Comparisons of epsin1 and epsinR overexpression in COS cells

Epsin1 overlaps predominantly with the peripheral clathrin puncta
EpsinR overlaps predominantly with perinuclear clathrin puncta
Both lead to a limited redistribution of clathrin but not like that seen for AP180 C-terminus.
It seems that most of the clathrin staining in the cell can be accounted for by these two epsins

Merge (from epsin1 sup data)
Endogenous EpsinR

Click here for further images of epsin1

EpsinR cover page

Endogenous epsinR expression

Comparison of epsin1 and epsinR

Myc-epsinR (WT) overexpression and colocalisation with markers

Myc-epsinR D34G+R67L (lipid binding mutant)

Myc-epsinR L10E (proposed lipid conformation mutant)

Myc-epsinR D342R+D422R (clathrin binding mutant)

Myc-epsinR D34G+R67L + D342R+D422R (lipid and clathrin binding mutant)

EpsinR-GFP and ENTH domain overexpression

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