This image for day 188 of #LMB365 was collected by Michael Wozny in Wanda Kukulski’s group in Cell Biology using the ion beam source of a Scanning Electron Microscope (SEM). Clumps of plunge-frozen yeast cells are seen centred within the grid squares of an electron microscope grid. The Focussed Ion Beam (FIB) is used to carefully remove material off the tops and bottoms of these cells to produce thin sections called lamellae that are ~200 nm in thickness. These lamellae are then suitable to be imaged using cryo-electron tomography using the LMB’s Titan Krios microscopes.
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