EpsinR D34G + R67L + D342R + D422R overexpression in COS cells
Lipid and clathrin binding mutant
This mutant has a cytoplasmic granular appearance
and points to the role of both lipids and protein interactions in giving the punctate distribution
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Red Channel - |
Green Channel - |
Colocalisation of EpsinR D34G + R67L + D342R +D422R (green) with markers (red), focusing on the perinuclear region.
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Clathrin |
M6P receptor |
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GM130 |
TGN46 |
EEA1 |
Blue - Transferrin |
Comparison of epsin1 and epsinR
Myc-epsinR (WT) overexpression and colocalisation with markers
Myc-epsinR D34G+R67L (lipid binding mutant)
Myc-epsinR L10E (proposed lipid conformation mutant)
Myc-epsinR D342R+D422R (clathrin binding mutant)
Myc-epsinR D34G+R67L + D342R+D422R (lipid and clathrin binding mutant)