EpsinR: an AP1/clathrin interacting protein
involved in vesicle trafficking
Myc-tagged and endogenous epsinR versus epsinR-GFP
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EpsinR ENTH-GFP co-localises with AP1 at a TGN-like compartment. The N-terminally Myc-tagged protein is cytoplasmic. We do not know the reason for this discrepancy. but we see the same distribution for full-length epsinR whether it has an N-terminal Myc tag or a C-terminal GFP tag. |
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EpsinR ENTH-GFP - green |
myc-EpsinR ENTH - green |
EpsinR-GFP versus AP1 and clathrin
There is good overlap between the perinuclear epsinR-GFP and endogenous AP1 and clathrin
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| EpsinR GFP - green
large image (36 kb) |
AP1 - red
large image (76 kb) |
merge
large image (84 kb) |
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| EpsinR GFP - green
large image (40 kb) |
AP1 - red
large image (36 kb) |
merge
large image (56 kb) |
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| EpsinR GFP - green
large image (16 kb) |
Clathrin - red
large image (44 kb) |
merge
large image (40 kb) |
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| EpsinR GFP - green
large image (24 kb) |
Clathrin - red
large image (76 kb) |
merge
large image (44 kb) |
Comparison of epsin1 and epsinR
Myc-epsinR (WT) overexpression and colocalisation with markers
Myc-epsinR D34G+R67L (lipid binding mutant)
Myc-epsinR L10E (proposed lipid conformation mutant)
Myc-epsinR D342R+D422R (clathrin binding mutant)
Myc-epsinR D34G+R67L + D342R+D422R (lipid and clathrin binding mutant)
EpsinR-GFP and ENTH domain overexpression