Day 213 of #LMB365 shows a set of 29 DNP-derivatives of amino acids, prepared by Fred Sanger for his work on the sequencing of proteins. This work led to the sequencing of insulin, as reported on this day in 1955, in a landmark paper in the Biochemical Journal, “[this] was essentially the climax of our work on insulin. It was the first protein to be sequenced and we had developed methods and demonstrated their applicability. I think this stimulated interest in sequencing in other laboratories, and our methods were used extensively until better ones were available.” Fred Sanger, Selected papers of Frederick Sanger (with commentaries) World Scientific, 1996. Fred received the first of his two Nobel Prizes in Chemistry for this work.