networks in Living Cells.
J. Am. Chem. Soc. 2011 133: 2125-2127
A. Gautier, A. Deiters, & J.W. Chin.
[Featured in Nature Meths. 2011 4:284-285, Chem. Eng. News 14 Feb. 2011]
(51) Molecular Basis for Ubiquitin and ISG15 Cross-reactivity in Viral Ovarian Tumor Domains.
Proc. Natl. Acad. Sci (USA) 2011 108: 2228-2233
M. Akutsu, Y. Ye, S. Virdee, J.W. Chin, D. Komander.
(50) Genetically Encoding an Aliphatic Diazirine for Protein Photocrosslinking.
Chem. Sci. 2011 2: 480-483
C. Chou, R. Uprety, L. Davis, J.W. Chin, A. Deiters.
(49*) Engineered Diubiquitin Synthesis Reveals K29 Isopeptide Specificity of an OTU Deubiquitinase.
Nature Chem. Biol. 2010 6:750-757
S. Virdee, Y. Ye, D.P. Nguyen, D. Komander & J. W. Chin.
[Featured in Chem. Eng. News. Oct 11 2010, Chem. Eng. News (Highlights of the Year) Dec 20 2011., Angew. Chem. 2011 49:9042-9044]
(48*) Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA synthetase/tRNA Pair.
J. Am. Chem. Soc. 2010 132:14819-14824
S. M. Hancock, R. Uprety, A. Deiters & J.W. Chin.
(47*) Genetically Directing Ne, Ne dimethyl-L-lysine in Recombinant Histones.
Chem. Biol. 2010 17:1072-1076
D.P. Nguyen, M. Garcia-Alai, S. Virdee & J.W. Chin.
(46*) Genetically Encoded Photo-control of Protein Localization in Mammalian Cells.
J. Am. Chem. Soc. 2010 132: 4086-4088
A Gautier, D.P. Nguyen, H. Lusic, W. An, A. Deiters, J.W. Chin.
[Featured in Chem. Bio Chem. 2010 11:1825-1827]
(45*) Encoding Multiple Unnatural Amino Acids via a Quadruplet Decoding Ribosome.
Nature. 2010 468:441-444.
H. Neumann, K. Wang, L. Davis, M. Garcia-Alai, & J.W. Chin.
[Featured in Nature Meths 2010 7: 343, New Scientist 17 Feb 2010, Chem. Eng. News 22 Feb 2010 v88: 8, p9, National Public Radio (USA) Morning Edition, 15 Feb 2010]
(44*) De Novo Generation of Mutually Orthogonal Aminoacyl-tRNA synthetase/tRNA pairs.
J. Am. Chem. Soc. 2010. 132:2142-2144
H. Neumann, A. Slusarczyk, J.W. Chin.
[Featured in New Scientist 17 Feb 2010. Chem. Eng. News 22 Feb 2010 88: 8, p9]
(43) Regulation of Cellular Metabolism by Protein Lysine Acetylation.
Science. 2010. 237:1000–1004
22 co-authors
[Perspective in Science 2010 327: 964-965]
(42*) Acetylation Regulates Cyclophilin A Catalysis, Immunosuppression & HIV Isomerization.
Nature Chem. Biol. 2010 6:331-337
M. Lammers, H. Neumann, J.W. Chin, L. C. James.
[Featured in Chem. Eng. News. 12 April 2010 88:15 p28]
(41) Evolved Orthogonal Ribosome Purification for in vitro Characterization.
Nucleic. Acids Res. 2010 38:2682-2691
O. P. T. Barrett & J. W. Chin.
(40*) Genetically Encoding Ne-methyl-L-lysine in Recombinant Histones.
J. Am. Chem. Soc. 2009. 131:14194-14195
D. P. Nguyen, M. Garcia Alai, P.B. Kapadnis, H. Neumann, & J.W. Chin.
(39*) A Method for Genetically Installing Site-specific Acetylation in Recombinant Histones Defines the Effects of H3 K56 Acetylation.
Mol. Cell. 2009. 36:153-163
H. Neumann, S. Hancock, R. Buning, A. Routh, L. Chapman, J. Sommers, T. Owen-Hughes, J. van Noort, D. Rhodes, & J.W. Chin.
[Featured in Nature Chem. Biol. 2009. 5: 787; Chem. Biol. 2009. 16:1027-1029. Nature Methods 2009 6: 865]
(38*) Genetic Encoding and Labeling of Aliphatic Azides and Alkynes in Recombinant Proteins via a Pyrrolysyl-tRNA Synthetase/tRNACUA Pair and Click Chemistry.
J. Am. Chem. Soc. 2009. 131: 8720-8721
D. P. Nguyen, H. Lusic, P.B. Kapadnis, A. Deiters & J.W. Chin.
(37*) Synthesis of Orthogonal Transcription-Translation Networks.
Proc. Natl. Acad. Sci. U.S.A. 2009. 106: 8477-8482.
W. An & J.W. Chin.
[Featured in Nature 2010 463:288-290]
(36) A Molecular Biologist Gets Excited About Making Designer Proteins in Cells.
Nature. 2009 457: 239
J.W. Chin.
(35*) Genetically Encoding Ne-acetyllysine in Recombinant Proteins.
Nature Chem. Biol. 2008. 4: 232-234.
H. Neumann, S.Y. Peak-Chew & J.W. Chin.
[Featured in Nature 2008. 45: 868, Chemistry & Engineering News (ACS). 2008. Feb 25, p38, Chemistry World (RSC), 2008. Feb 18]
(34) Genetically Encoding Protein Oxidative Damage.
J. Am. Chem. Soc. 2008. 130: 4028-33
H. Neumann, J.L. Hazen, J. Weinstein, R. A. Mehl & J. W. Chin.
(33) Engineering Escherichia coli Heat-Resistance by Synthetic Gene Amplification.
Prot. Eng. Des. Sel. 2008. 21: 121-125.
D.U. Christ & J.W. Chin.
(32) Current & Future Prospects in Biopolymer Chemistry.
Curr. Opin. Chem. Biol. 2007. 11:626-627
J.W. Chin, J.J. Kohler.
(31) Reprogramming the amino-acid substrate specificity of orthogonal aminoacyl-tRNA synthetases to expand the genetic code of eukaryotic cells.
Nature Prot. 2007. 2: 2590-2600.
T. Ashton Cropp, J. Christopher Anderson, J. W. Chin.
(30*) Evolved Orthogonal Ribosomes Enhance the Efficiency of Synthetic Genetic Code Expansion.
Nature Biotech. 2007. 25: 770-777.
K. Wang, H. Neumann, J. W. Chin.
[News & Views Nature Biotech. 2007. 25: 745-746.]
(29) Expanding Nucleic Acid Function in vitro and in vivo.
Curr. Opin. Chem. Biol. 2006. 10: 601-606.
T. A. Cropp, J. W. Chin.
(28) Programming and Engineering Biological Networks.
Curr. Opin. Struct. Biol. 2006. 16: 551-556.
J. W. Chin.
(27*) Modular Approaches to Expanding the Functions of Living Matter.
Nature Chem. Biol. 2006. 2: 304-311.
J. W. Chin.
(26*) Functional Epitopes at the Ribosome Subunit Interface.
Nature Chem. Biol. 2006. 2: 254-258.
O. Rackham, K. Wang & J. W. Chin.
[News & Views, Nature Chem. Biol. 2006. 2: 231-232]
(25) Synthesizing Cellular Networks from Evolved Ribosome•mRNA Pairs.
Biochem. Soc. Trans. 2006. 34: 328-329.
O. Rackham & J. W. Chin.
(24) A Genetically Encoded, Fluorescent Amino Acid.
Proc. Natl. Acad. Sci. U.S.A. 2006. 103: 9785-9789.
D. Summerer, S. Chen, N. Wu, A. Deiters, J. W. Chin, P. G. Schultz.
(23) Cellular Logic with Orthogonal Ribosomes.
J. Am. Chem. Soc. 2005. 127: 17584-17585.
O. Rackham & J. W. Chin.
[Featured in Science 2005. 310: 1587]
(22*) A Network of Orthogonal Ribosome•mRNA Pairs.
Nature Chem. Biol. 2005. 1: 159-166.
O. Rackham & J. W. Chin.
[Featured in Nature 2005. 436: 306-307; Science 2005. 310: 1587; Chemistry & Engineering News, 2005, July 25, p44; Nature Methods 2005. 2: 640]
(21) Photocrosslinking Proteins in vitro and in E .coli via the Genetic Incorporation of p-benzoyl-L-phenylalanine.
Nature Methods 2005. 2: 377-384.
I. S. Farrell, R. Toroney, J. L. Hazen, R. A. Mehl & J. W. Chin.
(20) Protein Expression by Expansion of the Genetic Code.
Encyclopedia of Molecular Cell Biology & Molecular Medicine (2nd edition, Ed. Myers, R. A.), Wiley-VCH 2005. 11: 45-68.
J. W. Chin, T. J. Magliery.
(19) Substrate Recognition by the AAA+ Chaperone ClpB.
Nature Struct. Mol. Biol. 2004. 11: 607-615.
C. Schlieker, J. Weibezahn, H. Patzelt, P. Tessarz, C. Strub, K. Zeth, A. Erbse, J. Schneider-Mergener, J. W. Chin, P. G. Schultz, B. Bukau, A. Mogk.
(18) Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces cerevisiae.
J. Am. Chem. Soc. 2003. 125: 11782-11783.
A. Deiters, T. A. Cropp, M. Mukherji, J. W. Chin, J. C. Anderson, P. G. Schultz.
(17) An Expanded Eukaryotic Genetic Code.
Science 2003. 301: 964-967.
J. W. Chin, T. A. Cropp, J. C. Anderson, M. Mukherji, Z. Zhang, and P. G. Schultz.
[Featured in Nature Biotech. 2003. 21: 1023; Science News 2003. 164: 102; The Scientist, August 15 2003; Chemistry & Engineering News, 2003, August 18, p49; Scientific American, 2004. 290: 74-81; Bioessays 2004. 26: 111-115]
(16) Progress Toward an Expanded Eukaryotic Genetic Code.
Chem. Biol. 2003. 10: 511-519.
J. W. Chin, T. A. Cropp, S. Chu, E. Meggers, and P. G. Schultz.
[Featured in Chem. Biol. 2003. 10: 475-476; Chem. Biol. 2003. 10: 586-587]
(15) In vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis.
ChemBioChem. 2002. 11: 1135-1137.
J. W. Chin and P. G. Schultz.
(14) Addition of a Photocrosslinking Amino Acid to the Genetic Code of Escherichia coli.
Proc. Natl. Acad. Sci. U.S.A. 2002. 99: 11020-11024.
J. W. Chin, A. B. Martin, D. S. King, L. Wang and P. G. Schultz.
[Featured in Trends Microbiol. 2002. 10: 552]
(13) Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli.
J. Am. Chem. Soc. 2002. 124: 9026-9027.
J. W. Chin, S. W. Santoro, A. B. Martin, D. S. King, L. Wang and P. G. Schultz.
(12) A View to a Kill: Ligands for Bcl-2 Family Proteins.
Curr. Opin. Chem. Biol. 2002. 6: 479-485.
S. E. Rutledge, J. W. Chin and A. Schepartz.
(11) Design and Evolution of a Miniature Bcl-2 Binding Protein.
Angew. Chem. Intl. Ed. Engl. 2001. 40: 3806-3809.
J. W. Chin and A. Schepartz.
(10) Concerted Evolution of Structure and Function in a Miniature Protein.
J. Am. Chem. Soc. 2001. 123: 2929-2930.
J. W. Chin and A. Schepartz.
[Featured in Science 2001. 291: 2049]
(9) Methodology for Optimizing Miniature Proteins based on Avian Pancreatic Polypeptide Using Phage Display.
Bioorg. Med. Chem. Lett. 2001. 11: 1501-1505.
J. W. Chin, R. M. Grotzfeld, M. A. Fabian and A. Schepartz.
(8) Eukaryotic Gene Regulation.
Chem. Biol. 2000. 6: R6.
J. W. Chin.
(7) Gene Regulation: Protein Escorts to the Transcription Ball.
Curr. Biol. 1999. 9: R929-932.
J. W. Chin, J. J. Kohler, T. L. Schneider and A. Schepartz.
(6) The Vault Reports Guide to Mastering Chemistry.
1998, Houghton Mifflin, New York.
J. Chin.
(5) Forest Idyll.
New Scientist 1996, 152, 2051, 42.
J. Chin.
(4) Resistance is Useless.
New Scientist 1996, 152, 2051, 42.
J. Chin.
(3) Fear and Loathing in the Midbrain.
New Scientist 1996, 152, 2051, 42.
J. Chin.
(2) An Interactive Night at the Opera.
New Scientist 1996, 152, 2051, 42.
J. Chin.
(1) Potassium Gives in Under Pressure.
New Scientist 1996, 152, 2051, 42.
J. Chin.