EpsinR D34G + R67L overexpression in COS cells

This pair of mutations abolishes PtdInsP binding

Myc-EpsinR D34G + R67L with endogenous AP1 and internalised transferrin in COS cells

This mutant has a different distribution to WT epsinR, being found in more peripheral puncta. This shows that the ENTH domain is essential for efficient TGN targeting. The co-localisation images (see below) do not identify the labelled compartment, but there is a redistribution of perinuclear clathrin into these puncta and also a redistribution of TGN46 into these puncta. As seen on the left, AP1 and internalised transferrin do not co-localise.

Click image to view individual channels or proceed to galleries of images below.

Colocalisation of EpsinR D34G + R67L (green) with markers (red), focusing on the perinuclear region.

M6P receptor
Zoomed Image (81 kb)

Blue - Transferrin
Zoomed Image (90 kb)

EpsinR cover page

Endogenous epsinR expression

Comparison of epsin1 and epsinR

Myc-epsinR (WT) overexpression and colocalisation with markers

Myc-epsinR D34G+R67L (lipid binding mutant)

Myc-epsinR L10E (proposed lipid conformation mutant)

Myc-epsinR D342R+D422R (clathrin binding mutant)

Myc-epsinR D34G+R67L + D342R+D422R (lipid and clathrin binding mutant)

EpsinR-GFP and ENTH domain overexpression

EpsinR home page

McMahon lab home page