Day 327 of #LMB365 shows Group Leader Kelly Nguyen preparing some cryo-EM grids. A tiny drop of sample is applied to an EM grid inside a “Vitrobot”. After applying the sample, it is incubated at 4°C and 100% humidity to let the grid adsorb the sample without drying out. After incubation, the “Vitrobot” blots most of the sample away, leaving a thin layer of sample, which is then rapidly plunged into liquid ethane for vitrification.
MRC Laboratory of Molecular Biology
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