EpsinR D34G + R67L + D342R + D422R overexpression in COS cells

Lipid and clathrin binding mutant
This mutant has a cytoplasmic granular appearance
and points to the role of both lipids and protein interactions in giving the punctate distribution

Blue Channel -
Transferrin
Large Image (41 kb)

Red Channel -
AP1
Large Image (22 kb)

Green Channel -
D34G + R67L + D342R + D422R
Large Image (21 kb)

Merged
Colours as before
Large Image (69 kb)

Colocalisation of EpsinR D34G + R67L + D342R +D422R (green) with markers (red), focusing on the perinuclear region.

M6P receptor
Large Image (79 kb)

Blue - Transferrin
Large Image (65 kb)

EpsinR cover page

Endogenous epsinR expression

Comparison of epsin1 and epsinR

Myc-epsinR (WT) overexpression and colocalisation with markers

Myc-epsinR D34G+R67L (lipid binding mutant)

Myc-epsinR L10E (proposed lipid conformation mutant)

Myc-epsinR D342R+D422R (clathrin binding mutant)

Myc-epsinR D34G+R67L + D342R+D422R (lipid and clathrin binding mutant)

EpsinR-GFP and ENTH domain overexpression

EpsinR home page

McMahon lab home page