Insertion of a glutamic acid (E) in place of the hydrophobic leucine (L) is predicted to affect epsinR's ability to bend membranes. This mutant is not as severe as the lipid binding mutant (D34G +R67L) in that there is co-localisation with AP1 in the perinuclear region but not with GGAs. This mutant is predicted to cause a budding defect.

Click image to view individual channels or proceed to galleries of images below.